EG435970 (BC066874) Mouse Tagged ORF Clone
CAT＃: MG206976
- TrueORF®
EG435970 (tGFP-tagged) - Mouse predicted gene, EG435970 (cDNA clone MGC:76792 IMAGE:30106557)
ORF Plasmid: DDK
"BC066874" in other vectors (4)
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CNY 2760.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EG435970; EG668620; Gm9272; I1C0022H11Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206976 representing BC066874
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCATTGGCCTTTGATGATGTGCATGTACACTTCACTCGGGAGGAGTGGTCCTTGCTGGATCCAT CTCAGAAGAGACTTTACAAAGATGTCATGCTGGAGAACTACAGGAATCTCACCACTATAGGCTACAATTG GAAAGACCATAACATTGAAGAACATTGTCAAAATGATAGAAGATATGGAAGGCACGAAAGAACTCCTGCT GGAGAGAAACCCTATGAATATACACAATGGGATAAAGCCTTCAAATATCACAGTCATCTTCAAAAGCTTG AAAGAAACCAAACTGGAGACAAAAACTCTGAAGTCTTTCAACATAGTAAAGTGCTTGCATGTCAAAGTAC TCTACAAAGAAATAAAAGAACAGATACTAGAGAGAAACCCTACAAATGTAATGAATGTGATAAAGCCTTC TTAAAGCTCAAGTATCTTCGAGTACATGAAAGAACACATTCTGGAAAGAAACCCTATGAATGTAATCAAT GTGGTAAAGCCTTTTTACATCTCAACTATCTTAGAATTCATGAAAGAACACATACTGGAGAGAAACCTTA CCAGTGTAATCAATGTGGTAAAGCCTTTACACAAAGGAGTCATCTTCAAAGCCATAAAGTAGTACATACT GGAGTGAGACCCTACATATGTAATCAGTGTAATAAAGCCTACCCACAGCAGAATTATCTCCGAAAACATG AAAGAACACATACTGGAGAGAAACCTTATATATGTAACCAGTGTAATAAAGCCTTTTCACACCAGAGTAA TCTCCGAATACATGAAAAGACACATACAGGTGAGAAACCCTATAAATGTAATCAGTGTGATAAAGCCTTT TCACACCAGAGTAATCTCCACATACATGAAAGAACACATACAGGTGAGAAACCCTATAAATGTAATCAGT GTGATAAAGCCTTTGCACACCTCAAGTATCTTCGAGCACATGAAAGAACACATACTCAAGAGAAACCATA CAAATGTAATCAATGTGGTAAAGCTTTTTTACATCTCAGCTGTCTTCGGGTACATGAAAGAACACATACT GGAGAGAAACCTTATAAATGTAATCAGTGTGATAAAGCCTTTGCACAGCTCAAGTATCTTCGAGCACATG AAAGAATACATACTCAAGAGAAACCCTACAAATGTAATCAATGTGGTAAAGCCTTTTTACTTCTCAGCTG TCTTCGGGTACATGAAAGAACACATACTGGAGAAAAACCTTACAAATGTGATCAATGTGGTAAGGCTTTT ACACAACGCAGTCATCTTAAAACCCATAAAGTAATACATACTAGAGGGAGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206976 representing BC066874
Red=Cloning site Green=Tags(s) MDALAFDDVHVHFTREEWSLLDPSQKRLYKDVMLENYRNLTTIGYNWKDHNIEEHCQNDRRYGRHERTPA GEKPYEYTQWDKAFKYHSHLQKLERNQTGDKNSEVFQHSKVLACQSTLQRNKRTDTREKPYKCNECDKAF LKLKYLRVHERTHSGKKPYECNQCGKAFLHLNYLRIHERTHTGEKPYQCNQCGKAFTQRSHLQSHKVVHT GVRPYICNQCNKAYPQQNYLRKHERTHTGEKPYICNQCNKAFSHQSNLRIHEKTHTGEKPYKCNQCDKAF SHQSNLHIHERTHTGEKPYKCNQCDKAFAHLKYLRAHERTHTQEKPYKCNQCGKAFLHLSCLRVHERTHT GEKPYKCNQCDKAFAQLKYLRAHERIHTQEKPYKCNQCGKAFLLLSCLRVHERTHTGEKPYKCDQCGKAF TQRSHLKTHKVIHTRGRL TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC066874 |
| ORF Size | 1314 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066874.1 |
| RefSeq Size | 2877 bp |
| RefSeq ORF | 1316 bp |
| Locus ID | 435970 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218717 | EG435970 (untagged) - Mouse predicted gene, EG435970 (cDNA clone MGC:76792 IMAGE:30106557), (10ug) |
CNY 6940.00 |
|
| MR206976 | EG435970 (Myc-DDK-tagged) - Mouse predicted gene, EG435970 (cDNA clone MGC:76792 IMAGE:30106557) |
CNY 3656.00 |
|
| MR206976L3 | Lenti ORF clone of EG435970 (Myc-DDK-tagged) - Mouse predicted gene, EG435970 (cDNA clone MGC:76792 IMAGE:30106557) |
CNY 4370.00 |
|
| MR206976L4 | Lenti ORF clone of EG435970 (mGFP-tagged) - Mouse predicted gene, EG435970 (cDNA clone MGC:76792 IMAGE:30106557) |
CNY 4370.00 |
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