Astl (BC064729) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	7420700F21, Ovastacin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206936 representing BC064729 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTATCATGGGAAGCCTGTGGCCTTGGATATTAACTATGCTCTCCTTGCTAGGTTTGAGCATGGGAG CACCCTCAGCATCCAGATGTTCTGGAGTCTGCAGTACCAGTGTTCCAGAAGGCTTCACTCCTGAGGGAAG CCCGGTATTTCAGGACAAGGACATCCCCGCAATTAACCAAGGGCTCATCTCAGAGGAGACCCCAGAAAGC AGCTTCCTGGTAGAAGGGGACATTATCCGGCCAAGCCCTTTCCGATTGTTGTCAGTGACCAATAATAAAT GGCCCAAGGGCGTTGGTGGCTTTGTGGAGATCCCCTTCCTGCTTTCCAGAAAGTATGATGAACTCAGCCG CCGGGTCATTATGGATGCCTTTGCTGAGTTTGAACGTTTCACATGCATCCGGTTTGTTGCCTACCATGGT CAGAGAGACTTTGTTTCCATTCTTCCTATGGCGGGGTGTTTCTCTGGTGTGGGACGCAGTGGAGGGATGC AGGTGGTGTCCTTGGCACCCACTTGTCTCCGGAAGGGCCGAGGCATTGTCCTACATGAGCTCATGCACGT ACTTGGCTTCTGGCATGAGCATTCACGGGCAGATCGGGACCGCTACATCCAAGTCAACTGGAACGAGATC CTCCCGGGCTTTGAAATCAACTTCATCAAGTCACGGAGTACCAATATGTTAGTTCCCTATGACTACTCAT CTGTGATGCATTATGGGAGATTTGCCTTCAGCTGGCGTGGGCAGCCCACCATCATACCACTCTGGACCTC CAGTGTTCACATTGGCCAGCGATGGAACCTGAGTACCTCAGATATCACCCGGGTCTGCAGGCTGTATAAC TGCAGCCGGAGTGTCCCTGACTCCCACGGGAGAGGGTTTGAGGCCCAGAGTGATGGAAGCAGCCTCACCC CTGCCTCTATATCACGTCTACAAAGACTTCTCGAGGCACTGTCAGAGGAATCTGGAAGCTCTGCCCCTAG TGGCTCCAGGACTGGAGGCCAGAGTATTGCCGGGCTTGGTAACAGCCAGCAAGGATGGGAGCATCCTCCT CAGAGCACATTCAGTGTGGGAGCCTTGGCAAGACCACCTCAGATGCTAGCCGATGCTTCAAAATCGGGGC CTGGAGCAGGTGCAGACAGCTTGTCTCTAGAGCAGTTCCAGCTAGCCCAGGCCCCCACTGTACCTCTTGC TCTATTTCCAGAAGCCAGAGACAAGCCAGCACCTATCCAAGATGCCTTTGAGAGGCTAGCTCCACTTCCA GGAGGCTGTGCACCTGGAAGTCACATTAGAGAGGTGCCCAGAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206936 representing BC064729 Red=Cloning site Green=Tags(s) MGIMGSLWPWILTMLSLLGLSMGAPSASRCSGVCSTSVPEGFTPEGSPVFQDKDIPAINQGLISEETPES SFLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERFTCIRFVAYHG QRDFVSILPMAGCFSGVGRSGGMQVVSLAPTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEI LPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYN CSRSVPDSHGRGFEAQSDGSSLTPASISRLQRLLEALSEESGSSAPSGSRTGGQSIAGLGNSQQGWEHPP QSTFSVGALARPPQMLADASKSGPGAGADSLSLEQFQLAQAPTVPLALFPEARDKPAPIQDAFERLAPLP GGCAPGSHIREVPRD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC064729
ORF Size	1305 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC064729, AAH64729
RefSeq Size	2249 bp
RefSeq ORF	1307 bp
Locus ID	215095
Gene Summary	Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.[UniProtKB/Swiss-Prot Function]

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