Fanci (BC027836) Mouse Tagged ORF Clone
CAT＃: MG206166
- TrueORF®
Fanci (tGFP-tagged) - Mouse Fanconi anemia, complementation group I (cDNA clone IMAGE:5322726)
ORF Plasmid: DDK
"BC027836" in other vectors (4)
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CNY 9120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206166 representing BC027836
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGCACCTTTAATTCTTCAGAACTGTTCAAAAGTCACGGAAACTTTTGACTATTTGACCTTTCTGC CCCTCCAGACCGTGCAAGGGCTGCTCAAGGCGGTGCAGCCGCTTCTCAAGATCAGCATGTCGATGAGAGA CTCCTTGATCCTCGTCTTGCGGAAAGCTATGTTTGCCAGCCAGCTGGATGCCCGGAAATCTGCAGTTGCT GGGTTTTTGCTGCTCCTAAAGAACTTTAAAGTCCTAGGCAGCCTGCCGTCCTCACAGTGCACACAGTCTA TTGGTGTCACGCAGGTGCGCGTGGACGTTCACAGCCGATACAGCGCTGTCGCCAACGAGACCTTCTGCCT TGAGATCATAGACAGCTTGAAACGAAGCTTAGGGCAGCAGGCAGACATCCGGCTCATGCTTTACGACGGT TTCTACGATGTCCTTCGACGGAACTCTCAGCTGGCTAGTTCAATCATGCAGACTCTCTTCTCACAGTTAA AACAGTTCTATGAGCCAGAGCCTGATCTGCTACCTCCTCTGAAACTAGGAGCTTGTGTTCTGACCCAAGG AAGTCAGATCTTTCTACAAGAGCCGCTGGATCATCTGCTGTCTTGCATTCAGCATTGCTTGGCCTGGTAT AAGAGCAGAGTCGTCCCCCTGCAACAAGGAGATGAAGGAGAGGAGGAGGAGGAGGAGTTGTACTCCGAGC TGGATGATATGTTGGAGTCCATTACTGTCAGAATGATCAAGAGTGAGCTGGAAGACTTTGAGCTGGATAA ATCAGCAGATTTCTCTCAGAACACTAATGTCGGCATCAAGAATAATATCTGTGCGTGTCTCATCATGGGA GTGTGTGAAGTTCTAATGGAATATAATTTCTCCATAAGTAACTTCAGCAAGAGTAAGTTTGAGGAGATCC TGAGCCTCTTCACATGTTACAAGAAGTTCTCTGACATCCTCAGTGAGAAAGCTGGCAAAGGCAAGGCTAA GATGACCAGTAAAGTCAGCGACAGTCTCCTGTCCTTGAAGTTTGTGTCTGATCTCCTCACTGCTCTCTTC AGAGACAGCATCCAGAGTCATGAAGAGAGTCTGTCTGTCCTGCGGTCCAGCGGCGAGTTTATGCACTATG CAGTGAACGTCGCTTTGCAGAAGATTCAGCAGCTAATACGAACAGGGCATGTGAGTGGCCCTGATGGTCA GAACCCAGACAAGATCTTTCAGAACCTCTGTGACATAACTCGGGTCCTGCTGTGGAGATACACATCAATT CCTACATCCGTGGAGGAGTCGGGGAAGAAGGAGAAGGGAAAGAGCATCTCGCTGTTGTGCTTGGAGGGCT TGCAGAAGACGTTCAGTGTGGTGCTGCAGTTCTACCAGCCCAAGGTCCAGCAGTTTCTCCAGGCTCTGGA TGTCATGGGCACAGAAGAAGAAGAAGCAGGTGTCACTGTCACTCAGAGGGCCTCGTTCCAGATCCGGCAG TTTCAGAGGTCGTTGTTGAATCTGCTGAGCAGCGAAGAGGACGATTTTAACAGTAAAGAGGCCCTCCTGC TCATCGCGGTGCTGTCCACCTTGTCCAGGCTGCTGGAGCCCACCTCTCCTCAGTTTGTGCAGATGTTATC CTGGACATCTAAGATTTGCAAGGAATACAGCCAGGAGGATGCCTCATTTTGCAAGAGTTTGATGAACCTG TTCTTCAGCCTGCATGTCTTATATAAGAGTCCTGTCACCCTGCTTCGTGACTTGTCCCAGGATATCCATG GGCAGCTGGGAGACATAGATCAAGATGTAGAGATTGAGAAGACAGACCACTTTGCTGTGGTGAATTTGAG AACGGCTGCCCCCACTGTCTGTTTACTTGTTCTGAGTCAGGCTGAGAAAGTCCTAGAAGAAGTGGACTGG TTAATCGCCAAGATCAAGGGCTCAGCAAACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCT CTTCTCAAGCCGTTCCACCAACTCTTCTCATTGAGAAAGCTATCGTCATGCAACTGGGAACTCTGGTAAC GTTCTTCCATGAGCTCGTGCAGACAGCCCTGCCATCAGGCAGCTGTGTAGACACTCTACTGAAGGGCCTG TCCAAGATCTACAGCACCCTAACAGCATTCGTCAAATATTATCTCCAGGTATGTCAGAGCTCCAGAGGAA TTCCAAACACTGTGGAAAAACTGGTGAAGCTGTCGGGCTCTCATCTGACCCCCGTATGTTACTCTTTCAT TTCTTATGTACAAAATAAGAGCAGTGACGCCCCAAAGTGCTCAGAAAAGGAGAAGGCTGCAGTGAGCACA ACCATGGCCAAAGTTCTTCGTGAAACCAAGCCAATCCCTAACCTTGTCTTTGCCATTGAACAGTATGAAA AATTTCTTATCCAACTGTCTAAAAAGTCCAAGGTAAAAATGTTCTTTATTTCCACTCTCATTTACTGCTG GAAGGAGTCTAGCAAGCTCTGTGGCAGGCAGCACAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206166 representing BC027836
Red=Cloning site Green=Tags(s) MYAPLILQNCSKVTETFDYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVA GFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCLEIIDSLKRSLGQQADIRLMLYDG FYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWY KSRVVPLQQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMG VCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDILSEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALF RDSIQSHEESLSVLRSSGEFMHYAVNVALQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRYTSI PTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQ FQRSLLNLLSSEEDDFNSKEALLLIAVLSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNL FFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLVLSQAEKVLEEVDW LIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGL SKIYSTLTAFVKYYLQVCQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVST TMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVKMFFISTLIYCWKESSKLCGRQHS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027836 |
| ORF Size | 2487 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027836 |
| RefSeq Size | 4524 bp |
| RefSeq ORF | 1184 bp |
| Locus ID | 208836 |
| Gene Summary | Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218552 | Fanci (untagged) - Mouse Fanconi anemia, complementation group I (cDNA clone IMAGE:5322726), (10ug) |
CNY 6940.00 |
|
| MR206166 | Fanci (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group I (cDNA clone IMAGE:5322726) |
CNY 8270.00 |
|
| MR206166L3 | Lenti ORF clone of Fanci (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group I (cDNA clone IMAGE:5322726) |
CNY 10170.00 |
|
| MR206166L4 | Lenti ORF clone of Fanci (mGFP-tagged) - Mouse Fanconi anemia, complementation group I (cDNA clone IMAGE:5322726) |
CNY 10170.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
