Rbbp5 (BC057632) Mouse Tagged ORF Clone
CAT＃: MG205771
- TrueORF®
Rbbp5 (tGFP-tagged) - Mouse retinoblastoma binding protein 5 (cDNA clone MGC:67708 IMAGE:4918318)
ORF Plasmid: DDK
"BC057632" in other vectors (4)
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CNY 4370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4933411J24Rik; C330016J05 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205771 representing BC057632
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCTGCTCCTGTCATGTTGACCCTTTCAGATTCCAAACATGTTGTTCTGCCGGTAGACGATGACT CGGATTTGAACGTGGTGGCATCTTTTGATAGGCGAGGGGAATATATTTATACAGGAAATGCAAAAGGCAA GATCTTGGTCCTAAAAACAGACTCTCAGGATCTTGTTGCTTCCTTCAGAGTAACAACTGGGACAAGCAAT ACTACCGCCATTAAGTCAATTGAGTTTGCCCGGAAGGGGAGTTGCTTCCTGATTAACACAGCAGATCGAA TAATAAGAGTCTATGACGGCAGAGAGATTTTAACGTGTGGAAGGGATGGAGAGCCAGAGCCTATGCAGAA GCTGCAGGACTTGGTGAATAGGACTCCATGGAAAAAATGTTGTTTCTCTGGGGATGGGGAGTACATAGTG GCGGGCTCTGCGAGGCAGCATGCGCTGTATATCTGGGAAAAGAGCATTGGCAACCTGGTGAAGATCTTAC ACGGGACCAGAGGGGAACTCCTGCTGGACGTGGCTTGGCATCCAGTCCGACCCATCATAGCTTCTATCTC TAGTGGAGTGGTGTCCATTTGGGCCCAAAATCAAGTAGAAAATTGGAGTGCATTTGCACCAGACTTCAAA GAGTTGGATGAAAATGTAGAATATGAGGAAAGAGAATCAGAATTTGATATTGAGGATGAAGATAAGAGTG AGCCTGAGCAAACAGGGGCTGATGCTGCTGAAGATGAGGAAGTGGATGTCACCAGCGTGGATCCCATCGC TGCCTTCTGTAGCAGTGATGAAGAGCTGGAAGATTCAAAGGCTCTATTGTATTTACCCATTGCCCCTGAG GTAGAAGACCCTGAAGAAAACCCGTATGGCCCTCCACCGGATGCAGTCCCAAGCTCCTTGATGGATGAAG GGGCTAGTTCAGAGAAGAAGAGGCAGTCTTCAGCAGATGGGTCCCAGCCACCAAAGAAGAAACCTAAAAC CACCAATATAGAGCTCCAAGGAGTGCCGAATGATGAAGTCCATCCACTACTGGGTGTGAAGGGGGATGGC AAATCGAAGAAGAAGCAAGCAGGCCGGCCTAAAGGATCAAAAGCAGGAGGAGCCATCTCAGAACTGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205771 representing BC057632
Red=Cloning site Green=Tags(s) MKSAPVMLTLSDSKHVVLPVDDDSDLNVVASFDRRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSN TTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLVNRTPWKKCCFSGDGEYIV AGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSIWAQNQVENWSAFAPDFK ELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKALLYLPIAPE VEDPEENPYGPPPDAVPSSLMDEGASSEKKRQSSADGSQPPKKKPKTTNIELQGVPNDEVHPLLGVKGDG KSKKKQAGRPKGSKAGGAISELL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057632 |
| ORF Size | 1119 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057632, AAH57632 |
| RefSeq Size | 3399 bp |
| RefSeq ORF | 1121 bp |
| Locus ID | 213464 |
| Gene Summary | As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218570 | Rbbp5 (untagged) - Mouse retinoblastoma binding protein 5 (cDNA clone MGC:67708 IMAGE:4918318), (10ug) |
CNY 5488.00 |
|
| MR205771 | Rbbp5 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 5 (cDNA clone MGC:67708 IMAGE:4918318) |
CNY 5488.00 |
|
| MR205771L3 | Lenti ORF clone of Rbbp5 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 5 (cDNA clone MGC:67708 IMAGE:4918318) |
CNY 5890.00 |
|
| MR205771L4 | Lenti ORF clone of Rbbp5 (mGFP-tagged) - Mouse retinoblastoma binding protein 5 (cDNA clone MGC:67708 IMAGE:4918318) |
CNY 5890.00 |
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