Mbnl2 (BC075665) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MBLL, mKIAA4072
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205765 representing BC075665 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGAACGTTGCCCCCGTGAGAGACACAAAGTGGCTGACGCTGGAGGTCTGCAGACAGTACCAGA GAGGAACGTGCTCACGCTCCGACGAAGAATGCAAGTTTGCTCACCCCCCCAAAAGTTGCCAGGTTGAAAA TGGAAGAGTAATTGCCTGCTTTGATTCCCTCAAGGGCCGCTGTTCAAGAGAGAACTGCAAATATCTTCAT CCTCCGACACACTTAAAAACCCAGCTAGAGATTAATGGGAGGAACAATTTGATCCAGCAAAAAACTGCAG CAGCGATGCTTGCCCAGCAGATGCAATTTATGTTTCCAGGAACGCCGCTCCATCCTGTGCCCACTTTTCC TGTAGGTCCCACCATAGGGACAAATGCGGCTATTAGCTTTGCTCCTTACTTAGCGCCTGTCACCCCTGGA GTTGGGTTAGTCCCAACAGAGGTTCTACCCACTACACCGGTCATTGTTCCCGGAAGTCCACCGGTCACTG TCCCGGGCTCAACTGCAACTCAGAAACTTCTCAGGACTGATAAACTGGAGGTATGCAGGGAGTTCCAGCG AGGAAACTGTGCCCGGGGAGAGACAGACTGCCGCTTTGCACACCCGGCAGACAGCACCATGATCGACACA AACGACAACACCGTAACCGTTTGTATGGATTACATAAAGGGGCGTTGCATGAGGGAGAAATGCAAATATT TTCACCCTCCTGCACACTTGCAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAGGCCGCGGTGGC CGCCCAGGCAGCCGCGGCCGCGGCCACAGTCATGACTCAGTCGACTGCCAAAGCACTGAAGCGACCTCTC GAAGCAACTGTAGACCTGGCCTTCCCTCCGGGTGCTCTTCATCCCTTACCAAAGAGACAAGCACTTGAAA AAAGCAACGGGGCCAGCACGGTCTTCAACCCCAGCGTCTTGCACTACCAGCAGGCTCTGACCAGTGCGCA GCTGCAGCAGCACACGGCGTTCATCCCCACAGTACCCATGATGCACAGCGCTACGTCCGCCACTGTCTCT GCAGCAACAACTCCTGCAACAAGTGTCCCCTTCGCAGCAACAGCCACAGCCAATCAGATAATTCTGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205765 representing BC075665 Red=Cloning site Green=Tags(s) MALNVAPVRDTKWLTLEVCRQYQRGTCSRSDEECKFAHPPKSCQVENGRVIACFDSLKGRCSRENCKYLH PPTHLKTQLEINGRNNLIQQKTAAAMLAQQMQFMFPGTPLHPVPTFPVGPTIGTNAAISFAPYLAPVTPG VGLVPTEVLPTTPVIVPGSPPVTVPGSTATQKLLRTDKLEVCREFQRGNCARGETDCRFAHPADSTMIDT NDNTVTVCMDYIKGRCMREKCKYFHPPAHLQAKIKAAQHQANQAAVAAQAAAAAATVMTQSTAKALKRPL EATVDLAFPPGALHPLPKRQALEKSNGASTVFNPSVLHYQQALTSAQLQQHTAFIPTVPMMHSATSATVS AATTPATSVPFAATATANQIILK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC075665
ORF Size	1119 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC075665, AAH75665
RefSeq Size	2099 bp
RefSeq ORF	1121 bp
Locus ID	105559
Gene Summary	Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. RNA-binding protein that binds to 5'ACACCC-3' core sequence, termed zipcode, within the 3' UTR of ITGA3. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs. Seems to regulate expression and localization of ITGA3 by transporting it from the nucleus to cytoplasm at adhesion plaques. May play a role in myotonic dystrophy pathophysiology (DM) (By similarity).[UniProtKB/Swiss-Prot Function]

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