Sgpp2 (BC049995) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SPPase2, Spp2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205386 representing BC049995 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAACTGCTGCGGAGCCTGCGGGATTCACAGCTCGTGGCGCGCTTCCAGCGCCGCTGCGGGCTCT TCCCAGCGCGGGAGGCTTCTGGAGAGGAGCATGTGGTGAAGAATTATTTCTACTATTACCTGTTCCGGTT TTCAGCCGCTTTGGGCCAGGAAGTGTTCTACATCACGTTTCTCCCTTTCACCCACTGGAATATCGACCCT AATTTGTCCAGAAGATTGGTTGTGATATGGGTTTTGGTGATGTATATTGGCCAGGTGGCCAAGGACATCC TGAAGTGGCCCCGGCCCTCCTTTCCTCCCGTTGTGAGACTTGAAAAGAGAATTATTGCAGAGTACGGAAT GCCATCCACCCACGCCATGGCGGCCACCGCCATCTCCTTCACCCTCCTCATCTCTACCATGGACCGGTAT CAGTACCCTTTTATTTTAGGCCTGATGATGGCTGTGGTGTTCTCTACGCTGGTGTGTCTCAGCAGACTCT ACACGGGGATGCACACGGTCCTGGATATCCTGGGTGGCGTTCTGATCACTGCAGTCCTCATTGCTCTGAC CTACCCTGCCTGGACCCTCATCGACTCCCTGGACTCTGCCAGCCCCCTCTTCCCTGTGTGTGTCATAGTT GTACCGTTCCTCTTGTGCTACAATTACCCTGTGTCTGACTACTACAGCCCGACCAGGGCAGACACCACCA CCATCGTGGCTGCTGGGGCTGGAGTGACCCTGGGATTCTGGATCAACCATTTCTTCCAGCTCGTGTCCAA GCCCACCCCATCTCTTCCTGTCATTCAGAACATCCCACCCCTTACCACTGACATGCTGGTTTTAGGTCTG ACCAAATTTATGGTGGGAATCATGTTGATCCTCTTGGTTCGCCAACTGGTGCAAAAGCTCTCCCTGCAAG TATTATTCTCATGGTTCAAGGTGGTTACTAGGAACAAGGAGGCCAGGCGAAGACTAGAGATTGAAGTCCC TTACAAGTTTGTTACCTACACATCTGTTGGCATCTGTGCCACAACCTTTGTGCCAATGTTGCACAGGTTT TTGGGATTGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205386 representing BC049995 Red=Cloning site Green=Tags(s) MAELLRSLRDSQLVARFQRRCGLFPAREASGEEHVVKNYFYYYLFRFSAALGQEVFYITFLPFTHWNIDP NLSRRLVVIWVLVMYIGQVAKDILKWPRPSFPPVVRLEKRIIAEYGMPSTHAMAATAISFTLLISTMDRY QYPFILGLMMAVVFSTLVCLSRLYTGMHTVLDILGGVLITAVLIALTYPAWTLIDSLDSASPLFPVCVIV VPFLLCYNYPVSDYYSPTRADTTTIVAAGAGVTLGFWINHFFQLVSKPTPSLPVIQNIPPLTTDMLVLGL TKFMVGIMLILLVRQLVQKLSLQVLFSWFKVVTRNKEARRRLEIEVPYKFVTYTSVGICATTFVPMLHRF LGLL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC049995
ORF Size	1062 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049995, AAH49995
RefSeq Size	4030 bp
RefSeq ORF	1064 bp
Locus ID	433323
Gene Summary	Has specific phosphohydrolase activity towards sphingoid base 1-phosphates. Has high phosphohydrolase activity against dihydrosphingosine-1-phosphate and sphingosine-1-phosphate (S1P) in vitro (Probable). Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway (By similarity). May play a role in attenuating intracellular sphingosine 1-phosphate (S1P) signaling. May play a role in pro-inflammatory signaling (By similarity). Plays a role in the regulation of pancreatic islet beta-cell endoplasmic reticulum stress and proliferation (PubMed:27059959).[UniProtKB/Swiss-Prot Function]

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