Nde1 (BC023267) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810027M15Rik; AU042936; AW822251; mNudE; Nude
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205167 representing BC023267 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTCGGGAAAGACCTTTGAATCAGAGGAGGAAGAAACAAACTATTGGAGAGACCTGGCCATGA CCTACAAACAGAGGGCAGAGAATACTCAGGAAGAACTCAGAGAGTTCCAGGAAGGAAGCCGAGAATACGA AGCTGAATTGGAGGCTCAGCTGCAGCAGATTGAAACCAGGAACCGGGACCTCTTGTCAGAGAATAACCGC CTTCGCATGGAGCTGGAGTCTGTGAAGGAGAAGTTTGAGATGCAGCACTCAGAGGGTTACCGGCAGATCT CAGCCTTGGAGGATGACCTGGCGCAGACGAAAGCCATTAAAGACCAACTGCAGAAATACATTAGGGAACT GGAACAAGCCAATGATGACCTGGAAAGAGCCAAACGAGCCACAATCATGTCCCTGGAAGACTTTGAGCAG CGTTTGAATCAAGCCATTGAAAGAAATGCCTTCCTAGAGAGTGAGCTGGATGAGAAGGAGAATCTTCTAG AATCTGTGCAGAGGCTGAAGGATGAAGCCCGAGATCTGCGGCAGGAATTGGCTGTGCAACAGAAGCAAGA CAAGCCCCGGACACCCATGCCAGGCTCAGGGCAAGCCAAGAGGACAGACATGGCTGTGCAGGCCACAGGC TCTGTACCGTCTACTCCAGTAGCTCACCGAGGACCTAGCTCTGGTTTGAACACACCAGGAATGTTCAGAC GTGGTCTGGACAGCTCCACTAGTGGGACGCCACTCACACCTGCAGCCCGGATATCAGCCCTAAACATCGT TGGGGACCTGCTTCGGAAAGTTGGGGCCCTGGAGTCCAAGCTAGCATCATGCAGGAACTTCATGTATGAT CAGTCCCCAAGCCGGACAAGTGGGCCAGCCTCAGGACGAGGAACCAAAAACAGAGATGGTGTTGACAGAA GGCCAGGCAGTACCAGTGTGGGCGATAAAGGGTCAGGAAAACGCTTGGAGTTTGGGAAGCCAGCCTCAGA GCCAGCATCACCAGCGCTACCATCCGCCCAGGGTGTGGTCAAGCTTTTGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205167 representing BC023267 Red=Cloning site Green=Tags(s) MEDSGKTFESEEEETNYWRDLAMTYKQRAENTQEELREFQEGSREYEAELEAQLQQIETRNRDLLSENNR LRMELESVKEKFEMQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQ RLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQDKPRTPMPGSGQAKRTDMAVQATG SVPSTPVAHRGPSSGLNTPGMFRRGLDSSTSGTPLTPAARISALNIVGDLLRKVGALESKLASCRNFMYD QSPSRTSGPASGRGTKNRDGVDRRPGSTSVGDKGSGKRLEFGKPASEPASPALPSAQGVVKLLL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC023267
ORF Size	1032 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC023267, AAH23267
RefSeq Size	2196 bp
RefSeq ORF	1034 bp
Locus ID	67203
Gene Summary	Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.[UniProtKB/Swiss-Prot Function]

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