Hsd17b6 (BC021836) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Rdh8, 17betaHSD9
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204561 representing BC021836 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTTCTACCTGGTAACTCTTGTGGGCCTTTACCACCTTCTGCGTTGGTATCGTGAGAGGCAGGTGG TGAGCCATCTCCAAGACAAGTATGTCTTCATCACGGGCTGTGACTCTGGCTTTGGGAACCTGCTGGCCAG ACAGCTGGACAGGAGAGGCATGAGGGTATTGGCTGCATGTCTGACGGAGAAGGGAGCCGAGGAGCTGAGG AACAAGACATCTGACAGGCTGGAGACAGTGATCCTGGATGTCACCAAGACAGAGAGTATTGTGGCAGCCA CTCAGTGGGTGAAGGAGCGTGTTGGAGACAGAGGACTCTGGGGTTTGGTTAATAATGCAGGTGTGTTACA ACCATTTGCCTACATTGAATGGTACAGACCAGAGGACTACATGCCTATCTTTCAAGTGAACCTCATTGGT TTGACCCAGGTGACTATAAGCATGCTTTTCCTGGTAAAGAAGGCTCGGGGCAGGATCGTCAATGTCTCCA GTGCTTTGGGAAGAGTTGCATTGTTTGGAGGATTCTACAGTTGCTCCAAGTATGGGGTTGAGGCATTTTC AGATGTGCTAAGGCATGAGGTTCAAGATTTCGGGGTGAAAGTCAGCATAATTGAACCTGGGAGCTTCAAG ACGGAAATGACAGATGCAGAGTTAACCATTGAGAGAACTAAGAAAGTCTGGGAAGCTGCCCCTGAGCACA TCAAGGAATCCTACGGACAGCAGTTTTTTGACGACTTTTGCAGCACCACCAAACGAGAGTTGATGAAGTG TAGCAGGAACCTGAGCCTAGTCACGGACTGCATGGAGCACGCCCTGACCTCCACGCATCCTCGCACCCGG TACTCGGCTGGCTGGGATGCCAAGTTTTTCTTCATCCCTCTATCTTATTTGCCTGCGTCACTGGTAGACT ACTTATTGGCCATATCTAGGGGCAAGCCAGCTCAAGCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204561 representing BC021836 Red=Cloning site Green=Tags(s) MWFYLVTLVGLYHLLRWYRERQVVSHLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTEKGAEELR NKTSDRLETVILDVTKTESIVAATQWVKERVGDRGLWGLVNNAGVLQPFAYIEWYRPEDYMPIFQVNLIG LTQVTISMLFLVKKARGRIVNVSSALGRVALFGGFYSCSKYGVEAFSDVLRHEVQDFGVKVSIIEPGSFK TEMTDAELTIERTKKVWEAAPEHIKESYGQQFFDDFCSTTKRELMKCSRNLSLVTDCMEHALTSTHPRTR YSAGWDAKFFFIPLSYLPASLVDYLLAISRGKPAQAA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC021836
ORF Size	951 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021836, AAH21836
RefSeq Size	1479 bp
RefSeq ORF	953 bp
Locus ID	27400
Gene Summary	NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro).[UniProtKB/Swiss-Prot Function]

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