AI118078 (BC068128) Mouse Tagged ORF Clone
CAT＃: MG204132
- TrueORF®
AI118078 (tGFP-tagged) - Mouse expressed sequence AI118078 (cDNA clone MGC:92972 IMAGE:6835202)
ORF Plasmid: DDK
"BC068128" in other vectors (4)
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CNY 2850.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9630029F15; AI118078; HVRP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204132 representing BC068128
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTATTTCAGCCACACCGTGCTTTCAGGTCTGACTGGCTGAAGCCGTGCTGTGGGAAGAGAGCAG CCGTATGGCAGGTATTTTTGCTCAGTGCAAGTCTCAACAGTTTCCTGGTAGCCTGTGTAATATTGGTGGT GATCCTCCTGACTCTGGAGCTTCTCATAGATACAAAGCTTCTCCAGTTTTCCAATGCTTTCCAGTTTGCT GGTGTCATTCACTGGATCAGTCTGGTCATTCTCTCTGTGTTCTTCTCAGAGACTGTCCTACGGATCGTGG TACTGGGGATCTGGGATTACATCGAAAACAAAATAGAGGTGTTTGACGGGGCTGTGATCATCCTGTCCTT GGCCCCGATGGTGGCGTCCACTGTGGCTAACGGACCCAGGAGCCCCTGGGATGCCATCAGCCTCATCATC ATGTTCCGAATCTGGCGGGTGAAGAGGGTCATTGATGCCTATGTCCTGCCAGTCAAGTTGGAGATGGAGA TGGTCACCCAGCAGTATGAGAAGGCCAAGGCCATCCAAGATGAGCAGCTGGAAAGACTGACGCAAATCTG TCAGGAGCAAGGGTTTGAGATCCGGCAGCTGCGTGCGCACCTGGCACAGCAGGACCTGGATCTGGCAGCC GAGCGGGAGGCGGCGCTGCAGGCCCCACACGTGCTCAGCCAGCCACGCAGCCGCTACAAGGTCGTAGAGG CTGGCACGTGGGCCGAGGAGACAGCAGCCGAGAGCATCGTGGAAGAGCTGAGGCCCTCTCAAGAAGCCAC AGTGAAAGATGACATGAACAGCTACATCAGCCAATACTACAATGGGCCCAGCAGTGGTAAGTCTGGGTTG GGACTGTTTGCATGCACTATGCAGCCCCAGGACACTTGTGACCCCAGCAAACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204132 representing BC068128
Red=Cloning site Green=Tags(s) MMLFQPHRAFRSDWLKPCCGKRAAVWQVFLLSASLNSFLVACVILVVILLTLELLIDTKLLQFSNAFQFA GVIHWISLVILSVFFSETVLRIVVLGIWDYIENKIEVFDGAVIILSLAPMVASTVANGPRSPWDAISLII MFRIWRVKRVIDAYVLPVKLEMEMVTQQYEKAKAIQDEQLERLTQICQEQGFEIRQLRAHLAQQDLDLAA EREAALQAPHVLSQPRSRYKVVEAGTWAEETAAESIVEELRPSQEATVKDDMNSYISQYYNGPSSGKSGL GLFACTMQPQDTCDPSKP TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC068128 |
| ORF Size | 894 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068128, AAH68128 |
| RefSeq Size | 4432 bp |
| RefSeq ORF | 896 bp |
| Locus ID | 244886 |
| Gene Summary | Voltage-sensor protein present on the post-synaptic side of glutamatergic mossy fibers and granule cells in the cerebellum. Despite the presence of a voltage-sensor segment, does not form a functional ion channel and its precise role remains unclear. Undergoes both rapid and slow structural rearrangements in response to changes in voltage. Contains a zinc-binding site that can regulate the slow conformational transition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218039 | AI118078 (untagged) - Mouse expressed sequence AI118078 (cDNA clone MGC:92972 IMAGE:6835202), (10ug) |
CNY 6940.00 |
|
| MR204132 | AI118078 (Myc-DDK-tagged) - Mouse expressed sequence AI118078 (cDNA clone MGC:92972 IMAGE:6835202) |
CNY 2400.00 |
|
| MR204132L3 | Lenti ORF clone of AI118078 (Myc-DDK-tagged) - Mouse expressed sequence AI118078 (cDNA clone MGC:92972 IMAGE:6835202) |
CNY 4750.00 |
|
| MR204132L4 | Lenti ORF clone of AI118078 (mGFP-tagged) - Mouse expressed sequence AI118078 (cDNA clone MGC:92972 IMAGE:6835202) |
CNY 4750.00 |
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