Fbxo17 (BC026470) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Fbg4; Fbx17; Fbxo26
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203902 representing BC026470 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCGCGGCCCTCGCGACGGCAGATGACTGAAGCTCGGCGCCTGGCTCTAACCAAGCTGCCCCCTG AGCTGCTGGTGCAGGTGCTGAGCCACGTGCCGCCTCGCGCGTTAGTGACCCGCTGCCGGCCAGTGTGCCG GGCCTGGCGCGACCTGGTAGATGGCCCAAGTATATGGCTGCTGCAACTGGCTCGCGACCGCAGCGCGGAG GGCAGAGCCCTCTACGCCTTGGCCCAGCGCTGTCCCGCCGACGCCGACGCCGACGCCGACGCCGACGGCA ACCGCCACGACGAGTTCCCATTCTGCGCTCTGGCCCGATTCTGCCTGAGAGCGCCGTTTGGCCGCAACCT CATCCACAACTCCTGCGGAGAACAGGGCTTCAGAGGTTGGGAAGTGGAGCACGGCGGAAATGGTTGGGCC GTGGAAAAGAATTTGACGCTGGTACCAGGGGCTCCTTCCCAGACCTGCTTTGTGACTTCTTTTGAGTGGT GCTCCAAGAGGCAGCTTGTAGACCTGGTGAAGGAAGGAGTATGGCAGGAGCTGTTGGACAGTGGCCAGAT TGAGATCTGCATAGCTGACTGGTGGGGCGCCCGTGAGAACTGCGGCTGCATCTACCGGCTTCGAGTTCGC CTACTGGATGAATATGAAAACGAAGTGGTCAAGTTCTCTGCCTCACCCAACCCAGTCCTTCAGTGGACTG AGAGGAGCTGCCGACAAGTCTCTCACGTCTTCACTAACTTTGGCAAGGGCATCCGGTATGTGTCTTTTGA GCAGTATGGGAGAGACACGCGTTCCTGGGTGGGACACTACGGTGCCCTTGTAACTCACTCCAGTGTGCGG GTCAGGATCCGTCTGTCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203902 representing BC026470 Red=Cloning site Green=Tags(s) MGARPSRRQMTEARRLALTKLPPELLVQVLSHVPPRALVTRCRPVCRAWRDLVDGPSIWLLQLARDRSAE GRALYALAQRCPADADADADADGNRHDEFPFCALARFCLRAPFGRNLIHNSCGEQGFRGWEVEHGGNGWA VEKNLTLVPGAPSQTCFVTSFEWCSKRQLVDLVKEGVWQELLDSGQIEICIADWWGARENCGCIYRLRVR LLDEYENEVVKFSASPNPVLQWTERSCRQVSHVFTNFGKGIRYVSFEQYGRDTRSWVGHYGALVTHSSVR VRIRLS SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026470
ORF Size	858 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026470, AAH26470
RefSeq Size	1977 bp
RefSeq ORF	860 bp
Locus ID	50760
Gene Summary	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. Also recognizes sulfated glycans. Does not bind high-mannose glycoproteins (By similarity).[UniProtKB/Swiss-Prot Function]

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