Mapre3 (NM_133350) Mouse Tagged ORF Clone
CAT#: MG203812
- TrueORF®
Mapre3 (tGFP-tagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3)
ORF Plasmid: DDK
"NM_133350" in other vectors (4)
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CNY 2,850.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI790651; EB2; EB3; EBF3; RP3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203812 representing NM_133350
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTCAATGTGTACTCCACTTCTGTGACCAGTGAGAATCTGAGTCGCCATGATATGCTTGCATGGG TCAATGACTCTCTGCACCTCAATTATACCAAGATTGAACAGCTCTGTTCAGGGGCAGCCTACTGCCAGTT CATGGACATGCTCTTCCCTGGCTGTGTTCACTTGAGGAAGGTCAAGTTCCAGGCCAAACTAGAACACGAG TACATCCACAACTTCAAGGTGCTGCAAGCAGCTTTCAAGAAGATGGGTGTTGACAAAATCATTCCCGTAG AGAAGTTAGTGAAAGGAAAATTCCAAGATAATTTTGAGTTTATACAGTGGTTTAAGAAATTCTTTGACGC AAACTATGATGGAAAGGATTACAACCCTCTGCTGGCGCGGCAGGGCCAGGACGTAGCACCACCTCCTAAC CCAGGTGATCAGATCTTCAACAAATCCAAGAAACTCATTGGCACAGCAGTTCCGCAGAGGACGTCCCCCA CAGGCCCCAAGAACATGCAGACCTCTGGACGACTCAGCAACGTGGCTCCGCCCTGCATCCTCCGGAAGAA TCCCCCCTCAGCCCGAAACGGTGGCCATGAGGCTGACGCCCAGATCCTCGAGCTTAACCAGCAGCTGCTG GACTTGAAGCTGACCGTAGACGGGCTTGAGAAAGAACGAGATTTCTATTTCAGCAAATTGCGAGACATCG AGCTGATCTGCCAGGAACATGAGAGCGAGAACAGCCCCGTCATCTCGGGCATCATTGGCATTCTCTATGC CACGGAGGAGGGATTTGCACCCCCTGAGGATGATGAGATTGAAGAACACCAACAGGAAGACCAGGACGAG TAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203812 representing NM_133350
Red=Cloning site Green=Tags(s) MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHE YIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPN PGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHEADAQILELNQQLL DLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDE Y TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133350 |
ORF Size | 843 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133350.2, NP_579928.1 |
RefSeq Size | 1921 bp |
RefSeq ORF | 846 bp |
Locus ID | 100732 |
UniProt ID | Q6PER3 |
Gene Summary | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (By similarity). May play a role in cell migration (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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HDAC6 and RhoA are novel players in Abeta-driven disruption of neuronal polarity
,Tsushima, H;Emanuele, M;Polenghi, A;Esposito, A;Vassalli, M;Barberis, A;Difato, F;Chieregatti, E;,
Nat Commun
,PubMed ID 26198811
[MAPRE3]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212060 | Mapre3 (untagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3), (10ug) |
CNY 3,990.00 |
|
MR203812 | Mapre3 (Myc-DDK-tagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3) |
CNY 1,416.00 |
|
MR203812L3 | Lenti ORF clone of Mapre3 (Myc-DDK-tagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3) |
CNY 4,750.00 |
|
MR203812L4 | Lenti ORF clone of Mapre3 (mGFP-tagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3) |
CNY 4,750.00 |