Crybb1 (BC029012) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	3110006K12Rik; BB1CRY
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203610 representing BC029012 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGGCTGCCAAGGCTTCAGCCACCACAGCGGTGAACCCAGGGCCTGATGGCAAGGGAAAAGGGG CCCCATCCACAGGGCCAGCCCCTGCCCCTGGCCCCACTCCTGTCCCAGCTTCTGTGCCCAGGCCCGCTGC CAAAGTAGGGGACCTGCCTCCTGGGAGCTACAGGCTGATCGTCTTCGAGCAGGAAAACTTTCAGGGCCGC CGGGTGGAATTCTCGGGGGAGTGCCTGAATCTGGGGGACCGTGGCTTCGACAGAGTGCGCAGCCTGATCG TCGTCTCGGGACCCTGGGTGGCCTTTGAGCAATCTGCCTTCCGTGGAGAGATGTTTGTCCTGGAGAAGGG CGAGTACCCACGCTGGGACACCTGGACCAGCAGTTACCGCAGTGACCGGCTCATGTCCTTCCGGCCCATC AGGATGGATTCCCAGGAACATAAGATCTGCCTGTTCGAAGGGGCCAACTTCAAGGGCAACACCATGGAGA TCCAAGAGGATGATGTGCCCAGCCTCTGGGTTTATGGCTTCTGTGACCGTGTGGGCAGCATAACCGTCTC TGGTGGCACATGGGTCGGCTACCAGTACCCAGGTTACCGTGGCTACCAGTATCTGCTGGAGCCTGGCGAC TTCCGGCACTGGAACGAGTGGGGCGCCTTCCAGCCGCAGATGCAGGCTGTGCGGCGCCTGCGGGACCGCC AGTGGCACCAAGAGGGCTGCTTCCCGGTGCTGACAGCCGAGCCCCCCCAAGTGAGACAAGCTCCCGGCCT CCCTTCTACAGCCCGCCCTGCCTCACGTCCCATCTTCCCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203610 representing BC029012 Red=Cloning site Green=Tags(s) MSQAAKASATTAVNPGPDGKGKGAPSTGPAPAPGPTPVPASVPRPAAKVGDLPPGSYRLIVFEQENFQGR RVEFSGECLNLGDRGFDRVRSLIVVSGPWVAFEQSAFRGEMFVLEKGEYPRWDTWTSSYRSDRLMSFRPI RMDSQEHKICLFEGANFKGNTMEIQEDDVPSLWVYGFCDRVGSITVSGGTWVGYQYPGYRGYQYLLEPGD FRHWNEWGAFQPQMQAVRRLRDRQWHQEGCFPVLTAEPPQVRQAPGLPSTARPASRPIFPY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC029012
ORF Size	813 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029012, AAH29012
RefSeq Size	894 bp
RefSeq ORF	815 bp
Locus ID	12960
Gene Summary	This gene encodes a member of the crystallin family of proteins that contribute to the transparency and refractive properties of the ocular lens. The encoded protein associates with other beta crystallin proteins to form dimers, tetramers and other higher-order complexes. This gene is located adjacent to a related crystallin gene on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

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