Nt5c3l (BC015307) Mouse Tagged ORF Clone
CAT＃: MG203318
- TrueORF®
Nt5c3l (tGFP-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117)
ORF Plasmid: DDK
"BC015307" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610037D24Rik; AI841843; C330027I04Rik; Nt5c3l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203318 representing BC015307
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGAGCAGATTTGCTTACAATGGACAGCGATGCCCTTCTTCCCACAATATTCTGGATAACAGCA AAATTATCAGTGAGGATTGTCGCAAAGAGCTCACCGAGCTCTTTCACCACTATTATCCCATTGAGATCGA CCCACACCGGACCATCAAAGAGAAGCTGCCTCACATGGTGCAGTGGTGGAGCAAAGCCCACAGTCTCCTG TGCCAGCAGAGGATCCAGAAAGTCCAGATCGCTCAGGTGGTTGGAGAGTCCACTGCGATGCTCAGGGAGG GCTATAAGACGTTCTTCGACACACTCTACCAGAACAACATCCCCCTTTTCATCTTCTCAGCGGGCATTGG TGACATCCTGGAAGAAATTATCCGACAGATGAAAGTGTTCCACCCCAACATCCACATTGTGTCCAACTAC ATGGACTTCAGTGAGGATGGATTCCTGAAAGGATTCAAGGGGCAGCTCATTCACACCTACAACAAGAACA GCTCCGTGTGTGAGAACTCCAGCTACTTCCAGCAACTTCAGAACAAGACCAACATCATCCTGCTGGGAGA CTCCATCGGGGACCTCACCATGGCTGATGGGGTCCCCGGGGTGCAGAACATTCTCAAGATCGGCTTCCTG AATGACAAGGTGGAGGAGCGGCGGGAGCGTTACATGGACTCCTACGACATTGTGTTGGAGAAGGACGAGA CGCTGGACGTGGTTAATGGGTTGCTGCGGCACATCCTTTACCAGGGGGACTGCGTGGAGCTCCAGGGCTC T ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203318 representing BC015307
Red=Cloning site Green=Tags(s) MTLSRFAYNGQRCPSSHNILDNSKIISEDCRKELTELFHHYYPIEIDPHRTIKEKLPHMVQWWSKAHSLL CQQRIQKVQIAQVVGESTAMLREGYKTFFDTLYQNNIPLFIFSAGIGDILEEIIRQMKVFHPNIHIVSNY MDFSEDGFLKGFKGQLIHTYNKNSSVCENSSYFQQLQNKTNIILLGDSIGDLTMADGVPGVQNILKIGFL NDKVEERRERYMDSYDIVLEKDETLDVVNGLLRHILYQGDCVELQGS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC015307 |
| ORF Size | 771 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC015307, AAH15307 |
| RefSeq Size | 1390 bp |
| RefSeq ORF | 773 bp |
| Locus ID | 68106 |
| Gene Summary | Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217850 | Nt5c3l (untagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117), (10ug) |
CNY 6940.00 |
|
| MR203318 | Nt5c3l (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117) |
CNY 2400.00 |
|
| MR203318L3 | Lenti ORF clone of Nt5c3l (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117) |
CNY 4750.00 |
|
| MR203318L4 | Lenti ORF clone of Nt5c3l (mGFP-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117) |
CNY 4750.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
