Thoc7 (BC054419) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Nif3l1bp1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202136 representing BC054419 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCGTGACTGACGACGAAGTTATACGGAAACGTCTTCTGATTGATGGAGATGGTGCTGGGGATG ATCGGAGAATTAATCTCCTCGTGAAAAGCTTCATTAAATGGTGCAACTCTGGATCCCAAGAGGAAGGGTA TAGCCAGTACCAACGCATGCTGAGCACTCTGTCCCAGTGTGAATTTTCAATGGGCAAAACATTGCTGGTA TATGATATGAATCTCAGAGAAATGGAAAATTATGAAAAAATATACAAAGAAATAGAATGTAGTATTGCTG GAGCACATGAAAAAATTGCTGAGTGTAAAAAGCAGATTCTTCAAGCAAAACGAATACGAAAAAATCGACA AGAATATGACGCTTTGGCCAAAATGATCCAGCATCACCCAGATAGGCATGAGACACTGAAGGAGCTAGAG GCTCTGGGCAAAGAATTAGAGCATCTCTCACATATTAAAGAAAGTGTTGAAGATAAGCTGGAATTGAGAC GGAAACAATTTCACGTTCTTCTTAGTACCATCCATGAACTTCAACAGACATTGGAGAATGATGACAAGCT GTCAGAGGTGGATGAAGCTCAAGAAAGCACCATGGAAGCAGACCCTAAGCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202136 representing BC054419 Red=Cloning site Green=Tags(s) MGAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFIKWCNSGSQEEGYSQYQRMLSTLSQCEFSMGKTLLV YDMNLREMENYEKIYKEIECSIAGAHEKIAECKKQILQAKRIRKNRQEYDALAKMIQHHPDRHETLKELE ALGKELEHLSHIKESVEDKLELRRKQFHVLLSTIHELQQTLENDDKLSEVDEAQESTMEADPKP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC054419
ORF Size	612 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054419, AAH54419
RefSeq Size	993 bp
RefSeq ORF	614 bp
Locus ID	66231
Gene Summary	Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway.[UniProtKB/Swiss-Prot Function]

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