9430016H08Rik (BC024945) Mouse Tagged ORF Clone
CAT＃: MG201651
- TrueORF®
9430016H08Rik (tGFP-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472)
ORF Plasmid: DDK
"BC024945" in other vectors (4)
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CNY 2850.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA409999; C78988 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201651 representing BC024945
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCATTCTGGGATTTTCCAATCCCATCAACTGGGTCCGGACTCGAATTTACGCCTTCCTTATTTGGG CCTATTTCGACAAAGAGTTCAGTATCGCTGAGTTCTCTGAAGGGGCGAAGCAGGCTTTTGCTTATGTATC CAAGTTGCTGTCACAGTGTAAATTTGATCTACTGGAAGAACTTGTAGCCAAAGAGGTGCTACAGATATTA AAAGAAAAAGTTACTTCATTGTCTGATAACCATAAGAATGCACTTGCTGCAGACATAGATGACATTGTCT ACACATCAACAGGCGATATCTCCATTTACTATGATGAGAAAGGAAGGAAGTTTGTTAACATCCTGATGTG CTTTTGGTATCTGACTAGTGCCAACATCCCCAGTGAGTCTTTAAGTGGAGCCAATGTGTTCCAGGTGAAG CTGGGGGATCAGAGTGTGGAAACTAAACAGCTGCTCAGTGCAAGCTATGAATTTCAGAGGGAATTCACAC AAGGAGTAAAGCCTGATTGGACGATTGCGCGGATTGAACACTCCAAGTTACTAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201651 representing BC024945
Red=Cloning site Green=Tags(s) MIILGFSNPINWVRTRIYAFLIWAYFDKEFSIAEFSEGAKQAFAYVSKLLSQCKFDLLEELVAKEVLQIL KEKVTSLSDNHKNALAADIDDIVYTSTGDISIYYDEKGRKFVNILMCFWYLTSANIPSESLSGANVFQVK LGDQSVETKQLLSASYEFQREFTQGVKPDWTIARIEHSKLLE TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC024945 |
| ORF Size | 546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC024945, AAH24945 |
| RefSeq Size | 839 bp |
| RefSeq ORF | 548 bp |
| Locus ID | 68115 |
| Gene Summary | Promotes sorting of SMDT1/EMRE in mitochondria by ensuring its maturation. Interacts with the transit peptide region of SMDT1/EMRE precursor protein in the mitochondrial matrix, leading to protect it against protein degradation by YME1L1, thereby ensuring SMDT1/EMRE maturation by the mitochondrial processing peptidase (PMPCA and PMPCB).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206919 | 9430016H08Rik (untagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472), (10ug) |
CNY 3230.00 |
|
| MR201651 | 9430016H08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472) |
CNY 2400.00 |
|
| MR201651L3 | Lenti ORF clone of 9430016H08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472) |
CNY 4750.00 |
|
| MR201651L4 | Lenti ORF clone of 9430016H08Rik (mGFP-tagged) - Mouse RIKEN cDNA 9430016H08 gene (cDNA clone MGC:28199 IMAGE:3989472) |
CNY 4750.00 |
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