1810054D07Rik (BC020085) Mouse Tagged ORF Clone
CAT＃: MG200536
- TrueORF®
1810054D07Rik (tGFP-tagged) - Mouse RIKEN cDNA 1810054D07 gene (cDNA clone MGC:28310 IMAGE:4013480)
ORF Plasmid: DDK
"BC020085" in other vectors (4)
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CNY 2850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RP23-17O21.2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG200536 representing BC020085
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAACTTGAAATATAATGTAAAGGGCACATCAGACTTTATCATCATGGAAGATAGACTTCACACTG AATGGTCCTCTCTGACCTGGACAGCCTTTCACATGGGGAACAATTTAAATCCATCCCAATCCATTTCTAA AACCTCATTGCTGCAGGAAGAAGCAGCAAGAAGAGCTCTTCTGTGTATTGAGGTGGCTGAGCAGGTTCTT AGCCTTAACCATGCAGAAGAAGGATTCGTGGATGCAGCCCCATCCCCTTCTAATTGCACTGTGTACAGTG ACAGAGTCAGCACAGTATTGCTGCGACAGGACTTTGTGTGCAAAGATCAAAGCAGCTGCCTCACTGTGTT TAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200536 representing BC020085
Red=Cloning site Green=Tags(s) MLNLKYNVKGTSDFIIMEDRLHTEWSSLTWTAFHMGNNLNPSQSISKTSLLQEEAARRALLCIEVAEQVL SLNHAEEGFVDAAPSPSNCTVYSDRVSTVLLRQDFVCKDQSSCLTVFK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC020085 |
| ORF Size | 354 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020085, AAH20085 |
| RefSeq Size | 641 bp |
| RefSeq ORF | 356 bp |
| Locus ID | 69863 |
| Gene Summary | Regulates high density lipoprotein (HDL) cholesterol metabolism by promoting the ubiquitination and degradation of the oxysterols receptors LXR (NR1H2 and NR1H3).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206935 | 1810054D07Rik (untagged) - Mouse RIKEN cDNA 1810054D07 gene (cDNA clone MGC:28310 IMAGE:4013480), (10ug) |
CNY 3230.00 |
|
| MR200536 | 1810054D07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810054D07 gene (cDNA clone MGC:28310 IMAGE:4013480) |
CNY 1200.00 |
|
| MR200536L3 | Lenti ORF clone of 1810054D07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810054D07 gene (cDNA clone MGC:28310 IMAGE:4013480) |
CNY 4750.00 |
|
| MR200536L4 | Lenti ORF clone of 1810054D07Rik (mGFP-tagged) - Mouse RIKEN cDNA 1810054D07 gene (cDNA clone MGC:28310 IMAGE:4013480) |
CNY 4750.00 |
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