Wnk2 (NM_001290313) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810073P09Rik; AW122246; ESTM15; mKIAA1760; X83337
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229705 representing NM_001290313 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCGATGGCGGCCGCCGAGACGCCCCCGGCGCGCTGATGGAAGCCGGGCGCGGCACGGGGTCGG CGGGCATGGCGGAGCCGCGGGCGAGGGCGGCGCGGCTCGGGCCCCAGCGCTTCCTGCGGCGCAGCGTGGT GGAGTCGGACCAGGAGGAGCCGCCGGGCCTGGAGGCGGCCGAGACGCCGAGCGCGCAGCCCCCACAGCCT CTGCAGCGCAGGGTGCTTCTACTCTGCAAGACGCGCCGGCTCATCGCGGAGCGCGCCCGGGGCCGCCCCG CCGCGCCCGCGCCCGCCGCGCCCGCTGCGCCTCCCGGCTCCCCGAGTGTCCCCTCGGACCCTGGCCCGGA GCGAGCGGGCACGCAGGAGCCCAGCCCAGATCCCACCACCGCCTCAGCCGCAGCCGCGCAGGTCCCCGAC GGCGGCCCGAGGCAGGAAGAGGCGCCGGCCCCAACACAGGAGGACGCTGGAACCACCGAGGCAAAGCCGG AGCCCGGGCGCGCACGCAAGGATGAACCTGAAGAGGAGGAAGACGATGAGGACGACCTCAAGGCCGTGGC CACCTCTTTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGGGGCTCCTTCAAGACGGTCTAC AAGGGGCTGGACACGGAGACCTGGGTGGAGGTAGCATGGTGTGAACTGCAGGACCGGAAGCTCACCAAGC TTGAACGGCAGAGATTCAAAGAGGAGGCGGAGATGCTGAAAGGTCTACAGCACCCCAACATTGTGCGCTT CTATGACTTCTGGGAGTCCAGTGCCAAGGGCAAGCGATGTATCGTGCTGGTGACAGAGCTGATGACCTCG GGTACACTGAAGACGTACCTGAAGCGGTTCAAGGTGATGAAGCCGAAGGTACTGAGGAGCTGGTGCCGGC AGATCCTGAAGGGCCTGCTGTTCCTGCATACAAGGACACCCCCCATCATCCACCGTGACCTCAAGTGTGA CAACATCTTCATCACTGGGCCCACCGGCTCTGTGAAGATTGGTGACTTGGGTCTGGCCACCCTTAAAAGA GCGTCTTTTGCCAAAAGTGTGATAGGTACCCCCGAGTTCATGGCACCTGAAATGTACGAGGAACATTACG ACGAGTCGGTTGATGTCTACGCCTTCGGGATGTGCATGCTGGAAATGGCTACCTCAGAGTACCCCTATTC GGAGTGCCAGAACGCCGCCCAGATCTATCGCAAGGTCACCTGTGGCATCAAACCGGCCAGCTTCGAGAAG GTGCATGATCCGGAGATTAAGGAGATCATCGGGGAGTGCATCTGTAAGAACAAAGAGGAGAGGTATGAGA TTAAGGACCTGCTGAGCCATGCCTTCTTCGCGGAAGACACGGGTGTCCGGGTGGAACTTGCCGAGGAGGA CCATGGCAGGAAGTCTACCATAGCCCTGCGGCTTTGGGTGGAAGACCCCAAGAAGCTGAAGGGCAAGCCC AAAGACAATGGAGCCATAGAGTTTACCTTTGACTTGGAGAAGGAGACTCCCGACGAGGTGGCCCAGGAAA TGATCGATTCTGGATTTTTCCATGAGAGTGATGTGAAGATTGTGGCCAAGTCCATCCGTGACCGGGTGGC ACTGATTCAGTGGCGGCGTGAGAGGATCTGGCCTGCACTGCAGTCTCAGGAGCCAAAGGACTCTGGCAGC CCTGACAAGGCCAGGGGTCTGCCAGCACCCCTGCAGGTCCAGGTGACCTACCACGCACAGAGTGGGCAGC CTGGGCAGCCAGAACCTGAGGAGCCTGAGGCCGACCAGCATCTCCTGCCCCCTACCTTGCCAGCTAGCGT TACCTCCTTAGCCTCGGACAGCACCTTTGACAGTGGCCAGGGCTCTACCGTGTACTCGGACTCCCAGAGC AGCCAGCAGAGCATGGTGCTCAGCTCGCTTGTGGACACGGCCCCAACCCCAGCCTCGTGCGTGTGCAGCC CCCCTGTGAGTGAGGGGCCTGGCCTGACCCACAGCCTGCCTACGCTAGGGGCCTTCCAGCAGCCGGCCAC TGTGCCTGGCCTGTCTGTGGGCCCTGTGCCACCCCCTGCCCGCCCTCCACTCCTCCAGCAGCATTTTCCA GAATCTTCCATGAGCTTCACCCCTGTGCTGCCACCACCCAGCACCCCTGTGCCCACGGGCCCAAGCCAGC CAGCACCCCCAGTCCAGCAGCCTCTTCCAATGGCCCAACCACCTACCCTGCCACAGGTCCTGGCCCCACA GCCCATGGGCACTGTCCAGCCAGTACCCTCTCATTTGCCTCCATACCTGGCTCCAACCTCCCAGGTCGTG GCCCCTGCTCAGCTGAAGCCTCTCCAGATGCCACAGCCACCTCTGCAACCTCTTGCTCAAGTCCCTCCGC AGATGCCTCAGATGCCTGTGGTCCCCCCCATCACCCCCTTAACAGGACTCGATGGCCTCCCTCAGACCCT CACTGACCTGCCGGCCGCAAACGTGGCCCCCGTGCCACCACCTCAGTATTTCTCACCTGCTGTGATCTTG CCAAGCCTCACAACACCCCTGCCCACATCACCGGCCCTGCCTATGCAGGCTGTCAAGTTGCCTCATCCCC CTGGAACACCCCTGGCTGTGCCCTGTCAGACCATCGTGCCAAATGCACCAGCTGCTATCCCCCTGTTGGC CGTGGCCCCGCAGGGTGTGGCTGCACTGTCTATCCATCCAGCGGTGGCCCAGATCCCAGCCCAGCCTGTG TACCCAGCAGCCTTTCCACAGATGGTACCTGGAGACATCCCACCCTCACCCCACCACACGGTGCAGAGCT TACGGGCCACCCCTCCACAGCTGGCCTCACCTGTGCCTCCTCAGCCTGTCCAGCCCAGTGTCATTCATCT ACCTGAGCAGGCTGCTCCAACTGCTGCCTCAGGGACCCAGGTCCTGCTGGGCCACCCTCCTTCATACACC GCGGACGTTGCTGCTCCAGTCTCTGCTGTGTCCCTGCCGCCTGCTGTCCTTTCCCCTCCTCTGCCAGATA CTCTGCTGCCTACTGTCCCTGACCTCCTGCCCAAGGTCCCCAGCTCTCTGGCCCCTACAGTGGTCGCAGC CTCTCAGAGTGCGCCCGCACAGACCTCTTCACTCCTCCTGCCAACAAACCCACCCCTGCCCACTGGGCCT GCAGTTGCTGGCCCCTGCCCTGCAGTCCAGCTGATGGTGGAAGTGGCTCAAGAGGAGCAGGTCTCACAAG ACAAACCCCCTGGCCCCCCACAGAGCTCCGAGAGCTTTGGAGGTTCTGATGTCACCTCTGGAAGAGATCT GAGTGACAGCTGTGAAGGCACCTTTGGAGGGGGCAGGCTGGAGGGAAGGACCGCCCGGAAACACCACCGT AGGTCCACTCGTGCACGCTCGCGGCAGGAGAGGGCCAGCCGGCCCCGGCTCACTATCCTAAATGTGTGTA ACACGGGGGACAAGATGGTGGAGTGTCAGCTGGAAACGCATAACCATAAGATGGTGACCTTCAAGTTTGA CCTCGATGGGGACGCTCCGGATGAGATTGCCACGTACATGGTAGAGCATGACTTTATCCTGCCCGCTGAG CGAGAGACCTTCATCGAGCAGATGAAGGATGTCATGGACAAGGCAGAAGACATGCTCAGTGAGGACACAG ATGCTGACCATGGCTCTGATACAGGGACCAGCCCTCCACACCTGGGTACCTGTGGTCTGGCCACGGGGGA GGAGAACAGACAGTCCCAAGCCAATGCCCCTGTCTACCAGCAGAATGTCTTACACACGGGAAAGAGGTGG TTTATCATCTGTCCAGTGGCTGAACACCCAGCAACAGACACTTCTGAATCATCACCCCCACTTCCTCTAA GCTCCTTGCAGCCAGAAGCCAGCCAAGACCCAGCGCCCTATCCAGACCAGCTGTCCTTGACGGACAAACC TAGTTTCCCAGCTGCTCAGCAGCTCCTGAGCCAGGCAGGCTCCAGCAACCCTCCTGGTGGAGCATCAGCC CCCTTGGCCCCGTCTTCCCCTCCTGTGACTACTGTAATCCCGGCAGCACCAGCCACCAGCACCGTGCCAG AGTCAGCAGCAGGGACTGCCATGCAGGCAGGAGGTCCAGGGACTCATCAAGGACCAGCCAGTGTCCATGA GACCCTCCAGCCACTGGCCGAGACACGCTCTGCCCAGTGTACTGCCCAGCCCCTTAGTACTGGCCAAGGA CCTTGCACCCCAGCTCTGGAAGCCTCTCGCTGCTCCACAGGTCTGGGGGAGCCCATCTCAACCAGGGAGG TCAGCACTCAAGGAGAGCCCCTGCCTGCTTCAGTGCCAGAACCTAGCCCTCCCACTGGGGCCACACAATC TGTACCGGGTCAGCCACCACCCCCACTGCCCATCACAGTAGGGGCTATCAGTCTGGCAGCTCCCCAGCTC CCCAGTCCCCCCCTGGGACCTACTGCTCCCCCACCACCCCCCTCAGCCTTGGAATCTGATGGGGAAGGGC CGCCCCCTAGGGTGGGCTTCGTGGACAACACCATCAAGAGTCTGGATGAGAAACTGCGGACTCTGCTCTA CCAGGAGCACGTGCCCACCTCCTCAGCCTCAGCTGGGACCCCCATGGAGGCAAGCGATAGAGACTTCACC CTGGAGCCCCTGCGAGGAGACCTTCCCTCAGCCTTGAGTGACAAGACCCCAAGCCTCACCCAGCAGACCC AGCCCTCATTGGAAAAGTCTGAAACGGCCCCTGCAGGATGGGCCCTGGCCCAGAGGGAACAGGGTGCATC TTCTCCAATGACAGCAGAGTCATCTTCCAGCAATACACTGGGCTGTGACAGTGACGCAGGGCAGGTGGCC TCGGACTCCTCCACAGCACCCAGTGTCCCCCAGGATGCCTCTGGTTCTTCCGTCCCTACACACATGGATC CCAAAGATCAGAACAGTAGTGTTCCCAGAGAAGCCTTGGCAGCACCCATGCAAAGTGGCCCAGGGTCCTT CACAGTGGGCAGCCCAGCCCAGCTGCGTGGCGCCCGGGACTCCGGGTCTCCTCATAAGCGGCCAGGGCAG CAGGACAACAGTTCTCCAGCCAAAACTGTAGGGCGCTTTTCCGTGGTCAGCACACAGGACGAGTGGACTC TGGCGTCACCTCACAGCCTGCGGTACTCTGCCCCACCCGATGTCTACCTGGATGAGATCCCCTCTAGCCC TGAGGTGAAGCTGGCAGTGCGGCGGGTACAGACAGCCTCCTCCATCGAGGTTGGCGTTGAGGAACCTGCC TCTAGTGACTCTGGGGATGAACGCCCAAGAAGGAGGTCCCAGGTGCAGAAGCAGTCCTCCCTGCCGGGTA CTGGTGGTGTGGCAAGTGACTTTGTGAAGAAGGCCACTGCCTTCCTGCACAGGTCTTCAAGGGCTGGCTC GCTGGGACCTGAGACACCCAGTAGGGCAGGTGTGAAGGTCCCTACCATCAGCATCACCTCCTTCCACTCC CAGTCATCCTACATCAGCAGTGACAATGACTCAGAGTTTGAGGATGCAGACATAAAGAAGGAACTGCGCA GTCTGCGAGAAAAGCACCTGAAGGAAATCTCGGAGCTACAGAGCCAGCAGAAGCAGGAGATCGAAGCCCT GTACCGACGCTTGGGCAAGCCGCTCCCTCCCAATGTGGGGTTCTTCCACACTGCACCCCCTATGGGCCGC AGGAGGAAAACCAGCAAGAGCAAATTGAAGGCTGGCAAGCTGCTGAACCCCTTGGTGCAACAGCTCAAGG TCGTGGCCTCCAGCACAGGTCATTTGTCTGACTCCAGCAGAGGCCCTCCCACTAAGGACCCTGCCCACGC CAGCACGCCCCCGGGCACCAAGGCAGTTCAGACCCAGCAGCCCTGCTCCGTTCGGGCCTCTCTGTCCACA GACATCTGCTCCGGTTTAGCCAGTGATGGAGGCGGAGCGCGTGGCCAAGGCTGGACGGTTTACCACCCAA CGTCTGAGAGAGGGGCCTATAAGTCTAGTAGCAAGCCTCGTGCTCGATTCCTCAGTGGACCCGTATCTGT GTCCATCTGGTCAGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAGCAGTAGGTCTTCCACCAGCAGC CTGGCCCCAGGCCCTGAGCCAGGTCCCCAGCCCACCCTGCACGTCCAGGCGCAGGTGAACAACAGCAACA ACAAGAAAGGCACCTTCACGGACGACCTGCACAAGTTGGTGGACGAGTGGACAACGAAGACCGTGGGGGC AGCGCAAGTGAAGCCCACGCTCAACCAGCTGAAGCAGACGCAGAAGCTGCACGACATGGAGGCCTCTGGA GACGCTCGGGCTACATCTGTGCCTCGAGCAGCAGTGGGGGCATCGTGTCTGGCCCCGGCCCCTGGCCCTC TGTCCACCACAGCCACTCCTGGAGCCACTCCAGCCCTGCCTGTGCCCATACCAGATCCTGAGAGTGAAAA GCCCGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290313
Insert Size	6450 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290313.1, NP_001277242.1
RefSeq Size	7202 bp
RefSeq ORF	6450 bp
Locus ID	75607
UniProt ID	Q3UH66
Gene Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains alternate 3' exon structure, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. This variant is partially supported by the mRNA BC060187.1; the 5' exon structure is inferred based on other transcript alignments at this gene. CCDS Note: This CCDS representation is partially supported by the mRNA BC060187.1. The 5' exon structure is inferred based on other transcript alignments at this gene.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.