Snapc4 (NM_001290419) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730436L13Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229499 representing NM_001290419 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTACCAGGAGGTGATCCGGGAAAAGCTAGCAGAGGTTAGCCAGTTGCTAGCCCAGAACCAGGAGC AGCAGGAAGAGATCCTGTTTGATCTGTCTGGAACCAAATGCCCCAAGGTGAAGGATGGCAGAAGTCTGCC CTCATACATGTACATAGGCCACTTCCTGAAGCCATACTTCAAGGACAAGGTTACTGGAGTGGGGCCTCCT GCCAACGAAGAAACACGGGAGAAGGCTACCCAGGGAATCAAGGCCTTTGAACAGCTCTTGGTGACCAAGT GGAAGCACTGGGAGAAAGCCTTGCTCAGGAAGTCTGTAGTGAGTGACCGCCTACAACGTCTGCTTCAGCC CAAATTGCTGAAGCTGGAGTACTTACACGAAAAACAGAGCCGAGTCTCCAGTGAGTTGGAAAGGCAGGCC CTGGAGAAGCAGATTAAGGAAGCAGAGAAGGAGATTCAGGACATCAACCAGCTCCCAGAGGAAGCATTGT TGGGGAACCGGCTAGACAGCCACGACTGGGAGAAGATTTCCAATATTAACTTTGAAGGAGCCCGCAGTGC AGAGGAGATCCGGAAGTTCTGGCAGAGTTCTGAGCATCCAAGCATCAGTAAGCAGGAATGGAGCACAGAA GAAGTAGAGAGGCTGAAGGCCATCGCTGCCACACATGGCCACCTGGAGTGGCACTTGGTTGCAGAGGAAC TTGGGACAAGCCGCAGTGCCTTTCAGTGCCTGCAGAAATTCCAGCAGTACAACAAAACCCTGAAACGCAA GGAGTGGACAGAAGAGGAAGACCACATGCTCACCCAGCTGGTCCAAGAGATGCGAGTTGGGAACCATATC CCATACCGCAAGATCGTCTACTTCATGGAAGGGAGAGACTCCATGCAGCTGATCTACCGTTGGACCAAGA GCTTAGATCCCAGCCTGAAAAGGGGATTCTGGGCCCCAGAGGAAGATGCTAAATTGCTTCAAGCTGTTGC CAAGTATGGGGCGCAGGACTGGTTTAAAATCCGGGAAGAGGTGCCAGGTAGGAGCGATGCCCAATGCAGA GACCGCTACATCAGAAGATTACATTTCAGCTTGAAAAAGGGACGGTGGAATGCAAAAGAAGAACAACAGC TGATCCAGCTAATAGAGAAATATGGTGTTGGTCACTGGGCAAGAATCGCTTCTGAATTGCCCCATCGGTC AGGCTCCCAGTGTCTGAGCAAGTGGAAGATCTTGGCCAGGAAGAAACAGCATCTGCAGAGGAAGAGAGGG CAGAGGCCCCGTCACAGTAGCCAGTGGAGCTCTAGCGGCAGCAGCAGCAGCAGCAGTGAAGACTACGGCA GCAGCAGTGGCAGCGACGGCAGCAGCGGCAGTGAAAACTCAGATGTGGAGCTGGAGGCGTCTCTGGAGAA GAGTAGAGCACTGACTCCTCAACAGTACAGAGTGCCAGACATTGACCTGTGGGTTCCTACCAGGCTGATC ACCAGCCAGTCACAGAGAGAAGGGACAGGGTGCTACCCACAACACCCTGCTGTCTCCTGCTGCACTCAGG ATGCCTCTCAAAATCACCACAAGGAAGGTTCTACCACCGTCTCTGCTGCTGAGAAAAACCAGCTGCAGGT GCCCTATGAGACCCACAGCACTGTTCCAAGAGGAGACCGGTTCCTACACTTCTCGGACACCCACTCAGCA AGTTTGAAGGATCCGGCCTGCAAGAGTCACACACTGATGAAGGAAAGACCGAAGCAGCCACTCCTCCCTA GTTCACGCTCAGGGTCTGACCCTGGCAACAATACGGCTGGGCCCCATCTGCGGCAGCTATGGCATGGAAC TTACCAGAATAAGCAGAGACGCAAGAGACAGGCTCTACATCGCAGGCTCCTTAAGCACAGGCTTTTGCTG GCTGTGATACCCTGGGTGGGTGACATCAACCTGGCCTGCACACAAGCTCCCCGGAGACCTGCAACCGTTC AGACTAAAGCTGACAGCATCCGGATGCAGTTAGAGTGTGCCCGCCTGGCCAGCACTCCGGTATTCACACT GCTTATTCAGCTATTGCAGATTGATACTGCGGGCTGCATGGAGGTGGTTCGAGAGAGGAAAAGCCAGCCC CCTGCACTGCTCCAGCCTGGCACCCGGAACACCCAGCCGCATCTCCTGCAGGCATCCTCTAATGCCAAGA ACAACACAGGCTGCCTCCCAAGCATGACGGGGGAACAAACTGCAAAGAGAGCCAGCCACAAAGGGCGCCC ACGACTGGGGTCATGCAGGACTGAGGCTACCCCCTTCCAAGTCCCTGTAGCAGCCCCACGTGGCCTCCGG CCAAAGCCTAAGACTGTTTCTGAGCTGCTTCGGGAGAAGCGGCTCCGTGAGTCCCATGCCAAGAAGGCCA CTCAGGCTCTTGGTCTCAACAGCCAGCTGCTGGTCTCCTCACCTGTGATCCTCCAGCCCCCTCTGCTGCC AGTCCCCCATGGTTCCCCAGTAGTAGGCCCTGCAACGTCCAGTGTAGAACTCTCTGTACCTGTGGCGCCG GTGATGGTCAGTTCAAGTCCTTCTGGCTCCTGGCCGGTGGGTGGGATCTCAGCCACAGACAAGCAGCCCC CCAACCTACAGACCATTTCCCTAAATCCTCCCCACAAAGGGACCCAGGTTGCAGCCCCTGCTGCTTTTAG GAGCCTAGCCTTAGCCCCCGGGCAAGTCCCCACAGGCGGCCATCTGAGTACTCTAGGACAGACCTCTACC ACATCCCAGAAGCAGAGCCTGCCCAAGGTCCTGCCCATTCTTCGAGCTGCTCCCAGCCTCACTCAGCTGT CTGTGCAGCCACCAGTAAGTGGACAGCCCCTGGCAACCAAGTCCAGCCTGCCTGTCAACTGGGTTCTCAC AACTCAGAAGTTGCTCTCTGTCCAAGTGCCAGCTGTGGTGGGCCTCCCCCAGTCAGTGATGACCCCTGAG ACCATAGGACTACAAGCAAAACAGCTGCCTTCCCCTGCCAAGACTCCTGCTTTTCTGGAACAGCCTCCAG CCAGTACAGACACAGAACCCAAGGGACCTCAGGGCCAGGAAATACCACCCACACCTGGGCCCGAAAAGGC AGCTTTGGACCTAAGTCTCCTTTCCCAGGAAAGTGAGGCAGCCATCGTCACATGGCTGAAGGGGTGCCAA GGTGCTTTTGTACCCCCGCTGGGGAGCAGGATGCCCTATCATCCCCCTTCCCTGTGCAGCTTGCGGGCAT TGTCCAGCCTCCTCCTCCAGAAACAGGACTTGGAGCAGAAGGCTTCCTCTCTGGCGGCCAGCCAGGCAGC TGGGGCCCAACCTGATCCCAAGGCTGGAGCCCTACAAGCTTCCCTGGAGCTGGTACAGAGGCAATTCCGG GACAACCCAGCCTACCTCCTGCTAAAGACGCGTTTCCTGGCTATCTTCTCCCTTCCTGCATTCCTGGCCA CTCTGCCCCCCAACAGCATCCCCACCACCCTGTCACCAGACGTGGCTGTGGTTTCTGAGAGTGACAGTGA AGACCTCGGTGACTTGGAGCTCAAGGATAGGGCTAGACAGTTGGACTGCATGGCCTGCAGAGTGCAAGCA AGCCCAGCAGCCCCAGACCCTGTACAGAGAGCTCCAAGTCCTGGTGAGGTTTCTGCTCCCTCCCCTCTGG ATGCTTCTGATGGCCTTGATGACCTTAACGTGCTCAGGACTCGACGTGCTCGGCATTCCCGGAGATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290419
Insert Size	3708 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290419.1, NP_001277348.1
RefSeq Size	4507 bp
RefSeq ORF	3708 bp
Locus ID	227644
UniProt ID	Q8BP86
Gene Summary	Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) includes an additional exon in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

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