Atp2b2 (NM_009723) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D6Abb2e; dfw; Gena300; jog; PMCA2; Tmy; wms; wri
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229464 representing NM_009723 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT TCGGGTGCACCATGGAGGAGCTGCGCTCCCTCATGGAGCTGCGGGGCACCGAGGCTGTGGTCAAGATCAA GGAGACGTATGGGGACACTGAAGCCATCTGCCGGCGCCTCAAAACCTCGCCTGTTGAAGGTTTACCAGGC ACTGCTCCAGACTTGGAAAAGAGGAAACAGATTTTTGGGCAAAACTTCATACCTCCAAAGAAACCAAAAA CCTTCCTGCAGCTGGTGTGGGAAGCGCTACAGGACGTGACACTTATCATCCTGGAGATCGCGGCCATCAT CTCCCTGGGACTGTCCTTCTACCACCCACCGGGAGAGAGCAATGAAGGATGTGCCACGGCCCAGGGTGGG GCAGAGGATGAAGGTGAAGCAGAAGCAGGCTGGATTGAGGGGGCTGCCATCCTGCTGTCAGTCATCTGTG TGGTCCTGGTCACAGCCTTCAATGACTGGAGTAAGGAGAAACAGTTCCGGGGCCTGCAGAGCCGAATTGA GCAGGAGCAGAAGTTCACTGTGGTCCGGGCCGGCCAGGTGGTTCAGATCCCTGTGGCCGAGATCGTGGTC GGGGACATTGCCCAGATCAAATATGGTGACCTTCTTCCCGCTGATGGCCTCTTCATCCAGGGAAATGACC TCAAAATTGATGAAAGCTCACTCACAGGGGAATCTGACCAGGTGCGCAAGTCTGTGGATAAGGACCCCAT GTTGCTGTCAGGAACCCATGTGATGGAGGGCTCAGGACGGATGGTGGTTACTGCTGTGGGTGTGAACTCT CAGACTGGCATCATATTTACCCTGCTTGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGCCA AGCAGCAGGATGGGGCAGCTGCCATGGAGATGCAGCCTCTGAAGAGTGCAGAGGGCGGCGATGCAGATGA CAAGAAGAAAGCCAACATGCACAAGAAAGAGAAGTCGGTGCTTCAGGGCAAGCTCACCAAACTGGCTGTG CAGATAGGCAAGGCGGGCCTGGTGATGTCGGCCATCACAGTGATCATCCTGGTACTCTACTTCACCGTGG ACACCTTCGTGGTCAACAAGAAGCCATGGCTGACGGAATGCACACCCGTCTACGTACAGTACTTTGTCAA GTTCTTCATCATTGGTGTGACGGTGCTGGTGGTCGCTGTGCCCGAGGGCCTCCCTCTGGCTGTCACCATC TCACTGGCCTATTCTGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGA CCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGAACGCTGACCACCAACCGCATGACCGTGGT CCAGGCCTATGTCGGTGACGTCCACTACAAGGAGATCCCCGATCCCAGCTCCATCAATGCCAAGACGCTG GAGCTGCTGGTCAACGCCATTGCCATCAACAGCGCCTACACCACCAAGATCCTTCCCCCAGAAAAAGAGG GAGCCCTGCCCCGGCAGGTGGGCAACAAGACAGAGTGCGGCCTGCTGGGCTTTGTGCTGGACTTGAGGCA GGACTACGAGCCGGTGCGCAGCCAGATGCCAGAGGAGAAGCTGTATAAGGTGTACACCTTCAACTCCGTG CGCAAGTCCATGAGCACCGTCATCAAGATGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGCGCCTCGG AGATTGTGCTCAAAAAGTGCTGCAAGATCCTCAGTGGGGCAGGGGAAGCCCGTGTCTTCCGGCCCCGAGA CAGGGATGAGATGGTTAAGAAGGTGATTGAGCCCATGGCCTGTGACGGGCTCCGTACCATCTGCGTGGCC TATCGTGACTTCCCCAGCAGCCCTGAGCCTGACTGGGACAATGAGAATGACATTCTCAATGAACTCACGT GCATCTGCGTGGTGGGCATCGAAGACCCAGTACGACCTGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCG GGCAGGTATCACAGTCCGCATGGTCACCGGTGACAATATCAACACAGCCCGGGCCATCGCCATCAAGTGT GGCATTATCCACCCTGGAGAGGACTTCCTGTGCCTGGAAGGCAAAGAATTCAATCGGAGGATTCGCAACG AGAAGGGGGAGATTGAGCAGGAGCGGATTGACAAGATCTGGCCAAAGCTGAGGGTGCTGGCTCGCTCCTC GCCCACGGATAAGCACACGCTGGTCAAAGGCATCATCGACAGTACACACACTGAGCAGCGGCAGGTGGTG GCTGTGACAGGGGATGGGACCAACGACGGGCCTGCTCTCAAGAAGGCAGATGTGGGCTTCGCAATGGGCA TCGCAGGCACAGATGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGATGACAACTTCAGCAGCATCGT CAAGGCAGTGATGTGGGGCCGTAACGTCTATGACAGCATATCCAAATTCCTGCAGTTCCAGCTGACTGTC AACGTGGTGGCGGTGATCGTGGCCTTCACGGGCGCCTGCATTACACAGGACTCCCCTCTCAAGGCTGTGC AGATGCTCTGGGTGAACCTCATCATGGACACGTTTGCCTCCCTGGCCCTGGCCACAGAGCCACCTACGGA GACTCTGCTTCTGAGGAAACCGTACGGTCGCAACAAGCCGCTCATCTCGAGGACCATGATGAAGAACATC CTGGGCCACGCCGTCTACCAGCTCACCCTCATCTTCACCCTGCTCTTCGTGGGTGAGAAGATGTTCCAGA TCGACAGCGGAAGGAACGCCCCGCTGCACTCACCACCCTCAGAGCACTACACCATCATCTTCAACACCTT CGTCATGATGCAGCTTTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAACGTCTTTGACGGG ATCTTCCGGAACCCCATCTTCTGCACCATCGTTCTTGGCACTTTCGCCATCCAGATAGTGATCGTGCAGT TTGGCGGGAAGCCCTTCAGCTGCTCCCCACTCCAGCTGGACCAGTGGATGTGGTGCATCTTCATAGGCCT GGGAGAGCTCGTCTGGGGCCAGGTCATCGCCACCATCCCTACCAGCAGGCTCAAGTTCCTGAAAGAGGCA GGGCGTCTAACACAGAAGGAGGAGATCCCCGAGGAGGAGTTAAATGAGGATGTGGAAGAGATAGACCACG CAGAGCGGGAGCTTCGCCGTGGCCAGATCCTGTGGTTCCGGGGCCTGAATCGGATCCAGACACAGATCCG CGTCGTGAAGGCGTTCCGTAGCTCTCTCTATGAAGGGTTAGAAAAACCCGAGTCTCGAACCTCCATCCAT AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATCGATGACACCG ACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCCTCGCTCAACAAGAACAATAGCGC CATCGACAGCGGGATCAACCTGACGACCGACACGAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCC ATCCACAGCCTGGAGACGTCGCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009723
Insert Size	3597 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009723.5, NP_033853.1
RefSeq Size	7111 bp
RefSeq ORF	3597 bp
Locus ID	11941
UniProt ID	Q9R0K7
Gene Summary	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR and coding region, compared to variant 3. These differences result in a shorter protein (isoform 1), compared to isoform 2. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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