Atp13a5 (NM_001284375) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C630015F21Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229438 representing NM_001284375 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGAGCAAGAAGGATGGCCACCAGGCTGTGCTCAATGAAGGGGAGGAGAATGAGCTGGAGGTGT TTGGCTACCATACCCAGAATTTACGGAGAGCACTGTGCCTTGTCACAGCCATCCTGACTCTTGGGGCAGT TCAGCTGATGTTCTACTGGAGGCCTGAGTGGTGGGTGTGGACCAGTTGCATCCCATGCCCCTTGCAAGAA GCAGACACAATTTTGCTGAGGACAACCGATGAATTTCGAAGATATATGAGGAAAAAGGTCTTCTGCCTCC ACTTATCCACACTGAAGTTTCCTATAAGCAAGAACCCAGAGGAACCTCTGGTAGCTGACCACCACTCAGT CATAAACCAAGCTGTGATGAAGCCAGAACTAAAACTACGATGCATCCAAGTGCAGAAAATAAGGTACGTG TGGGATTTCTTGAAGAAGCGATTTCAGAAAGTTGGGTTGCTGGAAGACAGTAACTCCTGCTTTGACATCC ACCATACGTTTGGATTGGGTCTGACCAATGAAGAGCAAGAGGTCAGAAGGTTAGTGTGTGGGCCCAACTC GATCGAGGTGGAGATCCAACCCATATGGAAGCTGCTTGTTAAACAGCAATCAGTTAAGCTACACAAGCTT GTGGAGGAGCACAACAAAGTCCAGGTCACGATCACAGTAAGAGACAAAGGTCTACAGGAGCTGGAGTCCC GTCTCCTGGTTCCAGGAGATATTCTCATTCTTCCAGGGAAAATTTCACTGCCCTGTGATGCTATCTTGAT CGATGGGAGTTGTGTGGTGAATGAAGGAATGCTCACAGGAGAAAGTATACCTGTTACAAAGACACCATTG CCCCAAACGGAGAACACCATGCCCTGGAAATCGCACAGTTTGGAGGACTACCGGAAACACGTGCTCTTCT GTGGAACAGAAGTTATTCAGGTCAAGCCATCTGCTCAGGGGCTTGTTAGAGCTGTTGTTCTGCAGACAGG TTATAATACAGCCAAAGGAGACTTAGTGAGATCCATCCTCTACCCTCGACCTCTGAACTTCAAACTGTAT AATGATGCCTTCAAGTTCATGGTGTTCCTGGCCTGCGTTGGTGTTGTGGGATTTTTCTATGCCCTAGGTG TCTATATGTACCATGAAGTACCTCCGAGAGAAACTGCAACCATGGCCCTGATCCTTCTCAGTGCCACCGT CCCTCCCGTGCTCCCGGCTGCACTGACCATAGGCAACGTGTACGCCCAGAAGAGGTTGAAGAAGGAGAAG ATTTTCTGTATCTCCCCGCAGAGAATCAACATGTGTGGTCAGATCAACCTCGTGTGCTTCGACAAAACAG GAACTCTGACAGAAGATGGGCTGGACCTCTGGGGGACTGTCCCCACCGCTGGCAACTGTTTCCAGGCAGT CCACAGCTTTGCCTCAGGTGAGGCTGTGCCCTGGGGCCCACTGTGTGCAGCCATGACTAGCTGCCATTCT CTGATCCTTCTTGATGGGACCATCCAAGGAGACCCTTTGGACCTCAAAATGTTTGAAGGCACTGGTTGGA ATATGGAAGATAGCCAAGTGGCCTCCTGCAAATTTGGCATGGCAGATTCAAGCACAGTAATAAAACCAGG ACCGAAAGCCAGCCAGAGTCCTGTGGACAGCATCACCATCTTGCGCCAGTTTCCATTTTCCTCAGGCCTG CAAAGGATGTCTGTGATTGCTCAGCTGGCTGGTGACCTTCACCTCCACGTCTACATGAAGGGAGCCCCAG AAATGGTGGCCAGGTTCTGCCGCTCTGAAACGGTGCCTAAGAACTTCTCGCAGGAGCTGAGGAATTACAC AGTGCAAGGCTTCCGAGTCATTGCTCTTGCCCACAAAACCCTAAAGATGGAGAGGCTTTCAGATATGGAC CATCTAGCAAGGGAGAAAGTGGAGTCTGAGTTGGCCTTTCTGGGACTTCTCATAATGGAGAATCGCTTGA AAAAGGAAACAAGGCCAGTGCTGAAGGAGCTGAGTGAGGCCCGCATCAGGACCGTGATGGTTACAGGTGA CAATCTTCAAACTGCCATCACTGTTGCTAAAAATTCTGAAATGATTCCTGTGGGCAGCCAGGTCGTCATT GTCGAGGCTAATGAGCCAGGAGACCTTGTTCCTGCTTCTGTGACCTGGCAGCTGGTGGGGACCCAAGAGC CAGGCTCTGGGAAGAAGGACACCTACATTGACATCGGAAACAGCTCTGTACCTGCTGGGAAAGGTTACCA TTTTGCAATGAGTGGGAAGTCATACCAAGTGTTATTTCATCATTTCTACAGCATGCTGCCACAAATTCTG GTTAATGGAACCATTTTTGCAAGAATGTCTCCTGGGCAGAAGTCAAGCCTCGTGGAAGAGTTTCAGAAAT TAAATTATTATGTGGGCATGTGTGGAGATGGAGCCAATGACTGTGGGGCTTTGAAAATGGCTCATGCTGG CATCTCACTATCAGAACAGGAAGCATCCGTGGCATCTCCCTTCACCTCGAAAACAGCCAATATCGAGTGT GTGCCTCATCTCATCAGGGAGGGCCGGGCTGCTCTGGTCTCATCCTTCGGTGTATTTAAATACCTGACTA TGTACGGCATCATCCAGTTTATTGGAACATCACTTCTGTACTGGCAACTACAGCTCTTTGGTAACTACCA GTACCTCCTGCAAGATGTGGCCATCACGCTGATGGTCAGCTTAACAATGAGCATAAACCATGCCTACCCG AAGTTGGCTCCCTACAGACCAGCAGGACAACTACTTTCTCCTCAGTTGCTACTCTCAGTCTTCATGAATT CCTGTTTTACCTGCATTGTGCAAGTGTGTACATTTCTCACCGTGAAACAGCAGCCTTGGTACTGCGAGGT CTACAAATACAGTGAATGCTTTCTGGTCAACCAAAGTAACCTCTCAGCCAACGTGAGTTTGGACCGAAAC TGGACTGGAAATGCAACTCTGGTTCCCGCTTCTGTGCTAAGTTTCGAGGGCACTACGTTGTGGCCCATCG TCACCTTCAACTGCATCTCTGCAGCATTTATTTTTTCTAAGGGAAAGCCGTTTCGGAAACCCATCTACGC AAACTATTTATTCTCCCTTCTGCTGGCATCTGCTGCGGGCCTCACTATTTTCATTCTGTTTTGTGATTTT CAAGACCTGTACCGTAAAATGGAGTTCATCCCAACCCCCACATCATGGAGAGTTTCAATTCTGATAGCAG CTTTTGTCCAGTTCTGCGTTGCCTTCTTTGTAGAGGATGCCGTCCTTCAAAACAGAGAGCTCTGGCTGTT TATCAAGAAAGAATTTGGATTCTACTCCAAAAGTCAGTATAGGATCTTGCAGAGAAAGCTGGCAGAGGAC TCTACATGGCCTCCCGTGAACAGGACAGATTATGCAGTCAATGGCAAAAATGGATTCTATGTCAACAGAG CCTATGAGAGCCCCGAAGAGGTCCCCAAAGGGAAGCTCAAGCTGGAAGAGCAGGCTTCAGAACAGCACTT TTGGACTAGACTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284375
Insert Size	3516 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284375.1, NP_001271304.1
RefSeq Size	4400 bp
RefSeq ORF	3516 bp
Locus ID	268878
UniProt ID	Q3TYU2

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