Ptprc (NM_001268286) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; T200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229399 representing NM_001268286 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGGTTTGTGGCTCAAACTTCTGGCCTTTGGATTTGCCCTTCTGGACACAGAAGTCTTTGTCA CAGGGCAAACACCTACACCCAGTGATGCTACCACAACGAAGCAAACATGTGCTGCCATGTTTGGGAACAT TACTGTGAATTACACCTATGAATCTAGTAATCAGACTTTTAAGGCAGACCTCAAAGATGTCCAAAATGCT AAGTGTGGAAATGAGGATTGTGAAAACGTGTTAAATAATCTAGAAGAATGCTCACAGATAAAAAACATCA GTGTGTCTAATGACTCATGTGCTCCAGCTACAACTATAGATTTATATGTACCACCAGGGACTGACAAGTT TTCGCTACATGACTGCACACCAAAAGAAAAGGCTAATACTTCAATTTGTTTGGAGTGGAAAACAAAAAAC CTTGATTTCAGAAAATGCAACAGTGACAATATTTCATATGTACTCCACTGTGAGCCAGAAAATAATACAA AATGCATTAGAAGAAATACATTCATACCTGAAAGATGTCAGTTGGACAACCTTCGTGCCCAAACAAATTA CACATGTGTAGCAGAAATCTTATATCGCGGTGTAAAACTCGTCAAAAATGTTATAAATGTGCAGACAGAT TTGGGGATTCCAGAAACGCCTAAGCCTAGTTGTGGGGATCCAGCTGCAAGAAAAACGTTAGTCTCTTGGC CTGAGCCTGTATCTAAACCTGAGTCTGCATCTAAACCCCATGGATATGTTTTATGCTATAAGAACAATTC AGAAAAATGTAAAAGTTTGCCTAATAATGTGACCAGTTTTGAGGTGGAAAGCTTGAAACCTTATAAATAC TATGAAGTGTCCCTACTTGCCTATGTCAATGGGAAGATTCAAAGAAATGGGACTGCTGAGAAGTGCAATT TTCACACAAAAGCAGATCGTCCGGACAAGGTCAATGGAATGAAAACCTCCCGGCCGACAGACAATAGTAT AAATGTTACATGTGGTCCTCCTTATGAAACTAATGGCCCTAAAACCTTTTACATTTTGGTAGTCAGAAGT GGAGGTTCTTTTGTTACAAAATACAACAAGACAAACTGTCAGTTTTATGTAGATAATCTCTACTATTCAA CTGACTATGAGTTTCTGGTCTCTTTTCACAATGGAGTGTACGAGGGAGATTCAGTTATAAGAAATGAGTC AACAAATTTTAATGCTAAAGCACTGATTATATTCCTGGTGTTTCTGATTATTGTGACATCAATAGCCTTG CTTGTTGTTTTGTATAAAATCTATGATCTGCGCAAGAAAAGATCCAGCAATTTAGATGAACAACAGGAAC TCGTTGAAAGGGATGATGAAAAGCAGCTGATGGATGTGGAGCCAATCCATTCTGACATTTTGTTGGAAAC ATACAAAAGGAAGATTGCTGATGAGGGCAGACTGTTCCTGGCTGAATTTCAGAGCATTCCACGGGTATTC AGCAAGTTTCCCATCAAAGATGCCCGAAAGCCCCACAATCAGAATAAAAACCGTTATGTTGACATTCTTC CCTATGATTATAACCGTGTGGAACTCTCTGAAATAAATGGAGATGCAGGGTCCACCTACATAAATGCCAG CTACATTGATGGCTTCAAGGAACCCAGGAAATACATTGCTGCACAAGGGCCCCGGGATGAGACAGTTGAT GACTTCTGGAGGATGATCTGGGAGCAAAAGGCCACAGTTATTGTCATGGTCACACGATGTGAAGAAGGAA ACAGGAACAAGTGCGCAGAATACTGGCCAAGCATGGAGGAAGGCACTCGGGCTTTCAAAGATATTGTTGT GACAATCAATGACCACAAACGATGTCCTGATTACATCATTCAGAAGCTGAACGTTGCACATAAAAAAGAA AAAGCAACTGGAAGAGAAGTGACTCATATCCAATTCACCAGCTGGCCAGACCATGGGGTTCCTGAAGACC CTCACCTGCTCCTCAAACTTCGACGGAGAGTTAATGCTTTTAGCAACTTCTTCAGTGGTCCCATTGTGGT GCACTGCAGTGCTGGTGTTGGGCGTACAGGTACCTACATTGGAATTGATGCCATGCTGGAAGGCCTGGAA GCAGAGGGCAAAGTGGATGTCTATGGTTATGTTGTCAAGCTAAGGCGACAGAGGTGTCTGATGGTGCAAG TGGAGGCACAGTATATCCTGATTCATCAGGCTTTAGTGGAATACAATCAGTTTGGAGAAACAGAAGTGAA CTTGTCTGAGTTACATTCATGCCTACACAACATGAAGAAGAGAGATCCACCCAGTGACCCCTCCCCTCTG GAGGCTGAATACCAGAGACTTCCTTCATACAGGAGTTGGAGGACACAGCACATTGGAAATCAAGAAGAAA ATAAGAAGAAGAACAGGAATTCTAATGTTGTTCCATATGACTTTAACAGAGTGCCACTTAAGCATGAACT GGAGATGAGCAAAGAGAGTGAGCCTGAATCAGATGAGTCTTCAGATGATGACAGTGACTCAGAAGAAACC AGCAAATACATTAATGCATCCTTTGTGATGAGTTACTGGAAACCAGAAATGATGATTGCTGCTCAGGGGC CACTAAAAGAAACGATCGGTGACTTTTGGCAGATGATATTCCAAAGAAAAGTCAAAGTTATTGTGATGTT GACAGAGTTAGTGAATGGAGACCAGGAAGTCTGTGCTCAGTACTGGGGCGAAGGAAAGCAGACTTATGGA GACATGGAAGTGGAGATGAAAGACACAAACAGAGCCTCAGCCTACACTCTCCGAACTTTTGAGCTGAGAC ATTCCAAGAGGAAGGAGCCCAGAACTGTGTACCAGTACCAGTGTACCACATGGAAAGGGGAAGAGCTGCC TGCAGAACCCAAAGACCTGGTGTCTATGATTCAGGACCTCAAACAGAAGCTTCCCAAGGCTTCCCCAGAA GGGATGAAGTATCACAAGCATGCATCCATCCTCGTCCACTGCAGAGATGGATCCCAGCAGACAGGGTTGT TCTGTGCCTTGTTCAATCTCTTGGAAAGTGCAGAAACAGAAGATGTGGTTGATGTTTTCCAAGTGGTAAA GTCTCTACGCAAAGCACGGCCTGGGGTGGTGTGCAGCTATGAGCAATACCAGTTCCTCTATGACATCATC GCCAGCATCTATCCCGCCCAGAATGGACAAGTCAAGAAAACAAACAGCCAAGACAAAATTGAATTTCATA ATGAAGTGGATGGAGGCAAGCAGGATGCTAACTGTGTCCGTCCAGATGGTCCTCTGAATAAAGCCCAGGA AGACAGCAGAGGGGTGGGAACCCCGGAGCCTACCAATAGTGCTGAGGAACCAGAACATGCTGCCAATGGT TCTGCGAGCCCAGCTCCAACCCAGAGTTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001268286
Insert Size	3393 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001268286.1, NP_001255215.1
RefSeq Size	5177 bp
RefSeq ORF	3393 bp
Locus ID	19264
Gene Summary	Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks four in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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