Grip1 (NM_001277293) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4931400F03Rik; eb; GRIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229379 representing NM_001277293 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGATGAGAGTCCCT ACACTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGA GGAATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCG GGAGGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTG ACCAGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGA GATCATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTC TCTGTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTG GTTTTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCG TCCTGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATT CGGCTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGC TGATAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGC CAAAACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTC ATAGACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCT CCATTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGA CCAGGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGTGAAA ATTCAGAGGAGCGACAGACAACATCCCTGGGATGCCTGGGCCAGCAACCAGTGCGGCGTTCATACCAACC ATCACCATAACACGTACCACCCAGACCATTGCAGAGTGCCAGCCCTGACTTTCCCGAAAGCGCTTCCTCC AAACAGCCCTCCGGCTATGGTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTG AACATGGGGACTTTACCTCGAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGA AAAAGAAAGACTTCAAAAGCTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCA CACTGAAACCACAGAGGTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGC GTGTTTGCCACAGAGACGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGA GATGTGGTGTGCTACAGATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTT CGAGGAAGCCAATCAACTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGAT GTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAAC TTGGAATAACCATCAGTTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAA AGGCAGTGTGGCACACAGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGG CTGGATAACTGTTCCATGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAA TCCGCAAAGATGAAGATAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAA GCGCTATGGGGGGCCCCTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCG AGCCTCACTAAAGGGGGATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCA ATAGCAGCAGCTTGAAGGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGT CACCCTGAAAATTAAGAAACAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGC CACTCGGGGGACCTAGGAGATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATG CGTACCCCTCCACGGTGCCCAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAG CTATGGGAGTCAAGGCTCAACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCA AAGCAAAGAACCAGCCTGTCCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTA ATGAAGACTGGGATCGATCCACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGA GGAAAACTTCTGGTCTCAAGCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTT GAGGCAACAATCATGTCGGGGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGG GGCGACAGGCCAGCTTCCAGGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACAC CCTGCCCTCAGACGTGGGCAGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATG TCCCCAACTCCGGTGGAGCTACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCA GTGTGGCAGATGGCCTGCTGGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGT TGGGGGCTTGAAGCCCTACGACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGC CTGGTGGTGCCTCTCATAGCTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCT CCCAGAAGTCGATAGAACAGCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCA ACCCAGCCACGGGATTCCTGGAGATACTGTATACTTTTGGCAAAGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277293
Insert Size	3342 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277293.1, NP_001264222.1
RefSeq Size	3959 bp
RefSeq ORF	3342 bp
Locus ID	74053
Gene Summary	This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (5) contains multiple differences at both the 5' and 3' ends, compared to variant 1. These differences include initiation of translation at an alternate start codon and use of an alternate 3' exon structure. The encoded isoform (5) is shorter and has distinct N- and C- termini, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.