Rgsl1 (NM_001243223) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930415K13Rik; Gm202; Rgsl2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229338 representing NM_001243223 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAACGCGGACATCATCTGTTCTACAAACCTTATCATTCTTTTGGAGGATGAAACATTTGCTGATT TTTTCAACACATTTCTTTCTCTCCCGGTTTTTGGTCAAACACCATTTTATAATGTTGAGAAATCACAATG GAGCTTGTGGCCAGAAGTACCTTATGACTTGATTGCCAAGTACAAAGGATTATTGACTTGGTTGGAAAAA TACCGGTTACCGTTCTTCTGCAAAACAAACTTGTGCTTCTACTACATCCTCAGCCAGGAGCTCGTCAGTT TCCTTAAGTCCCCGGAAGGAGGTGAAGAATTGCTGGATTTCTGGATCCTTGCTGAGAAGATTCTGAGCAT AGATGAGACAGACAAGTATTTGAAAGACTACTACCTCTCCCTCCTGCTCGTGCTCAAGGCCACCCACCTG CAGGAAGGCTCCAGAGTGGTGACTCTCTGCAACATGGACATCAAGTCCCTCTTGAACCTCTCCATCTGGC ATCCCAACCAGTCTACCACCAGAAGGGAGATCCTCAGCCACATGCAGAAAGTGGCTTTGTTCAAAATCCA GAGCTACTGGCTTCCCAACTTTTACACCCATGCCAAGATGACAATGGCCAACGAGGAAGCATGCCATGGT CTGATGCAGGAATATGAGACTCGCCTGTACAGTGTTTGCTGTGCACATGCAGGAGGGCTCCCTCTAAATA TGAGCATCAAGAAATCCCGCCACTCCCAAAAGAAGTACTCAAGCAAGAAGGCCAAGAAAAAGATATGGCA TTTGAATAATCCTATCTTATGGCCTCTGGAAACAGACGCTAAACCAGAAGTTCCCACTAAGCCCCAAGAA GAACTGAGTCTTCCAGAAAGGGTAGTTTTACAAAAGACTTCCCTGGGAGAAGATTCTTCAAAGGAGACAA TAATCCATTCCCTGTGCAAGGACTTGCAGAACGCCAAGAAACCCATTGTGAAGAAGAAATCCAAAATCCA ACTCCACATGGAGGGCTTCCTTGAGACAGCGTTCACTAATCACCTGAGATCTTCCACTCCCATTATTAAC CATGCTGCCCCGCTGGTTGTCAAGAAGTCTCTCAAGCGAAGCCTCTCCTTTGGGTACACTCATTGGGCCC TGTGTGCTGATGCCTGTGCAGGGAGTCCCTTCCGGTTTCACCTGAAGAAGATGAATCTGAAGGTGGAGGT CCAACTCCTGGACCTTTGGCAGGACCTACAGCATTTCATCAGTGTTCTCATGAATAATAGACAGAATGGG AATGCACTCTTTCGTCACATGCTTGGTAATCGAATCTGTGAGCTCTACCTGAACGAACAGATCGGGCCAC CCTTACCACTCAAACCTCAGACCCTTCAGGGCCTGAAGAAGCTTCTGCCATCTGGGGAGGTGAACCCCTG GATTCCCAAAGCCCAGAGGGAGATCTGCAAGGTGCTCAGCCCCTGGTATGATGAATTCCTGATGGAAGAG GACTACTGGTTTCTCATTTTCACAACTCAAACGCGCTTTGCCAAAGCCAGGTGGCATAAAAGAGAAACCA TCAGCAAAGAAGAACACATCCTCCTTTACAAAAGACTTCAGGAATCTCTGGTGCTAAGCCAGGCCTTGGC TAGCATGCAAGAAATAGATTCTATGCAGTGGCAAAAAGTGGCTACAGAGAACCTGCGGCAGGGCGGGTCT CTCCAAGTAGAGCTGACTACTCCAGTGTTCCTACAAGATATAAGTAAAATGTCCTTTAAGGATCTCTCCT TTAAGAATCCAAAGTTGGCCATAGAAAAAATGAGTGAAGACTACAAAATATACTGTGAAAAGGTACCTCC AACAGCTTTTACAGTGGAAATTATCAGGCAACCAAAATATTCATACTCACAAAGAAAAACATCCTTTGTC AAAAAATCTGTCGTAAGGAAGCCTTCTATGAGACCAAGGAACTTAACAGAAGTCCTCCTAAATAATGCAC ACCTCGAGTACTTCAAAGAGTTCCTCAAAGACCGGAAGTCTGAAAGCCCGTTGCAGTTCCTGATAGCCGT ACAGAAGATCATGATGGAGACCAATGAAAAGACTTATAAGACATCTTTGGAAAATATAACCAAGACTTAT CTCCACGGAAAGGTCCCTGCCGAGGAAATACTGCAGTGTGATGCTCCCTTTATCAAAGAAATTGCTAATG TGCGTCACATCACCACCACCACGTTGCTGATGCTCCAGGGTTATGTCATGAAATCTGTGGAAGAAAAATG GTTTAAGGAATACCAAGACTTGTTTCCACCACGATCTGTGGAGTTTGAGTTTGTTGAAGCACAGGCCGTC CCTAGGAAGCCCTCAAAGTCAACAACACACCTACACGACTCCCAGAAAAGGGGATGGATAAAAATGATCG CCTTCATCAAGAGTTTTTGCAACTACCGCAGATTCATAGCTGATGCCAACAATCGCCAAGAGCTGGCAGA TTTCATCTACCTGGAAATGTTCAACAACAAAGAGAATTTCTCTACATCACCCGCCACATCTGCACGTCAT ACCCCAGTAAACCTTAGTGCCCGGAACGCAGAACAGGAAAATGGAGACACGGTCCTTGTAAAACGCCGGA TATTTGGCCACAGAATTATCACGATCAACTTTGCCGTTAACGATATGTATTTATTTTCTGAAATGGAGAG ATTTAATGACCTGGTGAGTTCAGCTCACATGTTACAGATCAACAGAGCATACAATGAAAATGACATCATC CTGATGCGGTCTAAACTTAACATTATCGTGAAGCTTTACCTGGTTTCTGATCTTCCTCCAAAGCTGAAGG TGAACATCTCCGAGTCTCAGAAGGACATCATCATCTCTGCCATCACAGAAGGCCACTTAGACCGGACCAT CTTCCATGGGGCTATCATGTCCATCTTCCCTGTCATTATGTACTTCTGGAAAAGGTTTTGTAACTGGAAG GCGACACGCTCTTACGTAGAGTACCTTGGGAAGAAGTTTGAAGATGGAAGAAATCCTCCCAAGTCTGTAT ACAAGTACCCACCATGGAGTGGAGGTGAGCATACTGTCTTAAGGTTCAGCTTGCTCAGAGGTGTCGAATG GTTTCGACCTCAACACCCCCGGGATGCAGTGCCAAGTTCACTCCAACCATACCAAAAATATCCTACAACA AAAAAGAGAAGAAGTAATCAAGCCAAGGCCAAGGCAGATATGGATGGCCCTTTAACACTCTCCCAGTACT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243223
Insert Size	3222 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243223.1, NP_001230152.1
RefSeq Size	3640 bp
RefSeq ORF	3222 bp
Locus ID	240816

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