Ogdh (NM_001252287) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229282 representing NM_001252287 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACA ACCTAACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGATACGA GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTG ACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCA CTTACCCACCACCACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTG GAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCATGTTCATTAATGATTTGGAACAATGCCAGTGGA TCCGACAGAAGTTTGAGACCCCTGGAATCATGCAGTTCACCAATGAGGAGAAGCGGACCTTGCTGGCCAG GCTTGTACGATCCACCAGGTTTGAGGAGTTCCTACAGCGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTG GAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAATCATTGATATGTCAAGTGCAAATGGAGTGGACT ATGTGATCATGGGGATGCCACACAGAGGACGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGA GCAAATATTCTGTCAGTTTGACTCAAAGCTGGAGGCAGCTGATGAGGGTTCTGGGGACATGAAGTACCAC CTGGGCATGTATCACCGCAGGATCAACCGTGTGACCGACAGAAACATCACTTTGTCCTTGGTGGCTAACC CTTCCCATCTAGAGGCTGCTGACCCTGTCGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGA CACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCTTTTGCTGGCCAGGGCATCGTA TATGAGACCTTCCATCTCAGCGACTTGCCGTCCTACACAACCCATGGCACTGTTCATGTGGTTGTCAACA ACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCGCTCCTCTCCCTACCCCACTGATGTGGCCCGAGT GGTGAATGCCCCCATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCA GCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTAGTTGATCTGGTGTGTTATCGACGAAATGGCCACA ATGAGATGGACGAACCTATGTTTACACAGCCACTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTACT GCAGAAGTATGCAGAATTGCTAGTCTCCCAGGGTGTCGTCAATCAGCCTGAGTACGAGGAGGAAATCTCC AAGTATGATAAGATCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACT GGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCAC TGGCCTGGAGGAGGATGTCTTGTTCCACATTGGAAAGGTGGCCAGCTCTGTACCTGTGGAGAACTTTACT ATCCATGGAGGGCTGAGCCGGATCTTGAAGACCCGCAGAGAGCTTGTGACGAACCGGACTGTGGACTGGG CCCTGGCAGAGTACATGGCATTTGGCTCACTGCTGAAGGAAGGCATCCATGTGCGGCTGAGTGGCCAGGA TGTGGAGCGGGGCACCTTCAGCCATCGCCACCATGTGCTCCATGATCAGAATGTTGACAAAAGAACCTGC ATCCCCATGAACCACCTTTGGCCAAATCAGGCCCCTTACACTGTATGCAACAGCTCGCTGTCTGAGTACG GTGTCCTGGGCTTTGAGCTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTT TGGTGACTTCAACAACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTG CGGCAGAATGGCATTGTGCTCCTGCTGCCTCATGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCC GCCCAGAGCGGTTTCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTGACCTGCAGGAAGAAAACTT TGACATCAATCAGCTCTATGACTGCAACTGGATTGTTGTCAACTGTTCCACCCCTGGCAACTTCTTCCAT GTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGC GCCACCCTGAGGCAAGAACTAGCTTTGACGAGATGCTGCCAGGAACGCACTTCCAGCGTGTGATCCCAGA AAATGGACCTGCAGCTCAGGACCCACACAAAGTCAAGAGACTTCTCTTCTGCACTGGGAAGGTGTACTAT GACCTCACCCGAGAGCGCAAAGCCAGGAACATGGAGGAGGAGGTGGCTATTACAAGGATTGAGCAGCTAT CACCATTCCCCTTTGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCA GGAAGAGCACAAGAACCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGTACCACCATTGACCGTGCT AAGCCTGTCTGGTATGCTGGCCGAGACCCGGCAGCTGCTCCAGCCACTGGCAACAAGAAAACACACCTGA CAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCTGGACGCATTCAAGAAATTCTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252287
Insert Size	3072 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252287.1, NP_001239216.1
RefSeq Size	6706 bp
RefSeq ORF	3072 bp
Locus ID	18293
UniProt ID	Q60597
Gene Summary	2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and a region of the coding sequence, compared to variant 1. The resulting isoform (3) has a shorter and different internal segment, compared to isoform 1. Variants 3, 4 and 8 encode the same isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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