Dlgap3 (NM_001302081) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC058433; DAP-3; DAP3; Prpl8; Sapap3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229242 representing NM_001302081 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGTTACCATGGCGACCGAGGCAGCCATCCCCGCCCAGCCCGCTTTGCTGACCAACAGCATATGG ACGTGGGCCCAGCTGCCAGGGCCCCGTACCTGCTGGGCTCCAGGGAGGCCTTCTCCACCGAGCCCCGCTT CTGTGCTCCGAGAGCTGGCCTGGGACACCTTTCTCCAGAAGGACCCCTGAGCCTGAGTGAGGGTCCATCA TCAGTAGGCCCTGAGGGAGGCCCAGGGGGGGTGGGGGCTGGGGGAGGCAGCAGTACCTTCCCCAGGATGT ACCCCGGCCAGGGCCCCTTCGACACCTGTGAAGACTGTGTGGGCCACCCACAGGGCAAGGGTGCCACCCG ACTGCCTCCCACACTCCTGGACCAGTTTGAAAAGCAATTGCCAGTCCAACAAGATGGCTTCCACACACTC CCATACCAGCGAGGTCCAGCAGGGCCTGGTCCTGGACCTGGATCTGGCGCTGCCCCCGAGGCTCGCAGCG AGAGTCCTAGCCGCATCCGGCACCTGGTTCACTCAGTGCAGAAGCTCTTTGCCAAGTCCCACTCTCTGGA GGCACCCGGGAAGCGAGACTATAACGGGCCCAAGGCCGACGGAAGAGGTAGCTCTGGGGGAGACAGCTAC TCTGGTCCAGGCTCTGGAGGCACCCCCACCTCCCACCACCACCATCACCACCACCATCACCACCACCACC AGTCCCGGCATGGCAAACGGAGCAAGAGCAAGGACCGCAAGGGGGATGGGCGACACCAGACCAAGGCCAC AGGCTGGTGGAGCTCTGATGACAATTTGGACAGTGACAGTGGCTTTCTGGGTGGGAGACCTCCTGGAGAG CCTGGTGGCCCCTTCTGCCTGGATGCTCCAGATGGGTCCTACAGGGACTTGAGCTTCAAGGGGCGTTCGG GCGGGTCGGAAGGCCGCTGCCTTGCCTGCACTGGCATGTCCATGTCCCTGGATGGACAGTCAGTCAAGCG AAGTGCCTGGCATACCATGATGGTCAGCCAGGGCCGGGATGGATACCCAGGGGCCGGGCCAGGCAAGGGG CTCCTGGGTCCAGAGACCAAGGCCAAAGCCAGGACTTATCACTATTTGCAGGTGCCGCAAGATGACTGGG GGGGTTACCCCACGGGTGGCAAGGATGGGGAGATCCCCTGCCGCAGGATGCGGAGCGGCAGCTACATCAA AGCCATGGGGGATGAGGAGAGTGGAGACTCAGATGGTAGCCCCAAGACGTCTCCAAAAGCACTTGCCCGA CGCTTTGCCTCCCGCCGTTCCTCCAGCGTGGACACAGCCAGGATCAACTGCTGTGTCCCACCCCGGATCC ATCCCCGGAGTTCCATCCCTGGCTACAGCCGTTCCCTCACAACTGGACAGCTAAGCGAAGAGTTTAACCA GCAGCTGGAGGCCGTGTGCGGGTCCGTGTTTGGGGAGCTTGAGTCCCAGGCCGTGGACGCCCTGGACCTG CCCGGCTGTTTCCGCATGCGGAGCCACAGCTACCTCCGGGCCATCCAGGCCGGCTGCTCTCAAGACGACG ACTGCCTACCCCTCCTTGCTGCCCCTGCCTCTGTCTCAGGGAGGCCCGGCTCCTCCTTCAACTTCAGAAA GGCCCCGCCCCCCATCCCGCCGGGAAGCCAGGCCCCACCCCGCATCTCCATCACCGCCCAGAGCAGCACC GACTCTGCCCACGAGAGCTTCACCGCAGCAGAGGGCCCGGCCCGGCGCTGCAGCTCCGCGGACGGGCTGG ACGGCCCCACCATGGGCGCACGCACCCTCGAGTTGGCACCGGTGCCACCCCGAGCCAGTCCCAAGCCCCC CACACTCATCATCAAGACCATCCCTGGCCGGGAGGAGCTGCGGAGCTTGGCACGACAGCGGAAGTGGCGG CCGTCCATTGGGGTGCAGGTGGAGACAATCTCAGACTCGGACACCGAGAACAGGAGTCGAAGAGAATTCC ACTCCATTGGCGTTCAGGTGGAAGAGGACAAGAGGCGGGCGAGGTTCAAGCGCTCCAACAGCGTGACAGC CGGTGTGCAGGCAGACCTGGAGCTGGAGGGCCTGGCCGGCCTGGCCACTGTGGCCACAGAAGACAAGGCC CTGCAGTTCGGACGCTCCTTCCAGAGGCACGCCTCTGAGCCCCAGCCCGGCCCTCGGGCCCCCACCTACT CAGTCTTCCGCACGGTCCACACACAGGGCCAGTGGGCCTACCGCGAGGGCTACCCACTGCCGTATGAGCC GCCGGCCACCGATGGGTCGCCTGGCCCTACCCCTGTCCCAGCCCCCGGTCCCGGGTCCGGCCGCCGTGAT TCCTGGATGGAGCGGGGTTCACGTAGCCTCCCCGACTCAGGCCGCACGTCCCCCTGCCCACGGGATGGCG AGTGGTTCATCAAGATGCTGCGGGCAGAGGTGGAGAAACTGGAACACTGGTGCCAGCAGATGGAGCGTGA GGCGGAGGACTATGAGCTGCCCGAGGAGATCTTAGAGAAGATTCGCAGTGCCGTGGGCAGTACGCAACTT CTCCTGTCTCAGAAGGTCCAGCAGTTCTTCAGGCTGTGTCAGCAAAGCTTGGACCCCACTGCGTTCCCTG TGCCCACCTTCCAGGACCTGGCCGGTTTCTGGGACCTCCTGCAGCTCTCCATTGAGGATGTGACCCTCAA GTTCCTGGAACTACAACAACTCAAGGCCAACAGCTGGAAACTCCTGGAGCCGAAGGAGGAGAAGAAGGTC CCTCCGCCGATACCAAAGAAGCCCTCGCGGGGGCGGGGCGTGCCGGTGAAGGAGCGCTCCCTGGACTCGG TGGACCGGCAGCGGCAGGAAGCGCGCAAGCGGCTCCTGGCGGCCAAGCGCGCCGCCTCGTTCCGCCACAG TTCGGCTACCGAGAGCGCGGACAGCATCGAGATCTACATCCCCGAGGCCCAGACCAGGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302081
Insert Size	2934 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302081.1, NP_001289010.1
RefSeq Size	4223 bp
RefSeq ORF	2934 bp
Locus ID	242667
UniProt ID	Q6PFD5
Gene Summary	May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Both variants 1 and 2 encode the same protein.

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