Pde2a (NM_001243757) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CGS-PDE; cGSPDE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229171 representing NM_001243757 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGACAGCCTGCAGCCAGCCAGGACCCCCTTGCACAGAAGCCAGAACCCCCAGGGAGTAGAGACG ACCGACTGGAGGATGCTTTGCTGAGCCTAGGCGCCGTTATCGACATTGCTGGCCTGCGACAGGCTGCCAG GGATGCCCTCTCAGCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGCTAGATGGGGAGTCCAGA CTGGTGTGTGAGGACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAAGCTGTGATCTCTCAGA AGCGGCTGAGTTGCAATGGGCTGGGACCTTCGGACCTACTGGGAAAGCCCTTGGCCAGGCTGGTGGCTCC ACTGGCTCCTGACATGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAAGTGTGGCAGCTGTC ATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTGAGGAACAGAGCTTGCAGGTGGTAGAGAAGCACGCTC TGGTAGCCCTGCGGAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACGTCAGTGGA TGCCTCTGAAGATCAAAAGGATGAGAAGGGGTACACCGACCATGACCGAAAGATCCTGCAACTGTGTGGG GAACTCTTTGACTTGGATGCTACTTCTCTGCAGCTCAAAGTCCTTCAATATCTGCAGCAGGAGACACAGG CCACTCACTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTACAGCTTTCCTGCAAGGTCATTGGAGACAA AGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACCATGGGACGTCTGGGGCAGGTGGTGGAAGACAAGCAG TGTATCCAGTTGAAGGACCTAACCTCTGACGATGTGCAACAGCTGCAAAACATGTTGGGTTGTGAGCTGC AGGCTATGCTGTGTGTCCCTGTCATCAGCCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAA CAAGCTTGGAGGAGATTTCTTCACAGATGAGGATGAACACGTGATCCAACACTGCTTCCACTACACAGGC ACGGTGCTCACCAGCACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCC AAGTGGCAAAGAACCTCTTCACCCACCTGGATGATGTCTCTGTCCTGCTACAGGAGATCATCACGGAAGC CAGAAACCTCAGCAACGCAGAGATCTGCTCGGTGTTCCTGCTGGATCAGAACGAGCTGGTGGCCAAGGTG TTCGATGGTGGCGTTGTGGACGATGAGAGTTATGAGATCCGCATCCCGGCGGACCAAGGCATCGCGGGCC ACGTGGCGACTACGGGCCAGATCCTGAACATCCCAGATGCATATGCCCATCCGCTTTTCTATCGCGGCGT AGATGACAGCACTGGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAGGAGGTC ATTGGTGTGGCTGAGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAGTTTGATGAGGACCTGGCCA CAGCCTTCTCCATCTACTGTGGCATCAGCATCGCCCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCA ATACCGCAGCCACCTGGCCAATGAGATGATGATGTATCATATGAAGGTCTCTGATGATGAATACACCAAG CTTCTCCACGATGGCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTTACCTACACGCCTC GGTCTCTGCCTGAGGACGACACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAA CTACAAAATTGACTGCCCAACTCTGGCCCGATTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCACCC TACCACAACTGGATGCACGCCTTCTCTGTCTCTCATTTTTGCTACCTGCTCTACAAGAATCTGGAGCTCT CCAACTACCTCGAGGACATCGAGATCTTTGCATTGTTTATTTCCTGCATGTGTCATGACCTGGACCACAG AGGCACAAACAACTCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCCGCACTCTACAGCTCAGAGGGCTCT GTCATGGAGAGGCACCACTTTGCTCAAGCCATTGCTATCCTCAACACCCACGGCTGCAATATCTTTGACC ACTTCTCTCGGAAGGACTATCAGCGCATGCTGGACCTGATGAGGGACATCATCTTGGCTACAGACCTGGC ACACCACCTCCGCATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAGGCAA CACCACAGGCTTCTTCTGTGCCTCCTCATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGA CCACCAGAAAGATTGCAGAGCTGATCTACAAAGAGTTCTTCTCCCAGGGAGACTTGGAGAAGGCCATGGG CAACCGACCGATGGAGATGATGGACCGTGAGAAGGCCTACATCCCTGAGCTTCAGATCAGCTTTATGGAG CACATTGCCATGCCTATCTACAAGCTTTTACAAGACCTGTTCCCCAAGGCGGCAGAGCTGTATGAACGTG TGGCCTCCAACCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAA CTCGCTGGATTTCCTGGACGAGGAATACGAGGTCCCCGATTTGGACGGCACCAGAGCTCCTGTCAATGGC TGCTGCAGCCTCGAGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243757
Insert Size	2751 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243757.1, NP_001230686.1
RefSeq Size	4115 bp
RefSeq ORF	2751 bp
Locus ID	207728
Gene Summary	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and has multiple differences in the coding region, including the use of an alternate start codon, compared to variant 1. The encoded isoform, PDE2A1 (see PMID:19632989) is shorter and has a distinct N-terminus, compared to isoform 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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