Scube1 (NM_001271473) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	7330410C13Rik; A630023E24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229153 representing NM_001271473 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCGGCGGCCGTGCGCTGGCACTTGTACCTACTGCTCGCTCTGGGAGCGCGCGGGCGGCTGGTGG GGGGCAGCGGGCTGCCAGGAGCCGTGGACGTGGATGAATGCTCAGAGGGCACAGACGACTGCCACATAGA TGCCATCTGCCAGAACACACCCAAGTCTTACAAATGTCTCTGCAAGCCCGGCTATAAGGGGGAAGGAAGG CAGTGTGAAGACATTGACGAGTGTGAGAATGACTACTACAACGGGGGCTGTGTCCATGACTGCATCAACA TCCCTGGGAACTACAGGTGCACCTGCTTTGATGGCTTCATGCTGGCCCATGATGGACACAACTGCCTGGA TGTAGATGAGTGTCAAGACAACAACGGCGGCTGCCAGCAGATCTGCGTCAATGCCATGGGCAGCTATGAA TGCCAGTGCCACAGCGGCTTTTTTCTCAGTGACAACCAACACACCTGCATACACCGCTCCAATGAGACAT GTGCAGTGAACAACGGAGGCTGTGACCGGACGTGTAAGGACACTGCCACTGGCGTGCGGTGCAGCTGCCC GGTTGGATTCACGCTGCAGCCAGATGGAAAGACATGCAAAGACATCAACGAGTGCTTGATGAATAACGGA GGCTGCGACCACTTCTGCCGAAACACCGTGGGCAGCTTTGAATGCGGCTGCCAAAAGGGCCACAAGCTAC TGACGGACGAGCGGACATGCCAAGACATTGACGAGTGTTCCTTTGAGCGGACCTGTGACCACATCTGCAT CAACTCCCCCGGCAGTTTCCAGTGCCTGTGCCGCCGAGGCTACACACTCTACGGGACAACCCACTGCGGA GACGTGGATGAATGTAGCATGAACAACGGCAGCTGTGAGCAGGGCTGTGTCAACACCAAGGGCAGCTACG AGTGTGTGTGCCCACCAGGGAGGAGGCTGCACTGGAATCAGAAGGACTGTGTGGAGATGAACGGGTGCCT GTCTCGGTCCAAGGCCTCTGCCCAGGCCCAGCTGTCCTGTGGCAAGGTGGGTGGAGTGGAAAACTGCTTC CTGTCCTGCCTGGGCCACAGTCTCTTCATGCCAGATTCAGAAAGCAGCTACATCCTGAGCTGTGGTGTTC CAGGTCTCCAGGGCAAGACACTGCCAAAACGCAATGGCACTAGCTCCAGTACAGGGCCCGGCTGCTCAGA TGCCCCCACCACCCCTATCAGACAGAAGGCCCGCTTCAAGATCAGAGATGCCAAGTGCCATCTCCAGCCC CGCAGCCAGGAGCGAGCCAAGGATACCCTGAGGCATCCCCTTTTAGACAACTGCCACGTGACTTTTGTGA CCCTCAAGTGTGACTCCTCCAAGAAGAGGCGCCGAGGCCGCAAGTCCCCATCCAAGGAGGTGTCCCACAT CACAGCAGAGTTTGAGGTGGAGATGAAGGTGGATGAGGCCTCAGGCACCTGCGAAGCAGACTGCATGCGG AAGCGTGCCGAGCAGAGCCTGCAAGCCGCCATCAAGATTCTGCGCAAGTCCATTGGTCGGAACCAGTTCT ATGTCCAGGTCTTGGGCACTGAGTACGAGGTGGCCCAGCGGCCAGCCAAAGCACTGGAGGGAACCGGGAC TTGCGGCATAGGCCAAATACTACAGGACGGCAAGTGTGTACCGTGTGCTCCTGGCACCTACTTCAGTGGT GACCCAGGCCAGTGTATGCCATGCGTGTCGGGAACCTACCAGGACATGGAAGGCCAGCTCAGCTGCACGC CATGCCCCAGCAGCGAAGGACTGGGTCTGGCTGGTGCCCGAAACGTATCCGAATGTGGAGGCCAGTGCTC TCCAGGCTACTTCTCAGCGGATGGGTTCAAGCCTTGCCAGGCCTGCCCTGTGGGCACATACCAGCCCGAG CCTGGGCGAACCGGCTGCTTCCCCTGTGGAGGCGGGCTGCTCACCAAACACACGGGTACCGCCTCTTTCC AGGACTGCGAGGCCAAAGTGCATTGCTCTCCCGGACACCACTACAATACCACCACCCACCGGTGTATCCG CTGTCCCGTGGGCACCTATCAGCCCGAGTTTGGCCAGAACCATTGCATCTCCTGTCCGGGCAACACCAGC ACCGATTTTGATGGTTCCACCAACGTCACACACTGCAAGAACCAGCACTGCGGCGGCGAACTTGGTGACT ACACAGGCTACATCGAGTCCCCCAACTACCCTGGCGACTACCCAGCCAATGCAGAGTGTGTGTGGCACAT CGCACCTCCCCCAAAGCGCAGGATCCTAATCGTAGTCCCGGAGATCTTCCTACCCATCGAGGACGAGTGT GGTGATGTCCTGGTCATGAGAAAGAGTGCCTCGCCCACGTCAGTCACCACCTATGAGACGTGCCAGACCT ATGAGCGGCCCATCGCCTTTACCTCCCGTTCCCGCAAACTCTGGATCCAGTTCAAATCCAACGAAGCCAA CAGTGGCAAAGGGTTTCAAGTGCCCTATGTCACCTATGATGAGGACTACCAGCAGCTAATAGAAGACATC GTGCGTGATGGGCGCTTATATGCCTCGGAGAACCATCAGGAGATTTTGAAAGACAAGAAGCTGATCAAGG CCCTCTTTGACGTGCTGGCACACCCACAAAACTACTTCAAATACACAGCCCAGGAGTCCAAGGAGATGTT CCCCCGGTCCTTTATCAAACTTCTGCGTTCCAAAGTGTCTCGGTTCCTGCGGCCCTACAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271473
Insert Size	2724 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271473.1, NP_001258402.1
RefSeq Size	7599 bp
RefSeq ORF	2724 bp
Locus ID	64706
Gene Summary	Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks three alternate in-frame exons, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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