C77080 (NM_001285867) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Kiaa1522; mKIAA1522
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229139 representing NM_001285867 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAGAGCTCCACCGCGGAGGATGCGCTCTCCGTCCGCTCGGAGATGATTCAGCGCAGAGGCTCCA CCTTCAGACCCCATGACTCATTTCCCAAATCTGGGAAGTCAGGAAGGCGACGGAGGGAGCGAAGGAGCAC GGTGCTGGGGCTGCCTCAGCACGTGCAGAAGGAGCTCGGCCTGCGGAACAACCGTGAGGCACCGGGCACT CCACAGCCTCCTGGTTCACGGGACGCTGTCCGCATCCCCACGGTGGATGGTCGCCCCGGTCTGGCCTTAG GGACAGGGGTCCGAGTGTCCCTGCAGGCTCTGGAAGCAGAAACAGAGGCCGGCACAGATGCAGAGGCTGT CATCCAGCGCCACATCGACCGTGTTTACCACGATGACACGCTTGTTGGCCGATCCACGGGAGCCCGGCCA CCGCCACTGACGAGGCCAATGTCTCTCGCAGTGCCTGGACTGACAGGAGGAGCAGGATCTCCAGAGCCAC TGAGCCCAGCCATGTCAATCTCACCCCAGGCCACCTACTTGTCGAAGCTGATCCCGCACGCCGTGCTGCC GCCCACCGTGGATGTGGTGGCCCTGGGCCGCAGCAGCCTGCGCACTCTGAGCCGTTGCAGCCTGCTCTCT GCCAGCCCAGCTTCGGTTCGCTCCCTGGGCCGCTTCTCCTCGGCTTCCAGTCCACGGCCCCGCAGCCGCA ACGCTTCCTCGTCCAGTGACAACTGGAGCCACTCTCAGTCCTCCGAGACCATTGTGTCTGATGGTTCCAC TCTTTCCTCTAAGGGGGGCTCTGAGGGCCAGCCAGAAGGCTCTGTAGCTAGCAATAATGTGGCGCCCCCT CCTCCAGGCGGTAGTGGGCGGGGCTCCCCCAGCGGGGGTAGCACTGCGGAGACCTCAGACACGGCCAGCA TCCGAAGCAGTGGGCAGCTGTCTGGCAGGAGTGTGTCCCTGCGTAAGATGAAACGGCCTCCCCCGCCTCC CCGCCGGACCTACTCCCTCCATCAGCGCGGCTCCGCAGTGCCTGATGGGCCCTTAGGGTTGCCGCCCAAA CCTGAGCGAAAGCAGCAGCCACAGCTGCCTCGGCCGCCCACTGCGGGTGGCTCTTCAGGGGTGGGGGCAG TATCTTGTCCACCCAGCTCAGCAGGCACCTGGGGCTCTGGCTTGTCCCCAGGTGGCTCCAGGCGTCCCCC ACGTTCCCCAGAACGGACACTTTCACCTTCAAGTGGCTACTCGAGCCAGAGCGGTACCCCAACTCTCCCT CCCAAGGGTCTGGCAGTTGCCCCTGCTTCCCCAGGCAAGGCTCAGCCCCCCAAACCAGATCGAGTGACAT CCCTTCGATCTCCTGGGGCCTCTGTATCCTCTTCCCTTACATCTCTGTGTTCCTCTTCTTCAGACCCTAC TCCTTTAGACCGCTCTGGCCCACAAATGTCTACCCCCTTGAGTGACAGGTTCGTCATACCTCCTCATCCC AAGGTGCCTGCTCCTTTCTCTCCACCACCTTCCAAGTCCAAGAGCTCGAACCAAGCTGCTCCTGTTCTGG CTGCCCCTGCTGTGGCTCCTGGGCAGGTGTCCACCATCGACACCAGTCCTGCATCCCCTTCCATGCCCCA GACAACCTTGACTCCAGCCCAGGAGTCTCCTGTTGCCTCCAAAGATGAGTCACCCCCACCATCCCCACCC CCATCTTACCATCCACCCCCACCACCTACTAAGAAGCCGGAGGTGCTGGAGGAGGCCCCACCTCCTCCGG AAGCTGCTGTGGAGATCCTTCCAGATCCCAGCTGGCCGCCGCCACCACCACCTGCACCTGAGGAACAGGA CCTGTCGATGGCTGACTTCCCCCCTCCTGAGGAGGTCTTCTTCAATGCAGGCCCTGAGCTTGGCCCTCTG GAGTCCTGCAGCTCTGAGGCTGCCGTCCCCCCAGCTGCTAGCTTGTCCCAGACTCCTCCGCCAGCTCCAC CTCCTAGTTCTGGATCAGAACCTCTGGCCAGGCTCCCACAGAAGGACTCAGTGGGCAAGCACAGCGGGGC TCCCAGGGAGGATTCGGGCACGCCTCTGGTCACACCCTCGCTCCTGCAGATGGTCCGGCTTCGCTCTGTG GGTGCTTCCACAGGGATTCCGAACCCTTCTCCGGGTTCATCGGCCCCTCAGAAGCCTCTGCGAAGAGCCC TGTCTGGGCGCGCCAGCCCAGTGACTGCTCCCTCCTCTGGGCTCCATGCTGCCGTCCGACTCAAGGCCTC TAGCCTGGCTGCCAGTGAGAGTCCTGCGAGTGCTCTGCCCACTGGAATACCCGAGGCAGAGCCACGGTCG CCACAGTCTCCTGCCTCCAAGGCCAGCTTCATCTTCTCCAAGGGCACCAAAAAACTGCAGCTGGAGAGGC CCGTGTCCCCCGAGGCCCAGGCTGACCTCCAGCGGAATCTGGTGGCTGAACTTCGGAGCATTTCAGAGCA TCGGCCACCTCCCCAGGCCCAGAAGAAGCCTTCCAAGGCTCCCCCACCAGTGGCCCGCAAACCCTCAGTG GGAGTCCCTCCTCCGTCCCCCAGTCTTCCCAGGACGGAGTCTCTTACTGCTCCATCCACCAATGGGCTCC CTCACGCTGAGGACAGGACTAACGGGGAGCTGGCGGAGAATGGAGGTGTGCAGCTGGCTGCTACAGAGAA GATGGGCTCCCCTGGTTCAGATCCACAGAAGAAACTGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285867
Insert Size	2703 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285867.1, NP_001272796.1
RefSeq Size	4955 bp
RefSeq ORF	2703 bp
Locus ID	97130
UniProt ID	A2A7S8

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