Tyro3 (NM_001290800) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI323366; Brt; Dtk; Etk-2; etk2/tyro3; Rse; Sky; Tif; tk19-1; TK19-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229096 representing NM_001290800 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACAAACTGGAAAACACTCTGGGAAGATGGGCTGGGGAGAATGGTCTTTCGATAGGAGAATACT TGGCGATTAAAGGCCTGAAGCTCATGGGCGCCCCAGTGAAGATGACCGTGTCTCAGGGGCAGCCAGTGAA GCTCAACTGCAGCGTGGAGGGGATGGAGGACCCTGACATCCACTGGATGAAGGATGGCACCGTGGTCCAG AATGCAAGCCAGGTGTCCATCTCCATCAGCGAGCACAGCTGGATTGGCTTACTCAGCCTAAAGTCAGTGG AACGGTCTGATGCTGGCCTGTACTGGTGCCAGGTGAAGGATGGGGAGGAAACCAAGATTTCTCAGTCAGT ATGGCTCACTGTCGAAGGTGTGCCATTCTTCACAGTGGAACCAAAAGATCTGGCGGTGCCACCCAATGCC CCTTTTCAGCTGTCTTGTGAGGCTGTGGGTCCTCCAGAACCCGTAACCATTTACTGGTGGAGAGGACTCA CTAAGGTTGGGGGACCTGCTCCCTCTCCCTCTGTTTTAAATGTGACAGGAGTGACCCAGCGCACAGAGTT TTCTTGTGAAGCCCGCAACATAAAAGGCCTGGCCACTTCCCGACCAGCCATTGTTCGCCTTCAAGCACCG CCTGCAGCTCCTTTCAACACCACAGTAACAACGATCTCCAGCTACAACGCTAGCGTGGCCTGGGTGCCAG GTGCTGACGGCCTAGCTCTGCTGCATTCCTGTACTGTACAGGTGGCACACGCCCCAGGAGAATGGGAGGC CCTTGCTGTTGTGGTTCCTGTGCCACCTTTTACCTGCCTGCTTCGGAACTTGGCCCCTGCCACCAACTAC AGCCTTAGGGTGCGCTGTGCCAATGCCTTGGGCCCTTCTCCCTACGGCGACTGGGTGCCCTTTCAGACAA AGGGCCTAGCGCCAGCCAGAGCTCCTCAGAATTTCCATGCCATTCGTACCGACTCAGGCCTTATCCTGGA ATGGGAAGAAGTGATTCCTGAGGACCCTGGGGAAGGCCCCCTAGGACCTTATAAGCTGTCCTGGGTCCAA GAAAATGGAACCCAGGATGAGCTGATGGTGGAAGGGACCAGGGCCAATCTGACCGACTGGGATCCCCAGA AGGACCTGATTTTGCGTGTGTGTGCCTCCAATGCAATTGGTGATGGGCCCTGGAGTCAGCCACTGGTGGT GTCTTCTCATGACCATGCAGGGAGGCAGGGCCCTCCCCACAGCCGCACATCCTGGGTGCCTGTGGTCCTG GGCGTGCTCACCGCCCTGATCACAGCTGCTGCCTTGGCCCTCATCCTGCTTCGGAAGAGACGCAAGGAGA CGCGTTTCGGGCAAGCCTTTGACAGTGTCATGGCCCGAGGGGAGCCAGCTGTACACTTCCGGGCAGCCCG ATCTTTCAATCGAGAAAGGCCTGAACGCATTGAGGCCACATTGGATAGCCTGGGCATCAGCGATGAATTG AAGGAAAAGCTGGAGGATGTCCTCATTCCAGAGCAGCAGTTCACCCTCGGTCGGATGTTGGGCAAAGGAG AGTTTGGATCAGTGCGGGAAGCCCAGCTAAAGCAGGAAGATGGCTCCTTCGTGAAAGTGGCAGTGAAGAT GCTGAAAGCTGACATCATTGCCTCAAGCGACATAGAAGAGTTCCTCCGGGAAGCAGCTTGCATGAAGGAG TTTGACCATCCACACGTGGCCAAGCTTGTTGGGGTGAGCCTCCGGAGCAGGGCTAAAGGTCGTCTCCCCA TTCCCATGGTCATCCTGCCCTTCATGAAACATGGAGACTTGCACGCCTTTCTGCTCGCCTCCCGAATCGG GGAGAACCCTTTTAACCTGCCCCTCCAGACCCTGGTCCGGTTCATGGTGGACATTGCCTGTGGCATGGAG TACCTGAGCTCCCGGAACTTCATCCACCGAGACCTAGCAGCTCGGAATTGCATGCTGGCCGAGGACATGA CAGTGTGTGTGGCTGATTTTGGACTCTCTCGGAAAATCTATAGCGGGGACTATTATCGTCAGGGCTGTGC CTCCAAATTGCCCGTCAAGTGGCTGGCCCTGGAGAGCTTGGCTGACAACTTGTATACTGTACACAGTGAT GTGTGGGCCTTCGGGGTGACCATGTGGGAGATCATGACTCGTGGGCAGACGCCATATGCTGGCATTGAAA ATGCCGAGATTTACAACTACCTCATCGGCGGGAACCGCCTGAAGCAGCCTCCGGAGTGCATGGAGGAAGT GTATGATCTCATGTACCAGTGCTGGAGCGCCGACCCCAAGCAGCGCCCAAGCTTCACGTGTCTGCGAATG GAACTGGAGAACATTCTGGGCCACCTGTCTGTGCTGTCCACCAGCCAGGACCCCTTGTACATCAACATTG AGAGAGCTGAGCAGCCTACTGAGAGTGGCAGCCCTGAGGTCCACTGTGGAGAGCGATCCAGCAGCGAGGC AGGGGACGGCAGTGGCGTGGGGGCAGTAGGTGGCATCCCCAGTGACTCTCGGTACATCTTCAGCCCCGGA GGGCTATCCGAGTCACCAGGGCAGCTGGAGCAGCAGCCAGAAAGCCCCCTCAATGAGAACCAGAGGCTGT TGTTGCTGCAGCAAGGGCTACTGCCTCACAGTAGCTGTTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290800
Insert Size	2631 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290800.1, NP_001277729.1
RefSeq Size	4469 bp
RefSeq ORF	2631 bp
Locus ID	22174
UniProt ID	P55144
Gene Summary	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains alternative 5' exon structure, and it thus differs in its 5' UTR and initiates translation from an alternative start codon, compared to variant 1. The encoded isoform (B) has a distinct N-terminus and is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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