Poln (NM_001289804) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	POL4P
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229006 representing NM_001289804 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGGAAAATTATGAGGCATGTGTAGGTTTTGATGTCTGTGAGATACCCCTCTCTGCTGTTGCTC AGAAGATTATGTCTGCTATGCGTTCAGGTGATTTCATGGATTCTAGGAATGAGGGAGAGAGTACAAACAC TTCAAAAGTGGCAAAGAAATCCAGTGTTCATTACTCAGTATTAGCTGAACATGAGGAGACTCAATCACTA GGAACAAAGAACCCCGAGTCATTAATAACCCAGACACCAAGAGGATCCATTGAGCTCTGTCCTCAGCCCT CCATTACTAAGCTCACATGTCAGCTCTCTGCTGGTCAAGTACAGAACAGCATCAGCTCATTGGGTCTTTC CAGTTATTTAATTCCACAGTGTGATCAGGAAGCTTCAGTTTTACCGAACATGGAGCATAAGAGACAGCAT TTCCTAAAGGAAAATATAGGCAAAGAAGATAAGGACAACTCGAGTCTTAAAAGAAAATATATTACATGTA GTAAATCCTCAGAAAAAGCAAGTAAACACACAGCTCTGGAAAAAGATACTGATGGGACTGAATCCTGGCC AAACTCCAGGGACACAAGAGCTTTGGGGGAAAGGCTGTGTGATGTTAGATATTTGGGTGATTTGGCCAAA GCCCAGCTGATGGATGCTCTCAAGCAGGCAGCAGCCCTGGTGGTGACATTGATGTACAAGGATGGTTCCA CACAGCTGAGCGCCAAGGAGGCTTTAACTTGTACTGTTAAAGGAATTGTAGTGTTACTAAAAAGCCATGT AGGCAACAGCACTCTGACTCTCCCAGCCCATGGTGGTGCTCTGGAAAAGGACTTCATATCCGAAGATCAC TGTGTCTACATACATACGGAGCATTCCCCTTTCTGGGACCCAAAGCAAGAAGCACATAGTCTATTTGTCA GGAACATACTGTTTTGGACACTGAGGTGTAAATGTCCCGTTGTTTGTTTTAATGCCAAGGACTTTGTGAG AACAGTGCTGCAGCTCTATGGTGAAGATGGCAGTTGGAAGCATGTTGCTGATTTTGTAGGACTAGATCCC AGAGTTGCTGCATGGCTCATAGACCCCAGTGACACTGCACCTTCATTTGAAGATTTAGTAGCAAAACACC TAGAAAAGTCCATCACAGTCAAACCAAGCAGCACATTCAGAGAAGCCTCAAGAAATACTTTGAGTCAGAA TGTATTTATGAATCTGAAGATACTCTACGACCTTACAATGGATCTTTGTTCTAAGTTGAAGGCTTATGGC TTATGGCAGCTATTTTGCACTTTGGAGCTTCCATTGATACCAATTTTGGCAGTGATGGAAAACCATAAGA TTCCAGTTGACAAAGAAGAAATGGAGAGGACATCGGCACTCCTCGGGGCTCGCCTCAAGGAATTGGAGCA AGAAGCCCATTTTGTTGCAGGAGAACAGTTTCTTATAATGAGTAATAATCAACTTCGTGAGATCCTCTTT GGCAAGTTGAAGCTGCACTTGTTGAGTCAGAGGAAACATCTTCCCAGAACTGGGCTGCAGAACCAGCTGT CCACATCAGAAGCCATGTTAAATTCTCTACAAGACCTTCACCCATTACCCAAGTTAATTTTGGAATATAG ACAGGTTCACAAGATCAAGTCAACTTTTATAGATGGATTACTAGCTTATATGAAAAAGGGCTCCATCTCT TCTACATGGAATCAGACTGGAACTGTGACAGGGAGACTTTCAGCCAAGCATCCAAATATCCAGGGTATCT CCAAGCACCCAATTAAGATTTCTAAGCCTTGGAATTTTAAAGGTAAAGAAGAGGAGACTGTCACCATCTC ACCAAGAACCCTGTTTGTGTCCTCTGAAGGCCACACCTTCCTGGCAGCAGACTTTTCACAGATTGAATTG CGCATTCTTGCACATTTATCTGGGGATCCAGAGCTTTTGAAGTTATTCCAGGAATCTGAAAGAGATGATG TATTTTCTACCCTGACTTCACAGTGGAAGGACATTCCCATAGAGCGTGTGACACACATGGACAGAGAACA AACCAAGAAAGTGGTGTACTCCGTGGTCTATGGAGCTGGCTATGTGACATCCATCTTGGGTAGAAGGAGG CCTCTCCCGAGGATCTGTGCACAGGACCAGCAGCTTCGGGCACAAGCAGAGCGACAGGCAGTAAACTTTG TGGTACAAGGCTCAGCCGCAGACCTCTGTAAGCTAGCCATGATTCGCATCTCTACTGCAGTAGCCACATC CCCGACCTTGACAGCCAGGTTAGTTGCCCAAATTCATGATGAGCTGCTGTTTGAAGTAGAAGACACTCAG GTCCCTGAGTTTGCAGCTCTTGTCAGAAGGATCATGGAGTCCTTGCAACAGGTACAGACCCTGGAACTGC AGCTGCAGGTGCCCCTAAAAGTGAACTTGAGTGTTGGCCGCTCATGGGGACACCTGACCCCACTGCAGGA GATCCTGGGCTCAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289804
Insert Size	2469 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289804.1, NP_001276733.1
RefSeq Size	2911 bp
RefSeq ORF	2469 bp
Locus ID	272158
UniProt ID	Q7TQ07
Gene Summary	DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template. Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ). Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity. Error-prone DNA polymerase that preferentially misincorporates dT regardless of template sequence. May play a role in TLS during interstrand cross-link (ICL) repair. May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross-links. May have a role in cellular tolerance to DNA cross-linking agents. Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 3, which lacks an internal segment and is shorter than isoform 1.

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