Nell2 (NM_001289653) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A330108N19Rik; mel91; R75516
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228996 representing NM_001289653 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGCCATGGAATCCCGGGTGTTACTGAGAACGTTCTGCGTGATCCTCGGGCTTGGAGCGGTTTGGG GGCTTGGTGTGGACCCCTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCTACAGATGG AGTGCGCCAAGTCCCGGGACTGCATAATGGGACGAAAGCCTTCCTCTTCCAAGAGTCCCCCAGAAGCATA AAGGCATCCACTGCTACAGCTGAGCGGTTTTTCCAGAAGCTGAGAAATAAACACGAGTTCACAATCTTGG TGACCCTGAAACAGATCCACTTAAATTCGGGAGTTATCCTCTCCATCCACCACTTGGATCACAGGTACCT GGAACTGGAAAGCAGTGGCCATCGGAATGAGATCAGACTCCACTACCGCTCTGGCACTCACCGTCCCCAC ACGGAAGTGTTTCCTTATATTTTGGCTGATGCCAAGTGGCACAAGCTCTCCTTAGCCTTCAGTGCCTCTC ACTTAATTTTACACATCGACTGCAATAAGATCTATGAACGAGTGGTGGAAATGCCTTCAACAGACTTGCC TCTGGGCACCACATTTTGGTTGGGACAGAGAAATAATGCACATGGCTATTTTAAGGGAATAATGCAGGAT GTGCACGTTCTTGTCATGCCTCAGGGCTTCATTGCTCAGTGCCCGGACCTTAATCGAACCTGTCCAACAT GCAACGACTTCCATGGGCTTGTGCAGAAAATCATGGAGCTGCAGGACATTTTATCAAAGACGTCAGCCAA GTTGTCCAGAGCTGAACAAAGAATGAACAGGCTGGATCAGTGCTACTGTGAGCGGACATGCACTGTGAAG GGAACCACCTACCGAGAGTCTGAGTCCTGGACAGACGGCTGTAAGAACTGCACATGCTTGAACGGGACCA TCCAGTGCGAGACTCTGGTCTGCCCTGCTCCTGACTGCCCTCCTAAATCGGCCCCTGCGTATGTGGATGG CAAGTGCTGTAAGGAGTGCAAATCAACCTGCCAGTTCCAGGGACGGAGCTACTTTGAGGGAGAAAGGAAC ACGGTATACTCATCTTCTGGAATGTGTGTCTTATATGAATGCAAGGATCAGACCATGAAGCTTGTTGAGA ACATTGGCTGCCCACCCTTAGATTGTCCCGAGTCTCATCAGATTGCCTTGTCTCACAGCTGCTGCAAGGT TTGTAAAGGTTATGACTTCTGTTCTGAGAAGCATACCTGCATGGAGAACTCGGTCTGCAGGAACCTGAAC GACAGGGCTGTGTGCAGCTGCAGGGATGGTTTTCGGGCTCTCCGAGAGGACAACGCCTACTGTGAAGACA TTGACGAGTGTGCAGAAGGGCGCCATTACTGCCGTGAGAACACCATGTGTGTGAATACACCTGGTTCTTT CATGTGTATCTGCAAAACTGGGTACATCAGGATCGACGATTACTCATGTACAGAACATGATGAGTGTCTC ACAAACCAGCACAATTGTGATGAAAACGCTTTGTGCTTTAACACTGTTGGAGGACACAACTGTGTCTGCA AGCCTGGCTACACCGGGAATGGAACCACGTGCAAAGCTTTCTGCAAAGATGGCTGTAGAAACGGAGGAGC GTGCATTGCTGCCAATGTGTGTGCCTGCCCACAAGGCTTCACGGGACCCAGCTGTGAGACAGACATTGAC GAGTGCTCTGAGGGCTTTGTTCAGTGTGACAGCCGTGCCAACTGCATCAACCTGCCTGGGTGGTATCACT GTGAGTGCAGAGACGGCTACCATGACAATGGGATGTTTGCGCCAGGCGGAGAATCCTGTGAAGATATTGA CGAATGCGGGACTGGGAGGCACAGCTGCACCAACGACACCATTTGCTTCAACTTGGACGGGGGATACGAT TGCCGGTGTCCCCATGGGAAGAACTGCACTGGGGACTGCGTGCACGAGGGGAAAGTGAAGCACACCGGCC AGATCTGGGTGCTGGAAAACGACAGGTGCTCCGTGTGTTCCTGCCAGACTGGGTTTGTCATGTGTCGACG GATGGTCTGCGACTGCGAAAACCCCACAGTTGACCTTTCCTGCTGCCCTGAGTGTGACCCAAGGCTGAGC AGTCAGTGCCTGCATCAAAACGGGGAAACCGTGTACAACAGCGGCGACACCTGGGTCCAGGATTGCCGTC AGTGCCGCTGCTTGCAAGGAGAAGTTGACTGTTGGCCCCTGGCTTGCCCAGAGGTAGAATGTGAATTTAG CGTCCTTCCTGAGAACGAGTGCTGCCCACGCTGTGTCACCGATCCTTGTCAGGCCGACACCATCCGCAAT GACATCACCAAAACCTGCCTGGACGAGATGAACGTGGTTCGCTTCACCGGGTCTTCCTGGATCAAGCACG GCACGGAGTGTACCCTCTGCCAGTGCAAGAATGGCCATTTGTGCTGCTCAGTGGATCCACAGTGCCTTCA GGAGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289653
Insert Size	2460 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289653.1, NP_001276582.1
RefSeq Size	3168 bp
RefSeq ORF	2460 bp
Locus ID	54003
UniProt ID	Q61220
Gene Summary	Required for neuron survival through the modulation of MAPK pathways (By similarity). Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity).[UniProtKB/Swiss-Prot Function]

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