Cdh16 (NM_001252627) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228961 representing NM_001252627 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTCTGCCCGGCCGTGGCTACTTTACCTCTCTGTTATTCAGCTACCACTACCCCTGGGGAGAGATG AAGGCCACATTGTCCTATCAGGAGACTCAAACACGGCAGATCAAAACACCTTTGCTGTGGACACAGACTC TGGCTTTCTAGTGGCGACAAGGACCCTGGACCGGGAAGAGAAAGCAGAATACCAACTACAGGTCACCTTG GAGTCTGAGGATGGACGTATCTTGTGGGGTCCACAGCTTGTGACTGTGCATGTGAAAGATGAGAATGACC AGGTACCCCAATTCTCCCAGGCCATCTACAGAGCTCAGCTGAGCCAGGGCACCAGGCCTGGGGTCCCCTT CCTCTTCCTTGAGGCTTCTGATGGGGATGCACCAGGCACAGCTAACTCCGACCTTCGCTTCCACATTCTG AGCCAGTCCCCACCTCAGCCTTTACCAGACATGTTCCAGCTGGACCCTCACCTAGGGGCTCTGGCTCTTA GTCCCAGTGGAAGCACCAGCCTAGACCATGCCCTTGAAGAGACTTACCAGCTATTGGTACAGGTCAAGGA CATGGGTGACCAGCCTTCAGGCCACCAGGCTATTGCAACTGTAGAGATCTCCATAGTAGAGAACAGCTGG GCACCCCTAGAGCCTGTTCACCTGGCAGAGAATCTCAAAGTTGTGTACCCACACAGCATTGCCCAGGTGC ACTGGAGTGGAGGAGACGTGCACTACCAGCTGGAGAGCCAGCCTCCAGGACCCTTCGATGTGGATACAGA GGGGATGCTCCATGTTACCATGGAGCTGGACCGGGAGGCCCAGGCTGAGTACCAGCTCCAAGTCCGAGCT CAGAATTCCCATGGTGAGGACTACGCAGAACCCCTGGAGTTGCAGGTGGTGGTGATGGATGAAAACGACA ATGCACCTGTCTGCTCCCCACATGACCCAACAGTCAACATCCCTGAGCTCAGCCCCCCAGGAACTGAAAT AGCCAGGCTCTCAGCAGAGGATTTGGATGCCCCTGGGTCACCCAATTCCCACATTGTATATCAGTTGTTG AGCCCTGAGCCTGAGGAGGGGGCTGAAAACAAAGCCTTCGAGTTAGATCCGACCTCAGGCAGTGTAACAC TGGGAACTGCCCCACTCCATGCTGGCCAGAGTATCCTGCTTCAGGTGCTGGCTGTTGACCTAGCAGGATC AGAGAGTGGCCTCAGCAGCACATGTGAGGTGACAGTCATGGTGACAGACGTCAACAACCATGCCCCTGAG TTCATCAATTCCCAGATTGGGCCTGTAACTCTTCCTGAGGATGTAAAACCTGGGGCTCTGGTGGCAACAC TCATGGCCACTGATGCTGACCTTGAACCTGCCTTCCGCCTTATGGACTTTGCCATTGAAGAAGGAGACCC AGAAGGGATCTTTGACCTGTCCTGGGAGCCAGACTCCGACCATGTCCAGCTCAGACTCCGGAAGAACCTC AGCTATGAGGCAGCTCCTGATCACAAGGTGGTGGTGGTCGTGAGTAACATAGAAGAACTGGTGGGCCCAG GCCCAGGCCCTGCAGCCACAGCCACAGTGACTATACTAGTGGAGAGGGTGGTTGCTCCCCTCAAGTTGGA CCAGGAGAGCTATGAGACCAGCATCCCAGTCAGCACCCCAGCTGGCTCCCTCCTGCTGACCATCCAGCCC TCAGACCCCATGAGCAGAACCCTCAGGTTCTCCCTGGTCAATGACTCAGAGGGCTGGCTCTGTATCAAGG AGGTGTCTGGGGAGGTACACACAGCCCAGTCCCTGCAGGGTGCCCAGCCTGGAGACACATACACAGTGCT TGTGGAGGCCCAAGACACAGATAAGCCAGGACTGAGCACTTCTGCCACTGTTGTGATCCACTTCCTGAAG GCCTCTCCTGTCCCAGCATTGACTCTGTCTGCTGGGCCCAGCCGACACCTCTGTACACCCCGCCAAGACT ACGGTGTAGTTGTGAGTGGGGTCAGTGAGGACCCTGACCTAGCCAACAGGAATGGTCCCTACAGCTTTGC TCTCGGTCCCAATCCCACTGTGCAGCGGGATTGGCGCCTCCAGCCTCTCAACGATTCCCACGCCTACCTC ACCTTGGCATTGCATTGGGTAGAGCCTGGTGAATACATGGTACCTGTGGTTGTCCACCATGATACCCATA TGTGGCAACTCCAGGTCAAAGTGATTGTGTGTCGCTGCAACGTGGAAGGCCAATGTATGCGCAAGGTGGG TCGCATGAAGGGAATGCCCACGAAACTGTCAGCGGTGGGTGTCCTCTTGGGCACCCTGGCAGCGATAGGC TTCATTCTCATTCTTGTGTTCACGCACCTGGCCCTGGCAAGGAAGGACCTGGATCAGCCAGCAGACAGCG TGCCTCTGAAGGCAGCGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252627
Insert Size	2403 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252627.1, NP_001239556.1
RefSeq Size	2935 bp
RefSeq ORF	2403 bp
Locus ID	12556
Gene Summary	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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