Zer1 (NM_001290503) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C230075L19Rik; Zyg11bl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228890 representing NM_001290503 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTGACACGCCCGAGTCCCTGATGGCCCTCTGTACTGACTTCTGTCTCCGGAATCTTGATGGCA CCCTGGGCTACCTCCTGGACAAAGAGACCCTGCGGCTGCATCCAGACATCTTCTTGCCCAGTGAAATCTG TGACCAGCTGGTCAATGAGTATGTGGAGCTGGTCAGTGCTGCCTGCACTTTTGAGCCACACGAGACTTTC TTCAGCCTCTTCTCAGACCCCCGCAGTACTCGGCTCACTCGCATCCACCTTCGTGAGGACCTGGTGCAGG ACCAGGACCTGGAAGCCATTCGCAAACAGGACCTGGTGGAGCTCTACCTGACCAACTGCGAGAAGCTGTC CGCCAAGAGCCTGCAGACACTGCGGAGCTTCCGACACAGTCTGGTGTCCTTGAGTCTCTCCGGCTGTGCC AACATCTTCTATGAGGAGGACAACCCAGGCGGCTGTGAGGATGAGTGCCTGGTCAACCCCACCTGCCAGG TGCTGGTCAAGGACTTCACCTTCGAGGGCTTCAGCCGCCTGCGCTTCCTCAACCTGGGCCGCATGATTGA CGGTATCCCCGTAGAGTCACTGCTTCGGCCACTCAACTCCCTGGCCGCCTTGGACCTCTCAGGCATCCAG ACAAGTGATGCCACTTTCCTAACACAGTGGAAGGACAGTCTGATGTCCCTTGTGCTCTACAACATGGACC TTTCAGATGACCACATCCGTGTCATTGTCCAGCTGCACAAGCTGCGCCACCTGGACATCTCCCGAGACCG CCTCTCCAGCTACTACAAGTTCAAGCTGACTCGGAAGGTGCTCAGCCTCCTAGTGCAGAAGCTGGGGAAC CTAATGTCACTGGACATCTCTGGTCACATGATCCTAGAGAACTGCAGCATCTCCAAGACAGATGAGGAGG CAGGGCAGACCAGCACCGAGCCATCCAAGAGCAGCATCATGCCCTTCCGGGCTCTGAAGAGGCCACTGCA GTTCCTTGGGCTCTTTGAGACCTCATTGTGTCGTCTTACGCACATTCCAGCCTACAAAGTGAGTGGTGAC AAAAATGAAGAGCAGGTGCTGAACGCCATCGAGGCCTACACAGAGCACCGGCCCGAGATCACATCCAGGG CCATCAACCTGCTGTTTGACATTGCACGCATTGAACGCTGCAACCAGCTTCTGCGGGCCCTGAAGCTGGT CATCACAGCCCTCAAGTGTCACAAGTATGACAAAAACATTCAAGTGACCGGCAGTGCTGCCCTCTTCTAC CTGACCAACTCCGAGTACCGCTCGGAGCAGAGCGTCAAGTTGCGGCGGCAGGTCATCCAGGTGGTGCTGA ATGGCATGGAGTCCTACCAGGAGGTGACGGTGCAGCGGAACTGCTGTCTGACCCTCTGCAACTTCAGCAT CCCCGAGGAACTGGAGTTCCAGTACCGCCGGGTGAACGAGCTGCTGCTGGGCATCCTCAGCCCCACGCGT CAGGACGAGTCAATCCAGCGGATCGCTGTGCACTTGTGCAATGCCCTGGTCTGCCAGGTGGACAATGACC ATAAGGAGGCTGTAGGCAAGATGGGCTTCGTCGTGACCATGTTGAAGCTGATCCAGAAGAAACTGCTGGA CAAGACGTGTGACCAGGTCATGGAGTTCTCCTGGAGTGCTCTGTGGAACATCACAGATGAGACACCTGAC AACTGCGAGATGTTCCTCAACTTCAATGGCATGAAGCTCTTTCTCGACTGCCTGAAGGAGTTCCCAGAGA AGCAGGAGCTACACCGTAACATGCTAGGGCTCTTGGGGAATGTGGCAGAGGTAAAGGAGCTGCGACCTCA GTTGATGACCTCCCAGTTCATCAGCGTCTTCAGCAACCTGCTGGAGAGCAAGGCCGACGGCATTGAGGTT TCTTACAACGCCTGTGGTGTCCTCTCTCACATCATGTTTGATGGGCCTGAGGCCTGGGGTGTCTGTGAAC CTCAGAGGGCAGAGGTGGAGGATCGTATGTGGGCGGCCATCCAGAGCTGGGATATCAACTCCCGAAGGAA TATCAACTACAGGTCCTTTGAGCCAATCCTCCGCCTCCTTCCCCAGGGTATTTCTCCAGTCAGCCAGCAC TGGGCCACCTGGGCCCTGTACAACCTCGTGTCTGTCTACCCTGACAAGTACTGCCCCCTGCTGATTAAAG AAGGTGGGATGCCCCTGCTGAGAGACTTGATCAAGATGGCAACTGCCCGGCAGGAAACCAAGGAGATGGC CCGCAAGGTGATTGAGCACTGCAGTAACTTTAGAGAGGAGAACATGGACACGTCCAGATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290503
Insert Size	2301 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290503.1, NP_001277432.1
RefSeq Size	4241 bp
RefSeq ORF	2301 bp
Locus ID	227693
UniProt ID	Q80ZJ6
Gene Summary	Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZER1-CUL2-Elongin BC.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

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