Ampd3 (NM_001276301) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228889 representing NM_001276301 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGCCAGTTCCCCAAGTTGAACATGTCCGATTTGGACGAACATGTCCGGCTGTTGGCGGAGAAGG TGTTTGCTAAGGTGCTCCGAGAAGAGGACAGCAAAGATGTCATGTCCCTGTTTACTGTCCCCGAGGACTG TCCCATCGGACAGAAGGAAGCCAAGGAGAGGGAGCTACAGAAGGAGCTTGCAGAGCAGAAGTCTGTGGAG ACAGCAAAAAGAAAGAAGAGTTTCAAGATGATCCGGTCGCAGTCTTTGTCCCTGCAGATGCCAACACAAC AAGACTGGAAAGGTCCTCCAACTGCCAGTCCTGCCATGTCTCCTGCAACCCCTCTGGTTCCCGGAGCTAC TTCTAAGCCTGGGCCAGCACCCTATGCTATGCCTGAGTATCAGCGGGTCACCATCAGTGGAGATTACTGT GCAGGGATCACTGTGGAAGACTATGAGCAGGCTGCCAAGAGCCTGGCCAAGGCCCTGATGATCCGGGAGA AGTATGCACGGCTTGCCTACCACCGCTTCCCACGGACCACAGCCCAGTACCTGGCTCATCAGGGGGAAAG TGTGCCTTTGGAAGAGGGTCTCCCAGACTTCCACCCTCCCCCACTGCCCCAGGAAGACCCTTATTGCCTG GATGATGCACCCCCCAACCTGGGCTACCTGGTTCGCATGCACGGAGGTGTTCTCTTTGTGTATGACAATC AGACGATGTTGGAACGCCAGGAGCCCCACAGCCTGCCCTACCCTGACCTGGAGACCTACATTGTGGACAT GAGCCATATTCTGGCACTCATCACTGATGGCCCCACGAAAACCTATTGTCACCGGCGACTGAACTTTCTG GAATCCAAGTTTAGCCTTCATGAGATGTTAAATGAAATGTCAGAGTTCAAGGAATTGAAGAGTAACCCCC ACAGAGACTTTTATAATGTGAGAAAGGTAGACACACACATTCATGCAGCGGCCTGCATGAATCAGAAGCA CCTGCTGCGTTTCATCAAGCACACGTACCAGACAGAGCCCGACAGAACTGTGGCGGAGAAGCTAGGTCGG AAGATCACCCTGCGGCAGGTGTTTGACAGCCTGCACATGGACCCCTACGACCTCACGGTGGACTCGCTGG ATGTTCATGCAGGCCGCCAGACATTTCACCGCTTCGACAAGTTCAACTCCAAGTACAACCCCGTGGGGGC CAGCGAGCTCCGGGACCTGTATCTGAAAACTGAGAACTACCTCGGGGGAGAATACTTTGCTCGCATGGTC AAGGAAGTGGCCCGGGAGCTAGAGGACAGCAAGTACCAATACTCAGAACCCCGGCTCTCCATCTATGGTC GCAGTCCCAAGGAGTGGTCTAGCCTGGCCCGCTGGTTCATCCAGCACAAGGTCTACTCACCCAACATGCG CTGGATCATCCAGGTGCCCCGCATCTATGATATATTTAGGTCAAAGAAACTACTGCCAAACTTTGGGAAG ATGCTGGAGAACATCTTCCTGCCCCTGTTCAAAGCTACCATCAACCCCCAAGATCATCGGGAGCTTCACC TCTTCCTTAAATACGTGACTGGGTTTGACAGTGTGGATGACGAATCCAAGCACAGTGACCACATGTTCTC AGACAAGAGTCCCAGCCCAGACCTCTGGACCAGCGAGCAGAACCCGCCCTATAGTTACTACCTGTACTAC ATGTATGCCAACATCATGGTGCTGAACAACCTCCGCAGGGAACGAGGCCTGAGCACGTTCCTCTTCCGGC CTCACTGTGGGGAGGCCGGCTCCATCACTCACCTAGTGTCTGCCTTCCTGACGGCAGACAACATTTCCCA CGGGCTGCTGCTTAAGAAGAGCCCTGTTTTGCAGTACCTCTACTACCTTGCTCAGATCCCTATTGCCATG TCTCCTCTCAGCAACAACAGCCTGTTTCTGGAATATTCCAAGAACCCCCTCCGGGAATTCCTGCACAAGG GACTACATGTCTCTCTCTCTACCGATGACCCCATGCAGTTTCACTACACAAAGGAAGCGCTCATGGAGGA GTACGCCATTGCGGCACAAGTGTGGAAGCTGAGTACCTGCGACCTGTGTGAGATCGCCAGGAACAGTGTG CTGCAGAGTGGCCTCTCACATCAGGAGAAGCAGAAGTTTCTGGGTCAGAATTATTACAAGGAAGGACCAG AGGGCAATGACATTCGAAAGACAAATGTTGCACAGATCCGGATGGCATTCCGCTACGAGACCCTGTGTAA TGAGCTCAGCTTTCTGTCCGATGCCATGAAGTCCGAGGAGATCACAGCCCTGACCAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276301
Insert Size	2301 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276301.1, NP_001263230.1
RefSeq Size	3979 bp
RefSeq ORF	2301 bp
Locus ID	11717
UniProt ID	O08739
Gene Summary	This gene encodes a member of the adenosine and AMP deaminases family. The encoded protein is an AMP deaminase involved in nucleotide and energy metabolism in erythrocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) uses an alternate 5' exon, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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