Cdh26 (NM_001291189) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm1010
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228832 representing NM_001291189 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACCAGAGGCTGTGCCTGGTTGCTGCTGCTGCTGTCATTACCGCAGGGACAGAGCCACCAGCCTC TACATCGATCCAAGAGAAGATGGGTTCTCACCACCCTGGAACTGCAGGAGGAAGACCCAGGGCCCTTTCC TAAACTGGTTGGGGAGCTCTTCAATAATATGTCAAACAATGTGTCACTGATATATTTAATCAGAGGACCT GGTGTGGACGAATTTCCAGAGATTGGTTTATTCTCTATAGAAGATCACCAGAGTGGAAAAATATATGTTC ACCGCCCTGTCGATCGAGAAGTGACACCGTCTTTTATGGTGCACTTTGATGCCGTAGATCGATCCACAGG GAAAGTTGTGGATGAATCCCTGATTTTCAACATTAGAATCAGAGATGTGAATGACCATGCACCCCAGTTT CCTGAGAAGGAATTTAACATCAGTGTGAAAGAGAGCCAAGCCGCAGGTCAACCTATTTTTCAGTTGTTAA CGGTCGATTTGGACCAAGAAAACACTCCAAACTCTCAAGTCCTTTATTTCCTCGTCTCTCAAACGCCATT ACTGAGGGAAAGTGGCTTTCGGATTGATCTCATCAGCGGGGAAGTGCGACTCTCGGGATGCTTACATTAT GAGACTGCTCCTCTGTTCACACTGATCGTCAGAGCAAGTGATTGTGGAGAGCCATCCCTGACATCCACGG CCACCATTCATGTCAGTGTGGAAGACAGCAACAACCACATGCCCACATTTATGGAAGACCGTTATGAGAT TCGGATTTCTGAAGGTCAAGTCGAACAGGGTGTGTTGTATCTCCCTGTCCAAGATGGAGATTCTCCATTT ACACCAGCATGGAGAACACAATTCAACATATGGGATGGCAACGAGGAGGGCCATTTTGATATTGTGACTG ACCCTGAGACCAACCAAGGGTTGTTAAGTATTATCAAGCCTTTGGATTATGAATCTCAAGTAGCCCATAG CCTCGTCGTTGTTGTGGAGAATCAGGAGCAGCTCTTCGTTTGTACGGAGGGCCAGCTGCAGCCATTGAGG AAGGCGATGGCCAGCACCATGGTGAGCGTGCAGGTGCTGGACACCAATGACCCACCAGCCTTTCACCCTC AGAGCTTCATTGTCAGTGAGGAGGATGGAGCCTGGCCCGCCATCCAGCTGGGATACTTCAATGCTACAGA CCCAGACAGGGCTGACAGCCAGATAAGATACAAACTGGTTCATGATCCAGAAAATTGGGTGACCGTGGAT GAGAAGTCAGGAGTGGTGACCACCAAGAAGCAAATAGACCGGGAGTCCCCTCATGTGAATGACAGTTTCT ATACAATCATCGTCCATGCAGTTGACAACGGCCTTCCCCCGCTGACTGGCACGGGGACTCTGATGCTTTT CCTATCTGATGTCAACGACAATGCTCCAACCCTCCGGCCCCATTCCCGACACTTGGAGGTCTGTGAGTCG GCTGGGAGCCAGCCCCTGCTGATAGAAGCTGAGGATGCAGATCTGGACCCCTACGCTGACCCCTTTACTT TTGATTTGGACAATGCTCAGGGAGACGTGGAAGAGACTTGGATGCTGAGGACAAAGCAGGGGGAAGGCCA CTCTGCTGAACTCACAATGCTGAGAAGCGTCCCGCCCGGGGATTACTTGGTGCCACTCTTCATAGCTGAC AGACAGGGCCTCGCCCAGAAGCAGACAGTCCACGTGAGAATCTGCTCCTGCCGCAGTGGCTCTGAATGTG AGGAGCCTTCGGACACATGGCTCCTGTGGTGGGCCCTGTCCCCAGTTGGTGCAGCACTTATGGTTCTGTC AGCTGCTCTCCTTTGCCTGCTGCGGTGCTCCTGCACATTTGGACCCAAGAGGCTCAGAGGCTTCATCCCA AGTGACAGTGGCCACCAGACACTGATTGTGTATAACGAGGAAAGCAAAGTCCCTTCAGCCCAGGTCTATC TGGATCGTATGGTACCCAGACAGCAGCCCCTGCAGCTGCTGGAAGGAAGAGTGGTGGAGGCATGGAGCCA GAAGCTCCAGAGTATTGATGTTCTGGAAGGTGACACTGGCTACCTGCCTCATGTTTACAGAGAGGAGGGC GAGTGTGAAGGAGCTGAGACCCTCAGCTCTCTAACCTTCTTGGAGCAGGACCTCTCCCCCAAGTTGCTGG GCTGCTCGGGCTCAAAATCTACTCCCTCTGAAGCAATGTGTTTTACATCAAGAGTTCCTTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291189
Insert Size	2235 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291189.1, NP_001278118.1
RefSeq Size	2965 bp
RefSeq ORF	2235 bp
Locus ID	381409
UniProt ID	P59862
Gene Summary	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Ligand for integrins alpha-E/beta-7, ITGAE:ITGAB7, alpha-4/beta-7, ITGA4:ITGAB7 and alpha-4/beta-1, ITGA4:ITGAB1 through which modulates CD4(+) T cells activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

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