Gab1 (NM_001301298) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA408973; AW107238
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228782 representing NM_001301298 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCGGCGAAGTGGTTTGCTCGGGATGGCTCCGCAAGTCGCCCCCGGAGAAGAAGTTGAAGCGTT ATGCGTGGAAGAGAAGGTGGTTTGTGTTGCGCAGTGGCCGTTTGACTGGAGACCCGGATGTCCTGGAGTA TTACAAAAACGATCATGCCAAGAAGCCTATTCGGATTATTGATTTAAATTTATGTCAGCAAGTTGATGCT GGGTTGACATTCAACAAAAAGGAGTTTGAAAACAGCTATATCTTTGATATCAACACCATCGACCGGATTT TCTACTTGGTGGCAGATAGTGAGGAAGACATGAACAAGTGGGTCCGTTGTATCTGTGACATCTGTGGATT CAATCCCACAGAAGAAGATCCTGTGAAGCCGCTGACTGGCTCCTCACAAGCACCCGTCGATTCACCTTTC GCTATAAGTACAGCACCAGCCTCCAGTCAGATGGAAGCTTCTTCAGTCGCGCTACCTCCTCCTTACCAGG TCATCAGCCTTCCGCCACACCCAGACACCCTCGGCCTCCAGGACGATCCACAAGACTACCTCTTGCTGAT CAACTGTCAAAGCAAGAAGCCTGAACCTAACAGAACCCTCTTTGACTCTGCCAAGCCCACCTTTTCTGAG ACAGACTGCAATGACAACGTCCCTTCCCACCAGACTCCTGCTTCCTCCCAGAGCAAACACGGAATGAATG GCTTTTTCCAGCAACAAATGATGTATGACTGCCCACCGTCCCGGCTGACATCTGTCTCGGGAGAGTCCAG CCTCTATAACCTGCCCAGGAGCTATTCCCATGACGTGTTGCCAAAGGAATCCCCATCAAGCACGGAGGCC GACGGGGAGCTGTACACCTTTAACACCCCATCTGGGACTGCAGGTGTAGAAACGCAGATGAGACATGTAT CCATCAGTTACGACATTCCGCCAACACCTGGCAACACTTACCAGATCCCACGGACATTTCCAGAAAGCAC ACTGGGACAGTCATCAAAGCTGGACACCATTCCTGATATCCCCCCACCTCGGCCACCAAAGCCACATCCA ACTCATGACCGGTCTCCTGTGGAAACGTGTGGAGTCCCACGCACGGCCTCGGACACTGACAGCAGTTACT GTATCCCTCCTCCAGCAGGCATGACGCCCTCCCGGAGTAATACCATTTCCACCGTGGATTTGAACAAGTT GCGGAAAGATGCTAGTTCTCAAGATTGCTATGATATTCCACGGACCTTTCCGAGCGATAGATCTAGTTCC CTGGAAGGCTTCCATAGCCAGTATAAAATCAAAAGCGTGTTGACAGCGGGAGGTGTCTCGGGTGAAGAGC TGGATGAGAACTACGTTCCCATGAACCCCAACTCGCCACCTCGACAACATTCCGGCAGCTTTACCGAGCC AATCCAGGAGCCAAACTATGTGCCAATGACCCCAGGGACCTTTGACTTTTCTTCCTTTGGAATGCAAGTC CCTCCTCCTGCTCATATGGGCTTCAGGTCCAGCCCAAAGACCCCTCCCAGGAGGCCAGTTCCTGTTGCTG ACTGTGAACCACCCCCGGTGGATAGGAACCTCAAGCCAGACAGAAAAGGTCAGAGTCCTAAAATTTTAAG ACCCAAACCCCATGGTTTAGAGCGAACTGATTCACAAACCATAGGTGACTTTGCTACAAGAAGAAAGGTC AAGCCGGCACCTTTAGACATAAAACCTCTGTCAGAATGGGAAGAGCTGCAAGCCCCAGTCAGATCTCCCA TCACCAGGAGCTTCGCTCGGGACTCCTCTAGGTTTCCCATGTCCCCTCGGCCTGATTCTGTGCACAGTAC GACATCGAGCAGCGACTCTCATGACAGTGAAGAGAACTATGTCCCCATGAATCCAAATCTGTCTGGCGAA GACCCGAATCTCTTTGCCAGCAACAGCCTTGATGGGGGAAGCAGCCCGATGAATAAACCCAAAGGAGACA AACAAGTCGAATACCTGGATTTAGACCTAGATTCTGGGAAGTCCACGCCACCACGGAAGCAAAAGAGCAG TGGTTCTGGCAGCAGCATGGCAGACGAGAGGGTGGATTACGTTGTGGTGGACCAACAGAAGACTCTGGCC CTGAAGAGTACCAGAGAAGCTTGGACGGATGGGAGGCAGTCCACAGAGTCCGAGACACCCACCAAGAATG TGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301298
Insert Size	2178 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301298.1, NP_001288227.1
RefSeq Size	5006 bp
RefSeq ORF	2178 bp
Locus ID	14388
Gene Summary	Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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