Cpeb3 (NM_001290828) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4831444O18Rik; CPE-BP3; mKIAA0940
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228696 representing NM_001290828 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGATGATTTACTGATGGACAAAAGCAAAACCCAGCCCCAGTCTCAGCAGCAGCAGCGGCAGCAGC AGCAGCAGCAGCAACAGCTCCAGCCCGAGCCCGGCGCAGCTGAAGCCCCGTCCACGCCCCTCTCCTCAGA GATCCCCAAGCCCGAAGACAGTAGCGCAGTGCCGGCCCTCAGCCCCGCCTCGGCTCCGCCAGCCCCCAAC GGCCCCGACAAGATGCAGATGGAGTCGCCGCTCCTGCCGGGCTTGAGTTTCCATCAGCCCCCTCAGCAGC CGCCGCCGCCGCAGGAGCCCACGGCGCCCGGAGCGTCGCTGTCGCCGTCCTTCGGCAGCACCTGGTCCAC AGGCACTACAAACGCGGTGGAGGACAGCTTCTTCCAGGGGATCACCCCAGTCAACGGGACCATGCTCTTC CAAAACTTCCCGCACCACGTCAACCCGGTCTTCGGAGGCACCTTCTCGCCGCAGATCGGCCTGGCGCAGA CCCAGCACCATCAGCAGCCTCCACCGCCCGCGCCGCAGCCGCCGCAGCCCGCGCAGCCCCCGCAGGCGCA GCCCTCGCAGCAACGCCGCTCGCCCGCCAGCCCCAGCCAGGCGCCCTATGCGCAGAGGAGCGCCGCTGCC TACGGCCACCAGCCCATCATGACCAGCAAGCCGTCCTCATCCTCGGCGGTCGCGGCTGCTGCGGCCGCGG CCGCTGCCTCTTCGGCCTCGTCCAGCTGGAACACGCACCAGAGCGTGAACGCCGCCTGGAGTGCTCCGTC CAACCCGTGGGGTGGCCTGCAGGCGGGCCGAGACCCTCGCCGCGCGGTCGGCGTGGGCGTGGGTGTAGGT GTGGGGGTACCCTCCCCGCTTAACCCCATCTCGCCGCTCAAAAAGCCCTTCTCCAGCAATGTGATCGCTC CACCCAAGTTCCCTCGTGCAGCCCCGCTCACCTCCAAGTCCTGGATGGAGGATAACGCTTTCCGGACCGA TAATGGTAACAATCTGTTGCCTTTTCAGGACCGGAGTAGGCCCTATGACACTTTTAACTTGCACTCCTTG GAGAACTCCTTAATGGATATGATAAGGACTGACCATGAGCCTCTGAAAGGACGCATGGGAATAAATTTCC ACCATCCAGGAACAGATAACATTATGGCACTTAACACCAGGAGCTATGGGCGGAGACGAGGTCGGTCTTC TCTCTTTCCCTTTGAAGACGCCTTCTTGGATGACAGCCATGGTGATCAGGCCTTATCGTCTGGCTTAAGT TCTCCTACTCGATGTCAAAATGGGGAACGAGTGGAACGCTACTCTAGAAAGGTGTTTGTTGGAGGCCTTC CTCCTGACATCGATGAAGATGAGATCACTGCCAGCTTTCGCAGGTTTGGACCCCTTGTGGTGGACTGGCC TCATAAAGCCGAGAGCAAATCGTACTTCCCTCCTAAAGGCTATGCTTTCCTGCTGTTCCAGGAGGAGAGC TCAGTGCAAGCTCTGATAGATGCCTGCCTGGAGGAGGATGGGAAACTGTACCTGTGTGTGTCCAGCCCAA CCATCAAGGATAAACCGGTGCAAATCCGACCGTGGAACCTAAGTGACAGTGACTTTGTAATGGATGGCTC TCAGCCTTTGGACCCCAGAAAAACTATCTTTGTTGGGGGAGTTCCACGACCCCTTCGAGCTGTTGAACTG GCAATGATAATGGACCGTTTGTACGGTGGTGTTTGCTATGCTGGCATTGACACAGACCCAGAGCTGAAGT ACCCGAAAGGTGCTGGGCGTGTTGCGTTCTCCAATCAGCAGAGCTACATTGCAGCCATCAGTGCGCGCTT TGTGCAACTTCAACACAACGACATTGACAAACGGGTGGAAGTGAAGCCGTACGTGCTGGATGATCAGATG TGTGACGAGTGTCAGGGCACACGCTGCGGGGGGAAGTTTGCCCCATTCTTCTGTGCCAACGTCACCTGTC TGCAGTACTACTGTGAATACTGCTGGGCCAGCATCCACTCCCGAGCCGGCCGTGAGTTCCACAAACCGCT GGTGAAGGAGGGAGGCGACCGCCCCCGGCACGTCCCGTTCCGCTGGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290828
Insert Size	2082 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290828.1, NP_001277757.1
RefSeq Size	5901 bp
RefSeq ORF	2082 bp
Locus ID	208922
UniProt ID	Q7TN99
Gene Summary	Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (PubMed:17024188, PubMed:26074072). In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3' UTR, but binds to a U-rich loop within a stem-loop structure (PubMed:17024188). Required for the consolidation and maintenance of hippocampal-based long term memory (PubMed:26074003). In the basal state, binds to the mRNA 3' UTR of the glutamate receptors GRIA1 and GRIA2 and negatively regulates their translation (PubMed:17024188, PubMed:22153079). Also represses the translation of DLG4, GRIN1 GRIN2A and GRIN2B (PubMed:24155305). When activated, acts as a translational activator of GRIA1 and GRIA2 (PubMed:22153079, PubMed:26074003). In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (PubMed:26074071). Binds to the 3' UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (PubMed:26915043). Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (PubMed:26074072). Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (By similarity). In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (By similarity). In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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