Nphp1 (NM_001291013) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228683 representing NM_001291013 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGCCCGGCGGCCGCGGGACCCGCTGCAGGCGCTGAGGCGCCGCGGGCAGGAGCTGAAGCTGCAGG TTGATAGTTTGGTGACTGAAAGCCAACTAACAGGTGCTCTGGAACCGAGTAAAAGGAGAGAGATTTACCA AAGATGTATCCAGTTAAAACAGGCAGTAGATGAAAATAAAAACACCCTTCAAAAATTAAACAAAGCTGAT GAAGCTGCACCTGTGGGGAACTATGAGCAGAGGAAGGAGGAGGAACACAGCCTTCTGGAGAAGCTAGCCT GCCAGCTGCAGGAGCTTGCCGTGAGCATCAGCAGGAAAGACGCTCTCAAAGTTGAGGCACACAGTGACAA GGAGGAAGATGACACGACTGAAGATGATGAAGAAGAAACTGGTGGAGAGGAAGAAGAGTCAGAAGGGGAT GGAGAAGGAAAGCAAGAGCAAGCCTCCCCGAAACAAGCAGAAACAGAAACTGTCACATACATCGCTCTTG GGGACTTCGCTGCTCAGCAGACTGGGGACTTGACCTTTAAGAAAGGGGACGTTCTCCATATAATTGAAAA GAAGCCTGATGGTTGGTGGCTGGCTAAGGATGCTGAAGGAGTTGAGGGTCTCATTCCCAGGACCTACTTG GAGCCCTATAATAAAGAAGACAAGCTGGAGTCAAGCGAAGGCAGCGAGGAGGGCGGTGAGGAGGACGGTG AGGAGGACGGTGAGGAGGACGTGGAGGTGGTGGATGAAACTGCAGATGGAGCCCAGGTGAAGCAGAGAAC TGATTCCCACTGGAGTGCTGTACGAAAAGCTATCTCAGAGATAAATACCGTTGATGTGTTAGCCACAATG GGAGCCATTCCTGCAGGATTCCGGCCTTCCACGCTCTCCCAGCTGCTGGATGAAGGGAACCAGTTTCGAG CAAGTTACTTCTTGCAGCCAGAGCTCACGACATCGCAGCTGGCCTTCAGAGATCTAACATGGGATGCTAA GGCAGGCACGATCATGTCGAGACCAAGTCGTGTTTCATTGATTCTGACCCTATGGAGCTGTAAAATGATC CCTCTCCCAGGAACAAGCATACAGGTTCTCAGCAGACACATCCGCCTCTGCCTGTTTGACGGCAGCAAGG TCCTGAGTAATATCCATACAGTTCGAGCCGTGTGGCAGCCTAAGAAGCCAAAAACATGGACCTTCTCTCC TCAGGTCACTGGCATCTTGCCATGCTTGCTCGATGGTGACTGTTTTATCAGGTCCAATTCTTCAACTCCA GACCTTGGGATATTATTTGAACTTGGAATTTCTTACATTCGCAATTCAACTGGTGAGAGAGGAGAGTTAA GCTGTGGCTGGGTGTTTCTTAAGCTTTTCGATGCCAGTGGAGTTCCTATTCCAGCAAAAACATACGAACT CTTCTTGAATGGCGGCACTCCTTACGAAAAAGGTGTTGAAGTGGACCCTTCAGTGTCCAGAAGAGCACAG GGCAGTGTTTTCCGTCAGATGATAAGTGTGCGCAGGCAGCCTCAACTTCTGGTGAAACTTCGGTCTCTGA ACAGGAGGTCCAGGGCCATGCTCAGTCTGCTGCCAGAAACCCTGGTGGGGAGTATGTGCTCTGCTCACTT ACTGATATTTTACCGACAAATTCTTGGAGATGTGCTCCTGAGAGACAGGACAAACCTGCAAAGTGCCGAT TTAATCAGTCATCCCGTGCTGGCCACCTTCCCTCTGCTCCTGGAGCAGCCCGACGTGATGGATGCTCTCA GGAGTTCCTGGTCTGAGAAAGAAAGTACACTAAAGCGAGCAGAGAAGAGAGACAAAGAGCTCCTAAAGGC TGAGTTCCTCCTGGTCTACCATGACTGCGTGCTCCCTCTTCTGCACTCGACACTGCTGCCCCCCTTTAGG TGGGCCGAGGAAGAGACAGAGGCTGCAAGATGGAAAGCCATTGCTGACTTCCTCAAGCAGAGCCGGGAGA ACGAGGGTTCCCTGAAGGCTCTGCTGTCACCAGATGGAGTTCACAAACCATTTGACCTTTCGGAGCAGAC CTTTGACTTCTTGGGTGAAATAAGAAAGAACTCAGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291013
Insert Size	2070 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291013.1, NP_001277942.1
RefSeq Size	2297 bp
RefSeq ORF	2070 bp
Locus ID	53885
Gene Summary	Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells, together with NPHP4 and RPGRIP1L/NPHP8 (PubMed:21565611). Does not seem to be strictly required for ciliogenesis (PubMed:19208653). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling (PubMed:11493697). May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly (PubMed:19208653). Required for normal retina development (PubMed:19208653). In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19 (PubMed:19208653). Involved in spermatogenesis; required for the differentiation of early elongating spermatids into spermatozoa (PubMed:18684731).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (c) that is 2 aa shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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