Tmem232 (NM_001289485) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E130009J12Rik; Gm940; Tes13-L; Tes13-S
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228640 representing NM_001289485 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCTTATAAGCCACCTGTGGTCAATAAGTTTGGAGTAATATCTTCCACTTATCATGAAGAACTCC TGAAATCTATTTTTGAAAGTTCAAACAGAAGAAAGAGTCAAAAACCAAAGCCATCATTTTCAATTTCCAA AGAATTCATATTGAGATTCAACCACACAGATAATCCAGCAGAGGAGGAAGAACTGTTGGAACAAGCCAGG AGACTCATCGTCAGGTCTAAGAGGAAGTTGGGCCTCAAGACACTGGGCTCTGGGAAGCATGTGCATCTTC CGACCGCATGGGCTGAGGTCATCTATCTGGCTCAGTGCAAAGGGGAGATCCAAGATGAAGCTTTAAATAT GCTCCACGCCTCCTTGGACCATGTCTCCTTTGATCACGATCAGCTTCCTGCCCTGTTTTTTCTCGCGGAA TCCGTTTTATATCGACTCTGTTGCGATGCATTCATGAAGGGATATTTATATTCAGTTGAGATAAAACTGG TGAAGATTGGCTATTTGATCTTCTTACGGCTTTTTGTATTCTTCCTGCATGGTCACTTAGAAAGCTTTAA ACAACATTTGCTTAGGCTGCAGCCATATTTATACGCACTTCATTTCTCGGAACCGTCCTACTACAAGTAT CCGAACATCATCTCCAACGTGCAGTTCATCCTGAAAACATCGGAAATTATCTGTAAAAGAGAGCTTCATT CTGAACCATTCGTGGAAAGCCCAGATGAAACTGAGGATCCATATTCTGACCTGAATCATTTGCAGCTGAA TAAGAGGGGCTACGAAGTCAACCACCTGCTCTGGCACAGTGTGGCCGCCTGGTCCTGCGTTCAGAACAAT CGTCCTCAGCTCACCGAAGTGCTGGAACACCTCCTCTTCTACAAAACGCAGCTTCAGACGAAATGCTGGT TGGATTCAGCCCTGGCTTTGATGGTCCTGGGGGAAGCTGCTAAGCTGGACATGGCCTGCTTGAAAACACT GATGGACCTAGTGACAGATTTCCTTGAGAACATCTTGTCTGCTCAGAACCAGGAAGAGAACTATAATATA TATGATACTTCCTGGGCCTCAGAGATTGTATTCACATATACAACAATTATTGCAGAAGTCTGTCTGTATG CAGCTACCTCTGACCTGAGGAAAACAGCTTTGATTGGGTTCTGTGCCTGTAAAAGTCCGCAGCAAGGTAT TTCCCTCACAGACAAGTCTGAAGAGCTGCCAGAGTTGGATGGCGCAAGCATTTTAACTCTTCTGAAGTAT TTCTCTTCCAGAATCTCTGATAACTGTGAGAAGGTGATCTGGATTGGATACTATGGCATAGTGTATAACC TGGTGAAGATGTCATGGGAACTTCAGGGTGAGCAGGATCAGGATGGACTTAGAAACATGATATGGCAAAC ACTGCAAAAGATAAAGGATTATGAGCAAGACCCACGGATCCGATGTGCATTAGTTATAGCCCAGGCCGAA TTAAATGGCCCATCTGACCCTTTCTGCACAAAGGCCACACCAAACTCAGGAGAAGAAGTTTTCTCCAAGT ACATCGGGTGGAGAATCGCTACTACTCTCTCCAGACTCTTTTTCCCCTCTCTCGATGTTGCCCCTCCAAA GACGCCAGTGGAAGTGGACCTGCCAAGAAAACACACCATCAGAGAACGCCAGCCTGCAAAAAAGAGAGTT CTGCGCTTTATCTTGAAGGATCATTCTTCTGTGGTAGAAGTTTCCATGACTCCGTATCCAAATTTCTTCA CCAAGGCAGATAAGAAATTGGAAGAAATCATTGATCATCATTGGCAGAAAGATATGGAGGCCCGAAAGCG AGAAGAGGAAGCATATAAGGCCCAAAATCAGAAGGACAAGGAGGAAAAGGAGAAGATTCACTTTCAAGAA ATTATGAAGCAAAGAGAACGGAAACTGAACAAACAAACCAAACCCTATGAAATAATTCTGTCAGAAAAAG AAAGTGGCTCAGAAAAGAAATGTGGGTTCTTCGAGCTGAAACCCAGCACCGCACCAAATGCTAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289485
Insert Size	2028 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289485.1, NP_001276414.1
RefSeq Size	2679 bp
RefSeq ORF	2028 bp
Locus ID	381107
UniProt ID	Q5K6N0

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