Alas1 (NM_001291835) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ALAS; Alas-1; Alas-h; ALAS-N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228556 representing NM_001291835 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGTCGTTCGCCGATGCCCATTCTTATCCCGAGTCCCTCAGGCCTTTCTGCAGAAGGCAGGAA AGTCTCTGTTGTTCTATGCTCAAAACTGCCCCAAGATGATGGAAGTTGGGGCCAAGCCAGCTCCTCGAAC CCTGTCCACATCAGCTGTCCACTGCCAGCAGGTCAAAGAAACCCCTCCAGCCAATGAGAAAGAGAAAACT GCCAAGGCCGCAGTCCAGCAGGCTCCTGACGAGTCCCAGATGGCACAGACTCCAGACGGCACACAGCTTC CGTCTGGACACCCGTCCCCTGCTACAAGCCAGGGCTCTGGGAGCAAGTGCCCTTTCCTGGCAGCACAGCT GAGCCAGACAGGCAGCAGCGTCTTCCGCAAGGCCAGTCTGGAGCTCCAGGAGGACGTGCAGGAAATGCAT GCTGTGAGGAAAGAGGCTGCTCAAAGCCCAGTGCCTCCCAGCCTGGTTAATGTGAAAACCGATGGAGAGG ATCCAAGCCGACTGCTGAAGAACTTCCAGGACATCATGCGAAAGCAGAGGCCAGAAAGAGTGTCTCATCT TCTTCAGGATAACTTGCCAAAGTCTGTTTCCACTTTTCAATATGATCATTTCTTTGAGAAGAAAATTGAT GAGAAAAAAAATGATCATACCTACCGAGTTTTTAAAACGGTGAACCGGAGAGCACAAATCTTCCCGATGG CGGATGACTACACGGATTCCCTCATAACCAAAAAGCAGGTGTCGGTCTGGTGCAGTAATGACTACCTAGG CATGAGTCGACACCCACGGGTGTGTGGGGCTGTCATGGAGACTGTGAAACAGCATGGTGCTGGAGCTGGG GGAACTAGGAATATTTCTGGAACGAGCAAGTTCCACGTAGAACTGGAGCAGGCACTGGCCGACCTCCACG GCAAAGATGCGGCGCTCTTGTTCTCTTCCTGTTTTGTGGCCAACGACTCCACCCTCTTCACCCTGGCTAA GATGATGCCAGGCTGTGAAATTTACTCTGATTCCGGGAACCATGCCTCCATGATCCAAGGGATTCGCAAC AGTCGAGTGCCAAAATATATCTTCCGCCACAATGACGTTAACCATCTCAGAGAACTGCTGCAGAGATCTG ACCCCTCAGTCCCCAAGATAGTAGCATTTGAAACTGTCCATTCAATGGATGGAGCAGTGTGCCCACTGGA AGAGCTGTGTGACGTGGCCCATGAGTTTGGGGCAATCACGTTTGTGGATGAGGTCCATGCAGTGGGGCTC TATGGAGCTCGAGGTGGAGGGATCGGTGATCGGGATGGCGTCATGCCAAAAATGGACATCATCTCTGGGA CGCTTGGTAAAGCGTTCGGCTGTGTTGGAGGATACATTGCCAGCACGAGTTTGCTGATCGACACCGTCCG CTCCTACGCTGCGGGCTTCATCTTCACCACCTCCTTGCCACCAATGCTGCTGGCTGGAGCCCTGGAGTCT GTGCGGATCCTGAAGAGCAGCGAGGGGCGCGCCCTTCGCCGCCAGCACCAGCGAAATGTCAAGCTTCTGA GGCAGATGCTAATGGACGCTGGCCTCCCGGTCATCCACTGTCCGAGTCACATCATCCCTGTGCGGGTTGC CGATGCTGCTAAGAACACAGAGATCTGTGATGAGTTGATGACCAGGCATAATATCTACGTCCAGGCCATC AATTACCCAACAGTGCCTCGCGGGGAGGAGCTCCTGCGGATCGCCCCCACCCCGCATCACACCCCGCAGA TGATGAACTTCTTCGTGGAGAAGCTGCTGGTCACGTGGAAGCGAGTTGGGCTGGAACTTAAGCCACATTC GTCAGCTGAATGCAACTTCTGCAGGAGGCCCCTGCACTTTGAAGTGATGAGCGAAAGAGAGAAAGCCTAT TTCTCAGGCATGAGCAAGATGGTGTCTGCCCAGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291835
Insert Size	1929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291835.1, NP_001278764.1
RefSeq Size	3089 bp
RefSeq ORF	1929 bp
Locus ID	11655
UniProt ID	Q8VC19

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