Sgip1 (NM_001285862) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110007P09Rik; gag; SGIP1alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228454 representing NM_001285862 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAAGGACTGAAAAAACGTACAAGGAAGGCCTTTGGAATACGGAAGAAAGAAAAAGACACTGACT CTACAGGCTCGCCAGACCGAGATGGAATGAAGAAAAGCAATGGGGCACCAAATGGATTTTATGCAGAAAT CGATTGGGAAAGATATAACTCACCTGAGCTAGATGAAGAAGGTTACAGCATCAGACCTGAGGAACCAGGC TCTACCAAAGGAAAGCACTTTTATTCCTCAAGTGAATCTGAAGAGGAAGAAGAGTCGCACAAGAAATTCA ACATCAAGATTAAACCCTTGCAATCTAAGGACGTCCTGAAGAATGCAGCGACGGTAGATGAGCTGAAGGC TTCAATAGGCAACATCGCACTTTCCCCTTCGCCTGTGGGTGCAATTAAAAGGAACTTATCCAGTGAAGAA GTTGCAAGACCCAGGCGTTCCACCCCAACTCCAGAACTTACAAGCAAGAAGCCTCTGGATGACACTCTGG CCCTTGCTCCCCTCTTTGGTCCACCATTAGAGTCAGCTTTTGATGAGCAGAAGACGGAAGTTCTTTTAGA TCAGCCTGAGATATGGGGTTCAGGCCAACCGATGAACCCAAGTACGGAGTCGCCAGAGCTAGCAAGACCT TTCCCCACTGGAACACCTCCACCTCTGCCTCCAAAAACTGTACCAGCCACTCCGCCTCGAACAGGCTCCC CCTTAACAGTTGCAACAGGTGCGTCTTCTCCTGCTCGACCAGCCACCCCCTTAGTTCCTTGCAGCACCAC TCCGCCTCCACCTCCTCCCCGGCCTCCATCCCGGCCAAAGCTACCTCCAGGAAAGCCTGGAGTTGGAGAT GTGTCCAGACCTTTTAGCCCACCCATACACTCCTCCAGCCCTCCTCCGATAGCACCCTTAGCCCGGGCTG AAAGCACTTCTTCAATATCGTCAACTAACTCACTGAGCGCAGCCACCACTCCCACAGTTGGTTCCTCCCG GGGGCCCAGTCCCCTAACTATGGGGGCCCAGGACACCCTCCCGGTCGCAGCAGCTTTCACAGAAACTGTC AACGCCTACTTCAAAGGAGCAGATCCAAGCAAGTGCATTGTTAAGATCACGGGAGAAATGGTGTTGTCCT TCCCTGCTGGTATCACCAGACACTTTGCCAACAACCCATCCCCAGCAGCTCTGACTTTCCGGGTGGTAAA TTCCAGCAGGTTAGAGCACGTCCTGCCGAACCCCCAACTTCTCTGCTGTGATAATACACAAAATGATGCC AATACCAAGGAATTCTGGGTAAACATGCCAAATTTGATGACTCACCTAAAGAAAGTCTCTGAACAAAAAC CCCAGGCTACATACTATAATGTGGACATGCTCAAATATCAGGTGTCTGCCCAGGGCATTCAGTCCACACC TCTGAACTTGGCGGTGAACTGGCGCTGTGAGCCTGCGAGCACTGACCTGCGCATAGACTATAAGTACAAC ACGGATGCCATGTCCACTGCAGTGGCCCTCAACAACGTGCAGTTCCTGGTCCCCATAGACGGAGGAGTGA CCAAGCTTCAGGCTGTGCTTCCTCCAGCAGTCTGGAATGCTGAACAACAAAGAATATTATGGAAGATTCC CGATATCTCCCAGAAGTCAGAAAATGGAGGCGTAGGTTCTTTACTGGCGAGATTTCAGTTATCCGAAGGC CCGAGCAAACCTTCCCCGCTGGTTGTGCAGTTCACGAGTGAAGGAAGTACTCTCTCTGGCTGTGACATTG AACTTGTTGGAGCAGGCTACCGGTTTTCACTCATTAAGAAGAGGTTTGCTGCAGGAAAGTACTTGGCAGA TAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285862
Insert Size	1827 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285862.1, NP_001272791.1
RefSeq Size	4851 bp
RefSeq ORF	1827 bp
Locus ID	73094
UniProt ID	Q8VD37
Gene Summary	May function in clathrin-mediated endocytosis. Has both a membrane binding/tubulating activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a preference for membranes enriched in phosphatidylserine and phosphoinositides and is required for the endocytosis of the transferrin receptor. May also bind tubulin. May play a role in the regulation of energy homeostasis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks six alternate in-frame exons among the 5' and central coding regions, compared to variant 1, resulting in an isoform (e) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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