Cdc7 (NM_001271566) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI597260; Cdc7l1; muCdc7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228320 representing NM_001271566 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCCAATGGCGTTTTCTTCCCTTCGTGGCAGTGACAGGTGTCCGGCTGATGACTCTTTAAAAA AATATGAGCAGAGTGTTAAACTTTCAGGTATTAAAAGAGATATTGAGGAGCTTTGTGAAGCTGTACCACA ACTAGTCAATGTGTTCAAAATTAAGGACAAAATTGGAGAAGGCACCTTCAGCTCTGTTTATTTGGCCACA GCACAGTTGCAAGAAGGACATGAAGAGAAAATTGCGCTGAAACACTTAATTCCCACAAGTCATCCTATGA GGATTGCAGCAGAGCTTCAGTGTCTGACAGTTGCAGGGGGGCAAGACAATGTCATGGGACTTAAGTACTG CTTCAGAAAAAATGATCATGTGGTTATTGCTATGCCGTATCTGGAACATGAGTCCTTTTTGGACATTTTG AATTCTCTTTCCTTCCAAGAAGTTCGGGAATATATGTATAATCTTTTTGTAGCTTTGAAACGGATTCATC AGTTTGGTATTGTTCACCGTGATGTGAAGCCCAGCAATTTTTTATACAATAGACGTCTGAAAAAGTATGC CTTGGTGGACTTCGGTTTGGCCCAGGGAACCCGTGACACAAAAATAGAGCTGCTCAAGTTTGTCCAGTCT GAGGCTCAGCAGGAAGATTGTTCACGAAACAAGTATCATGGAGTCGTTGGACACAAGGGCCTGCTGAGTC GCCCGGCACCTAAAACTGTGGATCAGCAGTGTACCCCAAAAACTTCTGTCAAAAGATCCTACACACAAGT TCACATTAAGCAAGGAAAAGACGGAAAGGAGAGATCTGTAGGCCTTTCTGTCCAGCGCTCTGTTTTTGGA GAGAGAAATTTCAATATACACAGCTCCATTTCACATGAGAGCCCTGCAGAGAAACTCATCAAGCAATCAA AGACTGTGGACATAATATCACGAAAGCTAGCGACAAAAAAGACGGCCATTTCTACAAAAGCTATGAACAG CGTGATGAGGGAAACTGCCAGGTCCTGCCCTGCTGTCCTCACCTGCGACTGCTATGGATCAGATAGAGTC TGCAGCGTTTGCCTGTCGAGGCGGCAGCAGGTTGCCCCTAGGGCAGGCACACCAGGATTCAGAGCGCCAG AGGTCCTGACAAAGTGTCCTGACCAGACCACAGCGATTGACATGTGGTCTGCAGGTGTCATATTCCTGTC CTTGCTCAGTGGGCGGTACCCATTTTACAAGGCCAGTGATGACTTAACTGCTTTGGCTCAGATCATGACA ATTCGAGGATCCAGGGAAACTATCCAGGCTGCTAAAGCTTTTGGCAAATCAGTTCTGTGTAGCAAAGAAG TCCCAGCACAAGACTTGAGAGCTCTCTGTGAGAGACTGCGGGGTCTAGACTCTACCACTCCCAGGTCAGC CAGTGGTCCTCCAGGGAATGCTTCCTATGACCCTGCTGCTTCCAAGAACACTGACCACAAAGCATCCCGT GTACAGGCTGCTCAGGCACAGCACTCAGAGGACTCCTTGTATAAAAGGGACAACGATGGCTATTGGAGTC ATCCCAAAGACTGCACTTCCAACTCAGAAGGCTGGGACTCAGTACCTGATGAAGCCTATGACCTGCTCGA CAAGCTTCTGGACCTAAACCCAGCTTCAAGGATAACAGCAGAAGCAGCCTTATTACATGCGTTCTTTAAA GATATGTGCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271566
Insert Size	1695 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271566.1, NP_001258495.1
RefSeq Size	3137 bp
RefSeq ORF	1695 bp
Locus ID	12545
UniProt ID	Q9Z0H0
Gene Summary	Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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