Rad17 (NM_001283011) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MmRad24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227905 representing NM_001283011 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGGAGACGATCTGACAACTGATAAGAAGATCATTCTGGTGGAAGAGTTACCTAACCAGTTTTATC GAGACCCTAACGCTTTACATGAGATTCTAAGAAAGCATGTGCAGATTGGCCGGTGTCCTCTTGTATTTAT AGTCTCTGACAGTGTCAGTGGAGACAATAATCAGCGGCTACTCTTCCCCAGAAACATTCAAGAAGAGTGT TCTGTTTCAAACATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATTGTGA CTATAGAAGCTAGTAAGAATGGAGAAAAAATTATTGTTCCTAATAAAACTTCTCTGGAGTTGCTCTGTCA AGGATGTTCTGGTGATATCAGAAGTGCAATAAACAGCCTGCAGTTCTCGTCTTCAAAAGGAGAGAACAGT TCGTGGTCAAAGAAGAAAAGAATGTCATTGAAATCGGATGCTGCCATATCAAAATCAAAACAAAAAAAGA AACATAATAGTACTTTGGAAAATCAAGAGATTCAAGCTATTGGTGGAAAAGATGTTTCTCTTTTTCTCTT CAGAGCTTTGGGGAAGATTCTGTACTGTAAAAGAGCGCCTTTAACAGAGTTAGACTCACCTCGCCTGCCT GCTCACTTGTCAGAGCACGACCGGGACACTTTACTTGTTCAGCCTGAGGAAATAGTAGAAATGTCACACA TGCCAGGAGACTTTTTTAATTTATACCTTCACCAAAACTACATAGATTTCTTCGCTGAAGTAGATGATCT TGTGCCAGCCAGTGAGTTTCTGAGTTTTGCAGATATCCTCGGTGGTGATTGGAATACACGCTCGTTACTC AGGGAATATAGTACATCCGTAGCTACAAGAGGTGTGATGCATTCCAACAAAGCCCGGGGGTTCGCTCACT GCCAAGGAGGATCAAGCTTTCGACCCTTACATAAGCCCCAGTGGTTTCTAATACAGAAAAAGTATCGGGA AAATTGCCTGGCAGCAAAAGCACTCTTTGTTGACTTCTGCCTACCAGCTTTGTGTCTCCAGACTCAGCTC TTGCCATATCTTGCTCTTCTGACCATTCCAATGCGAAATAAAGCTCAGATTTCTTTTATCCAAGATGTTG GAAGACTTCCTTTGAAGCGAAGCTTTGGAAGGTTGAAAATGGAAGCCCTGACTGACCGAGAGCTGGGGCT GATAGACCCTGACAGTGGGGATGAGTCCCCGCACAGCGGAGGGCAGCCGGCTCAGGAAGCTCCCGGTGAA CCCGCGCAGGCTGCTCAGAATGCCGACCCAGAGACCTGGTCCCTTCCGCTGAGTCAGAACAGCGGGAGCG ATCTGCCTGCCAGCCAGCCTCAGCCCTTTTCCTCCAAAGTGGACATGGAAGAAGAGGAGGAGGAAGAGGA AGACATAATAATAGAGGACTATGACAGCGAGGAGACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001283011
Insert Size	1440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001283011.1, NP_001269940.1
RefSeq Size	2940 bp
RefSeq ORF	1440 bp
Locus ID	19356
UniProt ID	Q6NXW6
Gene Summary	Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination (By similarity). Essential for embryonic development. May be involved in homologous recombination.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate exon in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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