Cnot6l (NM_001285514) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4932442K20Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227781 representing NM_001285514 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTCTCAGGGAATTGCTTTTAAATGACAATTATTTACGGGTGTTGCCTTATGAACTTGGCCGGC TCTTCCAGCTACAAACTCTAGGTTTAACAGGCAATCCTTTATCACAGGATATTATGAGCTTATACCAGGA CCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAATCTTGCAGTTCATCCAGAGCAGCTTCCT CCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTGCCATCAGCATCATTCACGGTTATGTGTT ACAATGTGTTATGTGATAAATATGCTACCAGGCAGCTATATGGCTATTGTCCGTCCTGGGCATTAAACTG GGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAACTGGGACGCAGATATCATTAGTCTTCAGGAA GTGGAAACAGAGCAATACTTTACTCTCTTTCTGCCAGCATTGAAGGATCGTGGATATGATGGATTTTTTT CTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAAAGAAAGCATGTGGATGGTTGTGCAATATTCTT CAAAACAGAAAAATTTACATTGGTGCAGAAGCATACAGTGGAATTCAACCAGGTAGCAATGGCAAATTCA GATGGATCCGAAGCAATGCTAAACAGAGTAATGACGAAAGATAACATTGGCGTTGCTGTGGTGTTAGAGG TCCACAAGGAGCTTTTTGGAACAGGTATGAAGCCTATTCATGCTGCAGACAAACAGCTGCTTATAGTGGC AAATGCCCACATGCATTGGGACCCAGAGTATTCTGATGTGAAACTTATTCAGACCATGATGTTTGTCTCA GAGGTTAAAAACATTCTGGAGAAAGCCTCAAGTAGGCCTGGCAGCCCAACTGCAGATCCCAATTCCATCC CGCTGGTGCTATGTGCAGATCTTAACTCATTGCCAGATTCAGGTGTTGTGGAATATTTAAGCAACGGTGG AGTAGCTGACAACCATAAAGACTTCAAGGAACTAAGGTACAATGAGTGTCTTATGAACTTCAGCTGTAGT GGAAAGAATGGAAGCTCAGAAGGGAGAATCACACATGGCTTCCAACTTAAGAGCGCCTATGAAAATAACT TGATGCCTTATACCAATTACACCTTTGATTTCAAAGGTGTGATTGACTACATTTTCTATTCCAAGACTCA TATGAACGTGCTTGGTGTCCTGGGGCCTTTAGATCCTCAATGGCTGGTTGAGAACAACATCACTGGGTGT CCACACCCTCACATCCCTTCAGACCACTTCTCACTGTTAACACAACTTGAACTCCACCCTCCACTCCTGC CTCTTGTCAATGGTGTTCACTTGCCTAATCGGAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285514
Insert Size	1368 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285514.1, NP_001272443.1
RefSeq Size	8570 bp
RefSeq ORF	1368 bp
Locus ID	231464
UniProt ID	Q8VEG6
Gene Summary	Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate splice site in the 5' coding region and initiates translation at a downstream start codon, compared to variant 2. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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