Sept6 (NM_001253706) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810035H17Rik; C920001C06Rik; mKIAA0128; Sep6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227639 representing NM_001253706 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGCCGATATAGCTCGCCAGGTGGGCGAAGATTGTCGAACTGTCCCCCTGGCTGGACATGTGG GGTTCGACAGCTTGCCTGACCAGCTGGTGAATAAGTCAGTCAGCCAGGGCTTCTGCTTCAACATCCTGTG CGTGGGGGAGACAGGTTTGGGCAAGTCCACGCTCATGGATACCCTGTTTAACACCAAGTTTGAAGGGGAG CCAGCGACCCACACACAGCCAGGGGTTCAGCTCCAGTCCAATACCTACGACCTCCAGGAGAGCAACGTGG GGTTGAAACTCACAATTGTGAGCACTGTAGGCTTTGGGGATCAGATCAACAAGGAGGACAGCTACAAGCC CATCGTGGAATTCATTGATGCTCAGTTTGAGGCCTACCTACAAGAGGAGCTGAAGATCCGAAGAGTACTG CACTCCTACCATGACTCCCGAATCCATGTCTGCTTGTATTTCATTGCCCCGACAGGACATTCGCTTAAGT CTCTGGACCTAGTGACCATGAAGAAGCTGGATAGTAAGGTGAACATCATTCCCGTTATTGCCAAGTCAGA TGCCATTTCTAAGAGTGAGCTGGCAAAGTTCAAGATCAAAATCACCAGCGAACTTGTGAGCAATGGAGTC CAGATCTATCAGTTCCCCACAGACGATGAGTCGGTGAGCGAGATCAATGGAACCATGAATGCCCACCTGC CGTTTGCTGTTGTTGGCAGCACAGAAGAAGTGAAGATAGGCAACAAGATGATGCGGGCTCGGCAGTATCC TTGGGGGACTGTGCAGGTTGAAAACGAGGCTCACTGCGACTTTGTGAAGCTGCGGGAGATGCTGATTCGG GTCAACATGGAGGATCTGCGGGAGCAGACCCACGCCCGGCACTATGAATTGTACCGCCGCTGTAAGCTGG AGGAGATGGGCTTCAAGGACACCGACCCTGACAGCAAACCATTCAGTTTGCAGGAGACATATGAGGCAAA GAGAAATGAGTTCCTTGGGGAGCTCCAGAAGAAAGAAGAGGAGATGAGGCAGATGTTTGTCCAGAGAGTC AAAGAGAAAGAAGCGGAGCTCAAGGAGGCTGAAAAAGAGCTGCATGAGAAATTTGACCGTCTGAAGAAAC TGCACCAGGAAGAGAAGAAGAAGCTGGAGGATAAGAAGAAATGTCTGGATGAGGAAATGAACGCCTTCAA GCAGAGAAAGGCGGCAGCTGAGCTGCTCCAGTCTCAGGGCTCTCAGGCTGGGGGCTCACAGACTCTGAAA AGGGACAAAGAGAAGAAGAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253706
Insert Size	1284 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253706.1, NP_001240635.1
RefSeq Size	4737 bp
RefSeq ORF	1284 bp
Locus ID	56526
UniProt ID	Q9R1T4
Gene Summary	Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity).[UniProtKB/Swiss-Prot Function]

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