Dus2 (NM_001301176) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310016K04Rik; Dus2l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227398 representing NM_001301176 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGTCCAAAAGAGTATTCCACTAAGGGAGGAATGGGAGCCGCTCTGCTGTCAGACCCTGACAAGA TCGAGAAGATCCTCAGCACTCTTGTTAAAGGGACACACAGACCTGTGACCTGTAAAATCCGAATCCTGCC CTCGCTGGAAGATACCCTGAACTTGGTGAAGCGGATAGAGAGGACTGGCATTTCAGCCATTGCAGTTCAT GGGAGGAATCGTGACGAGCGACCTCAGCACCCGGTCAGCTGTGAAGTTATAAGAGCCATTGCTGAAACCC TCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGACCATATCCAGCAACATGTGGACATAGAAGACTT TCGACAAGCCACGGCTGCCTCCTCTGTGATGGTGGCTCGAGCAGCCATGTGGAACCCCTCCATCTTCCTC AAGGATGGCCTTCGTCCCCTGGAGGAAGTCATGCAGAAGTACATCAGATATGCGGTGCAGTATGATAACC ACTACACCAACACCAAGTACTGCTTGTGCCAAATGCTGCGGGAACAGTTGGAGTCGCCCCAGGGAAGACT GCTTCATGCTGCCCAGTCTTCCCAGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTATGAGGAGACC ATTCGGGAGCTCGATGCTCGGCGAGCTGACCTCCTGGCCAAGACTCCAGAGGCTGTTGAGGAGCCAGCTG AAGATACTTCTGGCATCATTAAAATGGCTATCAGGTTTGACCGGAGAGCATATCCACCCCAGATCACTCC CAAGATGTGCCTGCTGGAATGGTGTCGGAGGGAGAAGCTGCCACAACCTGTGTATGAAACAGTTCAGCGA ACTATAGATCGCATGTTTTGCTCTGTTGTCACTGTTGCGGAACAAAAGTACCAGTCCACCTTGTGGGACA AGTCCAAGAAACTGGCGGAGCAGACTGCAGCAATTGTGTGTCTTAGGAGCCAGGGCCTCCCTGAGGGTCG GCTGGGTGAGGAGAGTCCCTCCTTGAACAAGCGCAAGCGAGAGGCCCCTGACCAAGACCCTGGGGGCCCA AGAGTTCAGGAGCCAGCACTACCTGGGGAGATATGCAAGAAGCCATTTGTAACCTTGGACAGTAGTGAGG AGAATCTTCTGGAAGGCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301176
Insert Size	1143 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301176.1, NP_001288105.1
RefSeq Size	1866 bp
RefSeq ORF	1143 bp
Locus ID	66369
UniProt ID	Q9D7B1
Gene Summary	Dihydrouridine synthase. Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon and uses a downstream translation start codon compared to isoform 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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