Nsfl1c (NM_001291074) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Munc-18c; p47; Stxbp3a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227324 representing NM_001291074 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGAGCGGCAGGACGCGTTGAGGGAGTTCGTGGCGGTGACTGGCACTGAGGAGGACAGGGCCC GTTTCTTCCTGGAGTCGGCTGGCTGGGACCTGCAGATTGCACTTGCAAGCTTTTATGAGGATGGAGGGGA TGAAGACATTGTGACCATTTCACAAGCAACCCCAAGTTCAGTGTCCAGAGGCACAGCTCCTAGTGATAAT AGAGTGACATCCTTCAGAGACCTCATTCATGACCAAGATGAAGAGGAGGAGGAAGAGGAGGGACAGAGGT TTTATGCTGGGGGCTCAGAGAGAAGTGGACAGCAGATTGTTGGTCCTCCCCGGAAGAAAAGTCCCAACGA GCTGGTGGATGATCTGTTTAAGGGTGCCAAAGAGCATGGGGCTGTAGCTGTGGAACGAGTGACCAAGAGC CCTGGAGAGACCAGTAAACCGAGACCATTTGCAGGAGGGGGCTACCGCCTTGGAGCAGCACCAGAGGAAG AGTCTGCCTATGTGGCAGGAGAAAGGAGGCGGCATTCAGGCCAGGATGTTCATGTAGTGCTAAAGCTCTG GAAAACTGGATTCAGCCTAGACAATGGTGACCTTAGAAGCTACCAAGACCCATCCAATGCCCAGTTTCTG GAGTCTATCCGTAGAGGGGAGGTTCCTGCAGAGCTTCGGAGGTTAGCGCATGGTGGGCAGGTGAACCTGG ATATGGAGGATCATCGGGATGAGGACTTCGTGAAGCCCAAGGGAGCTTTCAAAGCCTTCACTGGCGAGGG CCAGAAACTGGGCAGCACGGCCCCCCAGGTATTAAACACCAGCTCTCCAGCCCAACAAGCAGAAAATGAA GCCAAAGCTAGCTCGTCCATCTTAATCAATGAAGCAGAACCTACCACGAACATCCAAATCCGGCTTGCAG ATGGCGGGAGGCTGGTGCAGAAATTCAACCACAGCCACAGGATCAGCGACATCCGGCTCTTCATCGTGGA TGCCCGGCCAGCTATGGCTGCCACCAGTTTTGTCCTTATGACTACCTTCCCTAACAAAGAGCTGGCTGAT GAGAACCAAACCCTGAAGGAAGCCAACCTTCTCAACGCTGTCATCGTGCAGCGGTTAACATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001291074
Insert Size	1113 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291074.1, NP_001278003.1
RefSeq Size	1480 bp
RefSeq ORF	1113 bp
Locus ID	386649
UniProt ID	Q9CZ44
Gene Summary	Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.