Rp2 (NM_001290643) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI662636; Rp2h
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227189 representing NM_001290643 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCTGCTTCACTAAGAGGAGGAAGAGTGAAAAGGCTGAGGGAGAGGAGGAGCAGCCCAAGCTGT ACAGCTGGGACCAGCGGGAGAAGGTTGATCCAAAAGATTACATGTTTAGTGGACTGAAGGATGAAACAGT AGGTCGCTTACCTGGAAAAGTGGCAGGACAACAATTTGTCATTCAAGACTGTGAGAACTGTAACATCTAT ATTTTTGACCACTCAGCTACTATTACCATTGATGACTGCACTAACTGTGTAATTTTTTTGGGACCAGTGA AAGGCAGTGTCTTTTTCCGAAATTGTAGAGATTGCAAGTGCACATTGGCTTGCCAGCAATTTCGTGTGAG AGACTGTAGAAAGTTGGAAGTCTTTTTGTGCTGTGCTACTCAACCCATTATTGAATCTTCCACAAACATC AAGTTTGGCTGTTTTCAATGGTACTACCCTGAATTAGCAGCCCAATTCAAAGATGCAGGCCTCAGTATCT TCAATAACATTTGGAGTCATGTTCATGATTTTACACCTGTGTCAGGAGAGCTCAACTGGAGCCTTCTTCC AGAAAATGCCGTGGTTCAAGACTATGTTCCTATCCCAATGACTGAAGAATTCAAAGCTGTGCGAATTTCC ACAGAAGCCAATAGAAGCATTGTTCCTGTGTCCCGGGGTCAGAGACAGAAGTACAGTGATGAATCATGTC TTGTGGTATTATTTGCCGATGATTATACAACTGCAAATGCCAGGAAACTAATTGATGAGATGGTTGGTAA AGGCTTCTCCCTAGTGCAGACAAAGGAAATGTCAATGAAAACTGAAGACGCCCAAAGGGTTTTCCAGGAA AAGGCATCAGATTTTTTACTTCTTCTAAACAAAGGTCCTGTGATTGCCTTGGAATTTAATGGAGATGACG CTGTACAAGAATGTCACCTTATTGTAAATGGGATGTTCAACGGGACAAAGATGTTTGTATCAGAAAAGAA GGAGACCGCATCTGGAGATGTTGATAGCTTCTATAACTTTGCTGAGATCCAGATGGGGATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290643
Insert Size	1044 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290643.1, NP_001277572.1
RefSeq Size	1399 bp
RefSeq ORF	1044 bp
Locus ID	19889
UniProt ID	Q9EPK2
Gene Summary	Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (a).

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