Mcub (NM_001293644) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9030408N13Rik; Ccdc109b; MCUb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226591 representing NM_001293644 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGAGCTCTGTCCGGCAGGAGGATGCTCCCGAGCGGGCTATGTCTGGGTCGGTGGCAGCTGCTGC GGACGATCCGAGCCCGCGGCCGCGGGGATCCCAGGGAGCTGCCATCCACACCCCAGGTTTTATGTATGAA ACTCTATGGAAATCCAAAATACCACCAAGCACTCCATTATGGGACAGTGGAGCCACAGGATGAAATAACA GTTACTTATAAACACGGCCTTCCCTTGGTAACCCTCACTCTGCCATCCAGAAAAGAGCGCTGCCAGTTTG TAGTCAAGCCGATGTTGTCGACAGTAGGCTCATTCCTGCAGGACTTACAGAATGAGGATAAAGGCATCAA AACTGCAGCCATCATCACGGCAGATGGCAGTGAAATTCCAGCTTCAACATTGATGGACACTTTGCTAATG ACAGATTTTAAGCTCATCATTAACAAACTACGGTATGATATACGGTGTCACAAGAAAGGTCAAAGCTGCA ATAGAAGCCCGGTCGGAAGCCAACACCAGTGGTCTCCTGTGGGCCGGCTTAGCTCTGCTCTCCGTGCAGG GCGGGGCGCTGGCCTGGCTTACTTGGTGGGTGTACTCCTGGGATATCATGGAGCCAGTTACCTTCTTCCT GTCGTTTGCGAATTCCATCGTCTTTTTTGCATACTTCATCATAACCCGGCAGAACTACACATACTCATCC CTCCGGAGCAGGCAGTTTCTTCAGTTCTTCCACAAGAAATCGCAGCGGCGGTGTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293644
Insert Size	759 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293644.1, NP_001280573.1
RefSeq Size	1171 bp
RefSeq ORF	759 bp
Locus ID	66815
UniProt ID	Q810S1
Gene Summary	Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region resulting in a frameshift compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.

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