Med27 (NM_029615) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1500015J03Rik; 2310042P07Rik; AA682045; Crsp8; D2Ertd434e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226309 representing NM_029615 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGTGCTGAGCGTTGGTGTGAACCTGGAGGCCTTTTCGCAGGCTATTAGCGCCATCCAGGCGC TACGCTCTAGCGTGAGTAGGGTCTTCGACTGCCTTAAGGATGGCATGCGCAACAAAGAGACGCTGGAGGG CCGCGAGAAGGCCTTCATCGCGAACTTCCAGGACAACTTGCATTCCGTCAACCGGGACCTCAATGAACTG GAACGTTTGAGCAACCTGGTAGGCAAGCCGTCTGAGAACCACCCTCTTCATAACAGTGGGCTGCTAAGCC TGGATCCTGTGCAGGACAAAACCCCACTCTACAGCCAGCTTCTGCAAGCATACAAATGGTCCAACAAGCT GCAGTACCATGCAGGTCTAGCGTCTGGCCTTTTGAACCAACAGTCCTTGAAGCGCTCTGCAAATCAGATG GGTGTGTCTGCCAAGCGGAGACCGAAGGCCCAGCCCACCACTCTGGTTCTACCACCTCAGTATGTCGATG ACGTGATCAGCCGCATTGACAGAATGTTTCCAGAGATGTCCATTCACTTATCGAGACCCAATGGGACTTC AGCAATGCTTCTGATTCTTATAAACAGCTCTGCTCAGAGCATTACTGGCCCTTGGTTTTGGTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029615
Insert Size	627 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029615.1, NP_083891.1
RefSeq Size	1709 bp
RefSeq ORF	627 bp
Locus ID	68975
UniProt ID	Q9DB40
Gene Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks four 3' exons but instead contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a.

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